Restriction map for pF1KB8970
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        BamHI       BbrPI       
BcgI        BciVI       BclI        BfrI        BlpI        
Bpu1102I    BsaAI       BsaI        BseAI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeII       MluI        
MroI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfuI        
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AluI          779  AGCT                 AG^CT
AlwI          783  GGATC                GGATC(4/5)
AvaI          129  CTCGGG               C^[CT]CG[GA]G
AviII         665  TGCGCA               TGC^GCA
AvrII         399  CCTAGG               C^CTAGG
BalI          371  TGGCCA               TGG^CCA
BbeI          658  GGCGCC               GGCGC^C
BbvCI         763  CCTCAGC              CCTCAGC(-5/-2)
BfaI          400  CTAG                 C^TAG
BglI           89  GCCTCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         979  AGATCT               A^GATCT
BlnI          399  CCTAGG               C^CTAGG
BsaWI         269  ACCGGA               [AT]^CCGG[AT]
BsoBI         129  CTCGGG               C^[CT]CG[GA]G
BspMI         101  ACCTGC               ACCTGC(4/8)
BssSI         565  CACGAG               CACGAG(-5/-1)
BstAPI        539  GCACCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        207  GGTCACC              G^GT[ACGT]ACC
BstF5I        873  GGATG                GGATG(2/0)
BstPI         207  GGTCACC              G^GT[ACGT]ACC
BstYI         979  AGATCT               [GA]^GATC[CT]
DraII         892  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          374  CCATGG               C^C[GA][CT]GG
EaeI          371  TGGCCA               [CT]^GGCC[GA]
EcoNI         713  CCTGCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      892  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       207  GGTCACC              G^GT[ACGT]ACC
FokI          873  GGATG                GGATG(9/13)
FspI          665  TGCGCA               TGC^GCA
KasI          658  GGCGCC               G^GCGCC
MaeI          400  CTAG                 C^TAG
MaeIII        208  GTCAC                ^GT[ACGT]AC
MfeI          947  CAATTG               C^AATTG
MflI          979  AGATCT               [GA]^GATC[CT]
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
MseI          869  TTAA                 T^TAA
MunI          947  CAATTG               C^AATTG
NarI          658  GGCGCC               GG^CGCC
NcoI          374  CCATGG               C^CATGG
NspI          185  ACATGC               [GA]CATG^[CT]
PleI          273  GAGTC                GAGTC(4/5)
PpuMI         892  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          694  CTGCAG               CTGCA^G
ScaI          527  AGTACT               AGT^ACT
SexAI         541  ACCTGGT              A^CC[AT]GGT
SfaNI         681  GATGC                GCATC(5/9)
SfcI          694  CTGCAG               C^T[GA][CT]AG
SfoI          658  GGCGCC               GGC^GCC
SgrAI         685  CACCGGCG             C[GA]^CCGG[CT]G
TaqI          355  TCGA                 T^CGA
TfiI         1066  GAATC                G^A[AT]TC
Tru9I         869  TTAA                 T^TAA
Tsp45I        208  GTCAC                ^GT[GC]AC
TthHB8I       355  TCGA                 T^CGA
XhoII         979  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          152  GGCGTC               G[GA]^CG[CT]C
              658  GGCGCC              
AlwNI         720  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              766  CAGCCCCTG           
BanII         346  GGGCTC               G[GA]GC[CT]^C
              573  GGGCTC              
BbsI          910  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
BpuAI         910  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
BsaBI         929  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              963  GATTTTTATC          
BsaHI         152  GGCGTC               G[GA]^CG[CT]C
              658  GGCGCC              
BsmFI         722  GGGAC                GGGAC(10/14)
              759  GTCCC               
DpnII         784  GATC                 ^GATC
              980  GATC                
DraIII        540  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              822  CACTATGTG           
EarI           76  CTCTTC               CTCTTC(1/4)
              817  CTCTTC              
HgaI          153  GCGTC                GACGC(5/10)
              757  GCGTC               
Hin1I         152  GGCGTC               G[GA]^CG[CT]C
              658  GGCGCC              
HinfI         273  GAGTC                G^A[ACGT]TC
             1066  GAATC               
Ksp632I        76  CTCTTC               CTCTTC(1/4)
              817  CTCTTC              
MamI          929  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              963  GATTTTTATC          
MboI          784  GATC                 ^GATC
              980  GATC                
NdeII         784  GATC                 ^GATC
              980  GATC                
SapI           75  GCTCTTC              GCTCTTC(1/4)
              816  GCTCTTC             
Sau3AI        784  GATC                 ^GATC
              980  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AfaI        AspHI       AvaII       
BanI        BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrFI       BsrI        BssHII      
BssKI       BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        EcoRII      
EcoT14I     FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       RsaI        Sau96I      ScrFI       
StyI        TseI        Tsp509I     TspRI       
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