(Circular) (Six-base) MAPSORT of: pF1KB8989.seq Check: 9722 from: 1 to: 4283 pF1KB8989 4283 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AatII G_ACGT'C Cuts at: 985 985 Size: 4283 AccI GT'mk_AC Cuts at: 302 1052 1321 302 Size: 750 269 3264 Fragments arranged by size: 3264 750 269 Acc65I G'GTAC_C Cuts at: 152 1299 152 Size: 1147 3136 AclI AA'CG_TT Cuts at: 4123 4123 Size: 4283 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 997 2108 2540 3287 3555 997 Size: 1111 432 747 268 1725 Fragments arranged by size: 1725 1111 747 432 268 AflIII A'CryG_T Cuts at: 1635 2739 3716 1635 Size: 1104 977 2202 Fragments arranged by size: 2202 1104 977 AgeI A'CCGG_T Cuts at: 2671 2671 Size: 4283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 799 799 Size: 4283 AlwNI CAG_nnn'CTG Cuts at: 1588 3155 1588 Size: 1567 2716 ApaLI G'TGCA_C Cuts at: 1213 3053 1213 Size: 1840 2443 ApoI r'AATT_y Cuts at: 1287 3636 3892 1287 Size: 2349 256 1678 Fragments arranged by size: 2349 1678 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4283 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4283 AvaI C'yCGr_G Cuts at: 148 682 1303 148 Size: 534 621 3128 Fragments arranged by size: 3128 621 534 BamHI G'GATC_C Cuts at: 1308 1308 Size: 4283 BanI G'GyrC_C Cuts at: 152 548 951 1059 1141 1299 1713 1961 1996 Size: 396 403 108 82 158 414 248 35 Cuts at: 1996 152 Size: 2439 Fragments arranged by size: 2439 414 403 396 248 158 108 82 35 BanII G_rGCy'C Cuts at: 195 1297 2327 195 Size: 1102 1030 2151 Fragments arranged by size: 2151 1102 1030 BbeI G_GCGC'C Cuts at: 552 1145 1717 1965 552 Size: 593 572 248 2870 Fragments arranged by size: 2870 593 572 248 BbsI GAAGACnn'nnnn_ Cuts at: 478 841 874 907 985 478 Size: 363 33 33 78 3776 Fragments arranged by size: 3776 363 78 33 33 BciVI GTATCCnnnnn_n' Cuts at: 2181 2942 4283 2181 Size: 761 1341 2181 Fragments arranged by size: 2181 1341 761 BclI T'GATC_A Cuts at: 236 1803 236 Size: 1567 2716 BglII A'GATC_T Cuts at: 1798 3835 1798 Size: 2037 2246 BlpI GC'TnA_GC Cuts at: 271 385 1395 271 Size: 114 1010 3159 Fragments arranged by size: 3159 1010 114 Bme1580I G_kGCm'C Cuts at: 943 956 1217 1908 2001 3057 943 Size: 13 261 691 93 1056 2169 Fragments arranged by size: 2169 1056 691 261 93 13 BmgBI CAC'GTC Cuts at: 1080 1212 1080 Size: 132 4151 BmrI ACTGGGnnnn_n' Cuts at: 1495 1576 1911 4055 1495 Size: 81 335 2144 1723 Fragments arranged by size: 2144 1723 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 198 2650 198 Size: 2452 1831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 352 460 511 541 724 883 916 937 1246 Size: 108 51 30 183 159 33 21 309 Cuts at: 1246 352 Size: 3389 Fragments arranged by size: 3389 309 183 159 108 51 33 30 21 Bpu10I CC'TnA_GC Cuts at: 194 194 Size: 4283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 305 862 1099 1459 2338 2830 3128 305 Size: 557 237 360 879 492 298 1460 Fragments arranged by size: 1460 879 557 492 360 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 3987 3987 Size: 4283 BsaAI yAC'GTr Cuts at: 1023 1636 1023 Size: 613 3670 BsaBI GATnn'nnATC Cuts at: 6 1797 3834 6 Size: 1791 2037 455 Fragments arranged by size: 2037 1791 455 BsaHI Gr'CG_yC Cuts at: 161 549 982 1142 1247 1714 1962 2664 4160 Size: 388 433 160 105 467 248 702 1496 Cuts at: 4160 161 Size: 284 Fragments arranged by size: 1496 702 467 433 388 284 248 160 105 BsaWI w'CCGG_w Cuts at: 1472 1993 2671 2945 3092 3503 1472 Size: 521 678 274 147 411 2252 Fragments arranged by size: 2252 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 196 226 697 727 1021 1051 196 Size: 30 471 30 294 30 3428 Fragments arranged by size: 3428 471 294 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 220 349 673 700 751 763 1252 220 Size: 129 324 27 51 12 489 3251 Fragments arranged by size: 3251 489 324 129 51 27 12 BseYI