Restriction map for pF1KB8984
 (Linear) (Six-base) MAPSORT of: KIBB8984.seq  Check: 8004  from: 1  to: 1158



KIBB8984.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1056    1158 
   Size:       1056     102
 
AfeI AGC'GCT

Cuts at:      0     349    1158 
   Size:        349     809
 
AleI CACnn'nnGTG

Cuts at:      0      28    1158 
   Size:         28    1130
 
AlwNI CAG_nnn'CTG

Cuts at:      0     629    1158 
   Size:        629     529
 
ApaI G_GGCC'C

Cuts at:      0     398     531    1005    1158 
   Size:        398     133     474     153

  Fragments arranged by size: 

                 474     398     153     133
 
AscI GG'CGCG_CC

Cuts at:      0     130    1158 
   Size:        130    1028
 
AvaI C'yCGr_G

Cuts at:      0     254     934    1158 
   Size:        254     680     224

  Fragments arranged by size: 

                 680     254     224
 
BanI G'GyrC_C

Cuts at:      0     187     541    1158 
   Size:        187     354     617

  Fragments arranged by size: 

                 617     354     187
 
BanII G_rGCy'C

Cuts at:      0      40     205     398     531     667    1005    1076    1158 
   Size:         40     165     193     133     136     338      71      82

  Fragments arranged by size: 

                 338     193     165     136     133      82      71      40
            
 
BbeI G_GCGC'C

Cuts at:      0     545    1158 
   Size:        545     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     864     888    1158 
   Size:        864      24     270

  Fragments arranged by size: 

                 864     270      24
 
BbvCI CC'TCA_GC

Cuts at:      0     622    1158 
   Size:        622     536
 
BglI GCCn_nnn'nGGC

Cuts at:      0     412     662    1158 
   Size:        412     250     496

  Fragments arranged by size: 

                 496     412     250
 
BlpI GC'TnA_GC

Cuts at:      0     361    1158 
   Size:        361     797
 
Bme1580I G_kGCm'C

Cuts at:      0     190     398     531    1005    1158 
   Size:        190     208     133     474     153

  Fragments arranged by size: 

                 474     208     190     153     133
 
Bpu10I CC'TnA_GC

Cuts at:      0     112     199     622    1158 
   Size:        112      87     423     536

  Fragments arranged by size: 

                 536     423     112      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     892    1158 
   Size:        892     266
 
BsaAI yAC'GTr

Cuts at:      0      30    1158 
   Size:         30    1128
 
BsaHI Gr'CG_yC

Cuts at:      0     313     542    1158 
   Size:        313     229     616

  Fragments arranged by size: 

                 616     313     229
 
BsaWI w'CCGG_w

Cuts at:      0     160     688    1158 
   Size:        160     528     470

  Fragments arranged by size: 

                 528     470     160
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1002    1158 
   Size:       1002     156
 
BseYI C'CCAG_C

Cuts at:      0     183     243     465     524     530     971    1158 
   Size:        183      60     222      59       6     441     187

  Fragments arranged by size: 

                 441     222     187     183      60      59       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33    1158 
   Size:         33    1125
 
BsiEI CG_ry'CG

Cuts at:      0     127     209     682    1158 
   Size:        127      82     473     476

  Fragments arranged by size: 

                 476     473     127      82
 
BsiHKAI G_wGCw'C

Cuts at:      0     754    1158 
   Size:        754     404
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     190     205     398     531     667     754    1005 
   Size:         40     150      15     193     133     136      87     251

Cuts at:   1005    1076    1158 
   Size:         71      82

  Fragments arranged by size: 

                 251     193     150     136     133      87      82      71
                  40      15
 
BspHI T'CATG_A

Cuts at:      0     728    1158 
   Size:        728     430
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     188    1056    1158 
   Size:        188     868     102

  Fragments arranged by size: 

                 868     188     102
 
BsrFI r'CCGG_y

Cuts at:      0      48      76     126     565    1158 
   Size:         48      28      50     439     593

  Fragments arranged by size: 

                 593     439      50      48      28
 
BssHII G'CGCG_C

Cuts at:      0     130     226     452    1158 
   Size:        130      96     226     706

  Fragments arranged by size: 

                 706     226     130      96
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     506    1158 
   Size:        506     652
 
BstEII G'GTnAC_C

Cuts at:      0     156     615    1158 
   Size:        156     459     543

  Fragments arranged by size: 

                 543     459     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     342    1158 
   Size:        342     816
 
BtgI C'CryG_G

Cuts at:      0     335     341     676     945    1109    1158 
   Size:        335       6     335     269     164      49

  Fragments arranged by size: 

                 335     335     269     164      49       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     290    1158 
   Size:        290     868
 
BtsI GCAGTG_nn'

Cuts at:      0     813    1158 
   Size:        813     345
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     868    1158 
   Size:        868     290
 
