Restriction map for pF1KB8993
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AgeI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI          914  GTAC                 GT^AC
AflII         504  CTTAAG               C^TTAAG
AflIII          6  ACATGT               A^C[GA][CT]GT
AhdI          112  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI          319  ATTAAT               AT^TAAT
AsnI          319  ATTAAT               AT^TAAT
AspEI         112  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         838  GGACC                G^G[AT]CC
BamHI         160  GGATCC               G^GATCC
BfrI          504  CTTAAG               C^TTAAG
BglII         454  AGATCT               A^GATCT
BsaI           74  GGTCTC               GGTCTC(1/5)
BseRI         812  CTCCTC               GAGGAG(10/8)
BsiEI        1121  CGATCG               CG[GA][CT]^CG
BspLU11I        6  ACATGT               A^CATGT
BsrI         1176  ACTGG                ACTGG(1/-1)
BstF5I        294  GGATG                GGATG(2/0)
DraIII        937  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1102  CCGTGG               C^C[GA][CT]GG
Eam1105I      112  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          65  CCTCCACTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1043  GATATC               GAT^ATC
FokI          294  GGATG                GGATG(9/13)
HaeIII        934  GGCC                 GG^CC
HincII        885  GTTGAC               GT[CT]^[GA]AC
HindII        885  GTTGAC               GT[CT]^[GA]AC
MspA1I       1136  CAGCTG               C[AC]G^C[GT]G
NdeI          898  CATATG               CA^TATG
NheI         1155  GCTAGC               G^CTAGC
PleI          106  GACTC                GAGTC(4/5)
PshBI         319  ATTAAT               AT^TAAT
PvuI         1121  CGATCG               CGAT^CG
PvuII        1136  CAGCTG               CAG^CTG
RsaI          914  GTAC                 GT^AC
SapI         1159  GCTCTTC              GCTCTTC(1/4)
SfaNI         385  GATGC                GCATC(5/9)
SspI          372  AATATT               AAT^ATT
SwaI          731  ATTTAAAT             ATTT^AAAT
TaqI          405  TCGA                 T^CGA
TthHB8I       405  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          161  GATCC                GGATC(4/5)
              160  GGATC               
BbvI          688  GCTGC                GCAGC(8/12)
             1138  GCTGC               
BsiYI          65  CCTCCACTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               95  CCTTCCCTGGG         
BslI           65  CCTCCACTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               95  CCTTCCCTGGG         
BsmAI          75  GTCTC                GTCTC(1/5)
              196  GTCTC               
BsmFI         104  GGGAC                GGGAC(10/14)
              790  GTCCC               
BstYI         160  GGATCC               [GA]^GATC[CT]
              454  AGATCT              
Cfr13I        838  GGACC                G^G[ACGT]CC
              933  GGGCC               
DraI          732  TTTAAA               TTT^AAA
              876  TTTAAA              
Fnu4HI        688  GCTGC                GC^[ACGT]GC
             1138  GCTGC               
ItaI          688  GCTGC                GC^[ACGT]GC
             1138  GCTGC               
MflI          160  GGATCC               [GA]^GATC[CT]
              454  AGATCT              
Sau96I        838  GGACC                G^G[ACGT]CC
              933  GGGCC               
SmlI           23  CTCAAG               C^T[CT][GA]AG
              504  CTTAAG              
TseI          688  GCTGC                G^C[AT]GC
             1138  GCTGC               
Tsp45I        153  GTCAC                ^GT[GC]AC
              269  GTGAC               
TspRI        1016  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1175  CACTG               
XhoII         160  GGATCC               [GA]^GATC[CT]
              454  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        BsaJI       
BssKI       BstNI       Cac8I       DdeI        DpnII       
EarI        EcoRII      HinfI       HphI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        Sau3AI      ScrFI       TfiI        
Tru9I       Tsp509I     
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