C'CCAG_C Cuts at: 187 393 663 936 1708 2466 3043 3539 187 Size: 206 270 273 772 758 577 496 931 Fragments arranged by size: 931 772 758 577 496 273 270 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 385 385 Size: 4283 BsiEI CG_ry'CG Cuts at: 104 142 1825 1871 3079 104 Size: 38 1683 46 1208 1308 Fragments arranged by size: 1683 1308 1208 46 38 BsiHKAI G_wGCw'C Cuts at: 1115 1217 1297 2075 2265 3057 1115 Size: 102 80 778 190 792 2341 Fragments arranged by size: 2341 792 778 190 102 80 Bsp1286I G_dGCh'C Cuts at: 195 943 956 1115 1217 1297 1908 2001 2075 Size: 748 13 159 102 80 611 93 74 Cuts at: 2075 2265 2327 3057 195 Size: 190 62 730 1421 Fragments arranged by size: 1421 748 730 611 190 159 102 93 80 74 62 13 BspEI T'CCGG_A Cuts at: 3503 3503 Size: 4283 BspHI T'CATG_A Cuts at: 566 3459 566 Size: 2893 1390 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1196 1333 1547 1849 2230 1196 Size: 137 214 302 381 3249 Fragments arranged by size: 3249 381 302 214 137 BsrBI CCG'CTC Cuts at: 50 138 339 678 963 2575 2629 4113 4278 Size: 88 201 339 285 1612 54 1484 165 Cuts at: 4278 50 Size: 55 Fragments arranged by size: 1612 1484 339 285 201 165 88 55 54 BsrDI GCAATG_nn' Cuts at: 2195 2195 Size: 4283 BsrFI r'CCGG_y Cuts at: 220 594 1180 2281 2462 2671 220 Size: 374 586 1101 181 209 1832 Fragments arranged by size: 1832 1101 586 374 209 181 BssHII G'CGCG_C Cuts at: 1033 1033 Size: 4283 BssSI C'ACGA_G Cuts at: 414 2554 2912 414 Size: 2140 358 1785 Fragments arranged by size: 2140 1785 358 BstAPI GCAn_nnn'nTGC Cuts at: 1221 1660 1221 Size: 439 3844 BstBI TT'CG_AA Cuts at: 2644 2644 Size: 4283 BstXI CCAn_nnnn'nTGG Cuts at: 1128 1128 Size: 4283 BstYI r'GATC_y Cuts at: 1 308 1088 1308 1775 1798 2133 2379 3391 Size: 307 780 220 467 23 335 246 1012 Cuts at: 3391 3477 3489 3835 1 Size: 86 12 346 449 Fragments arranged by size: 1012 780 467 449 346 335 307 246 220 86 23 12 BtgI C'CryG_G Cuts at: 107 1046 107 Size: 939 3344 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2233 2414 2577 2233 Size: 181 163 3939 Fragments arranged by size: 3939 181 163 BtsI GCAGTG_nn' Cuts at: 368 1526 368 Size: 1158 3125 DraI TTT'AAA Cuts at: 561 1282 561 Size: 721 3562 DraIII CAC_nnn'GTG Cuts at: 1026 1026 Size: 4283 DrdI GACnn_nn'nnGTC Cuts at: 1989 2847 1989 Size: 858 3425 EaeI y'GGCC_r Cuts at: 139 596 1868 2042 2433 2460 3805 139 Size: 457 1272 174 391 27 1345 617 Fragments arranged by size: 1345 1272 617 457 391 174 27 EagI C'GGCC_G Cuts at: 139 1868 139 Size: 1729 2554 EarI CTCTTCn'nnn_ Cuts at: 224 2306 2516 224 Size: 2082 210 1991 Fragments arranged by size: 2082 1991 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 361 2491 2801 2947 4093 361 Size: 2130 310 146 1146 551 Fragments arranged by size: 2130 1146 551 310 146 EcoICRI GAG'CTC Cuts at: 1295 1295 Size: 4283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 352 460 511 541 724 883 916 937 997 Size: 108 51 30 183 159 33 21 60 Cuts at: 997 1246 2108 2540 3287 3555 352 Size: 249 862 432 747 268 1080 Fragments arranged by size: 1080 862 747 432 268 249 183 159 108 60 51 33 30 21 EcoO109I rG'GnC_Cy Cuts at: 114 168 1422 114 Size: 54 1254 2975 Fragments arranged by size: 2975 1254 54 EcoRI G'AATT_C Cuts at: 1287 1287 Size: 4283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 828 2302 828 Size: 1474 2809 FspI TGC'GCA Cuts at: 451 1000 2064 451 Size: 549 1064 2670 Fragments arranged by size: 2670 1064 549 HaeII r_GCGC'y Cuts at: 498 552 640 703 1145 1717 1965 2987 3966 Size: 54 88 63 442 572 248 1022 979 Cuts at: 3966 498 Size: 815 Fragments arranged by size: 1022 979 815 572 442 248 88 63 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 196 228 697 729 2125 2155 2157 2187 196 Size: 32 469 32 1396 30 2 30 2292 Fragments arranged by size: 2292 1396 469 32 32 30 30 2 HincII GTy'rAC Cuts