EaeI y'GGCC_r

Cuts at:      0      50      74     170     206     338     679     743    1158 
   Size:         50      24      96      36     132     341      64     415

  Fragments arranged by size: 

                 415     341     132      96      64      50      36      24
            
 
EagI C'GGCC_G

Cuts at:      0     206     679    1158 
   Size:        206     473     479

  Fragments arranged by size: 

                 479     473     206
 
EarI CTCTTCn'nnn_

Cuts at:      0     451    1158 
   Size:        451     707
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1158 
   Size:         19    1139
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     984    1158 
   Size:        984     174
 
EcoO109I rG'GnC_Cy

Cuts at:      0     395     819     993    1001    1002    1158 
   Size:        395     424     174       8       1     156

  Fragments arranged by size: 

                 424     395     174     156       8       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     912    1158 
   Size:        912     246
 
FseI GG_CCGG'CC

Cuts at:      0      52    1158 
   Size:         52    1106
 
HaeII r_GCGC'y

Cuts at:      0     351     361     418     545     657    1109    1158 
   Size:        351      10      57     127     112     452      49

  Fragments arranged by size: 

                 452     351     127     112      57      49      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     896     928    1158 
   Size:        896      32     230

  Fragments arranged by size: 

                 896     230      32
 
KasI G'GCGC_C

Cuts at:      0     541    1158 
   Size:        541     617
 
MslI CAynn'nnrTG

Cuts at:      0      28      64     340     733     745    1158 
   Size:         28      36     276     393      12     413

  Fragments arranged by size: 

                 413     393     276      36      28      12
 
MspA1I CmG'CkG

Cuts at:      0     387     401     503     773    1100    1158 
   Size:        387      14     102     270     327      58

  Fragments arranged by size: 

                 387     327     270     102      58      14
 
NaeI GCC'GGC

Cuts at:      0      50     567    1158 
   Size:         50     517     591

  Fragments arranged by size: 

                 591     517      50
 
NarI GG'CG_CC

Cuts at:      0     542    1158 
   Size:        542     616
 
NcoI C'CATG_G

Cuts at:      0     335    1158 
   Size:        335     823
 
NgoMIV G'CCGG_C

Cuts at:      0      48     565    1158 
   Size:         48     517     593

  Fragments arranged by size: 

                 593     517      48
 
NspI r_CATG'y

Cuts at:      0     522     639    1158 
   Size:        522     117     519

  Fragments arranged by size: 

                 522     519     117
 
PasI CC'CwG_GG

Cuts at:      0     102     866    1158 
   Size:        102     764     292

  Fragments arranged by size: 

                 764     292     102
 
PfoI T'CCnGG_A

Cuts at:      0     857     982    1158 
   Size:        857     125     176

  Fragments arranged by size: 

                 857     176     125
 
PpuMI rG'GwC_Cy

Cuts at:      0     819     993    1158 
   Size:        819     174     165

  Fragments arranged by size: 

                 819     174     165
 
PshAI GACnn'nnGTC

Cuts at:      0     736    1158 
   Size:        736     422
 
PspOMI G'GGCC_C

Cuts at:      0     394     527    1001    1158 
   Size:        394     133     474     157

  Fragments arranged by size: 

                 474     394     157     133
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      10      42    1158 
   Size:         10      32    1116

  Fragments arranged by size: 

                1116      32      10
 
PvuII CAG'CTG

Cuts at:      0     387     503    1158 
   Size:        387     116     655

  Fragments arranged by size: 

                 655     387     116
 
SanDI GG'GwC_CC

Cuts at:      0     993    1158 
   Size:        993     165
 
ScaI AGT'ACT

Cuts at:      0     490    1158 
   Size:        490     668
 
SfoI GGC'GCC

Cuts at:      0     543    1158 
   Size:        543     615
 
SmlI C'TyrA_G

Cuts at:      0     907    1158 
   Size:        907     251
 
SphI G_CATG'C

Cuts at:      0     522    1158 
   Size:        522     636
 
StyI C'CwwG_G

Cuts at:      0     335     997    1158 
   Size:        335     662     161

  Fragments arranged by size: 

                 662     335     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     603     796    1158 
   Size:        603     193     362

  Fragments arranged by size: 

                 603     362     193
 
TatI w'GTAC_w

Cuts at:      0     488    1158 
   Size:        488     670
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     180    1158 
   Size:        180     978
 

 Enzymes that do cut:

     AarI     AfeI     AleI    AlwNI     ApaI     AscI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI   BssHII   BstAPI   BstEII
    BstXI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI    EcoNI EcoO109I     FalI     FseI    HaeII    Hin4I     KasI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PfoI    PpuMI    PshAI   PspOMI     PsrI    PvuII    SanDI     ScaI
     SfoI     SmlI     SphI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaBI    BsaXI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI    BssSI    BstBI
    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII  EcoICRI
  Eco57MI    EcoRI    EcoRV     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
     PsiI    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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