at: 465 528 1322 465 Size: 63 794 3426 Fragments arranged by size: 3426 794 63 KasI G'GCGC_C Cuts at: 548 1141 1713 1961 548 Size: 593 572 248 2870 Fragments arranged by size: 2870 593 572 248 KpnI G_GTAC'C Cuts at: 156 1303 156 Size: 1147 3136 MluI A'CGCG_T Cuts at: 3716 3716 Size: 4283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 224 611 695 1223 2954 3138 224 Size: 387 84 528 1731 184 1369 Fragments arranged by size: 1731 1369 528 387 184 84 MscI TGG'CCA Cuts at: 2044 2044 Size: 4283 MslI CAynn'nnrTG Cuts at: 1126 2272 2399 1126 Size: 1146 127 3010 Fragments arranged by size: 3010 1146 127 MspA1I CmG'CkG Cuts at: 187 202 315 330 697 779 1048 1394 1708 Size: 15 113 15 367 82 269 346 314 Cuts at: 1708 2068 3081 3326 187 Size: 360 1013 245 1144 Fragments arranged by size: 1144 1013 367 360 346 314 269 245 113 82 15 15 NaeI GCC'GGC Cuts at: 222 596 1182 2464 222 Size: 374 586 1282 2041 Fragments arranged by size: 2041 1282 586 374 NarI GG'CG_CC Cuts at: 549 1142 1714 1962 549 Size: 593 572 248 2870 Fragments arranged by size: 2870 593 572 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4283 NgoMIV G'CCGG_C Cuts at: 220 594 1180 2462 220 Size: 374 586 1282 2041 Fragments arranged by size: 2041 1282 586 374 NotI GC'GGCC_GC Cuts at: 139 139 Size: 4283 NspI r_CATG'y Cuts at: 1336 1836 2743 1336 Size: 500 907 2876 Fragments arranged by size: 2876 907 500 PciI A'CATG_T Cuts at: 2739 2739 Size: 4283 PfoI T'CCnGG_A Cuts at: 278 278 Size: 4283 PmeI GTTT'AAAC Cuts at: 1282 1282 Size: 4283 PmlI CAC'GTG Cuts at: 1023 1023 Size: 4283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 3422 Size: 32 4251 PpuMI rG'GwC_Cy Cuts at: 114 114 Size: 4283 PshAI GACnn'nnGTC Cuts at: 1048 1048 Size: 4283 PstI C_TGCA'G Cuts at: 1330 2015 1330 Size: 685 3598 PvuI CG_AT'CG Cuts at: 104 104 Size: 4283 PvuII CAG'CTG Cuts at: 315 330 1708 2068 315 Size: 15 1378 360 2530 Fragments arranged by size: 2530 1378 360 15 RsrII CG'GwC_CG Cuts at: 2478 2478 Size: 4283 SacI G_AGCT'C Cuts at: 1297 1297 Size: 4283 SacII CC_GC'GG Cuts at: 1049 1049 Size: 4283 SalI G'TCGA_C Cuts at: 1320 1320 Size: 4283 SapI GCTCTTCn'nnn_ Cuts at: 224 2306 2516 224 Size: 2082 210 1991 Fragments arranged by size: 2082 1991 210 SbfI CC_TGCA'GG Cuts at: 1330 1330 Size: 4283 ScaI AGT'ACT Cuts at: 3800 3800 Size: 4283 SexAI A'CCwGG_T Cuts at: 248 248 Size: 4283 SfcI C'TryA_G Cuts at: 33 1326 2011 3004 3195 33 Size: 1293 685 993 191 1121 Fragments arranged by size: 1293 1121 993 685 191 SfoI GGC'GCC Cuts at: 550 1143 1715 1963 550 Size: 593 572 248 2870 Fragments arranged by size: 2870 593 572 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4283 SmaI CCC'GGG Cuts at: 150 1305 150 Size: 1155 3128 SmlI C'TyrA_G Cuts at: 320 877 1114 1438 2353 2845 3107 320 Size: 557 237 324 915 492 262 1496 Fragments arranged by size: 1496 915 557 492 324 262 237 SpeI A'CTAG_T Cuts at: 67 67 Size: 4283 SphI G_CATG'C Cuts at: 1336 1836 1336 Size: 500 3783 StuI AGG'CCT Cuts at: 811 811 Size: 4283 StyI C'CwwG_G Cuts at: 107 818 839 884 1417 1771 107 Size: 711 21 45 533 354 2619 Fragments arranged by size: 2619 711 533 354 45 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1866 1999 2458 2667 1866 Size: 133 459 209 3482 Fragments arranged by size: 3482 459 209 133 TatI w'GTAC_w Cuts at: 3798 3798 Size: 4283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3800 3832 3800 Size: 32 4251 Tth111I GACn'n_nGTC Cuts at: 2080 2080 Size: 4283 XbaI T'CTAG_A Cuts at: 1314 1314 Size: 4283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 825 891 1128 1778 825 Size: 66 237 650 3330 Fragments arranged by size: 3330 650 237 66 XmaI C'CCGG_G Cuts at: 148 1303 148 Size: 1155 3128 XmnI GAAnn'nnTTC Cuts at: 811 811 Size: 4283 ZraI GAC'GTC Cuts at: 983 983 Size: 4283 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI