Restriction map for pF1KB8970
 (Circular) (Six-base) MAPSORT of: pF1KB8970.seq  Check: 3280  from: 1  to: 4190



pF1KB8970  4190 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:   1228    1228 
   Size:       4190
 
Acc65I G'GTAC_C

Cuts at:   1206    1206 
   Size:       4190
 
AclI AA'CG_TT

Cuts at:   4030    4030 
   Size:       4190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    171    1028    2015    2447    3194    3462     171 
   Size:        857     987     432     747     268     899

  Fragments arranged by size: 

                 987     899     857     747     432     268
 
AflIII A'CryG_T

Cuts at:   1542    2646    3623    1542 
   Size:       1104     977    2109

  Fragments arranged by size: 

                2109    1104     977
 
AgeI A'CCGG_T

Cuts at:   2578    2578 
   Size:       4190
 
AleI CACnn'nnGTG

Cuts at:    145     492     145 
   Size:        347    3843
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    510     510 
   Size:       4190
 
AlwNI CAG_nnn'CTG

Cuts at:    833     879    1495    3062     833 
   Size:         46     616    1567    1961

  Fragments arranged by size: 

                1961    1567     616      46
 
ApaLI G'TGCA_C

Cuts at:   2960    2960 
   Size:       4190
 
ApoI r'AATT_y

Cuts at:   1194    3543    3799    1194 
   Size:       2349     256    1585

  Fragments arranged by size: 

                2349    1585     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4190
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4190
 
AvaI C'yCGr_G

Cuts at:    237    1210     237 
   Size:        973    3217
 
AvrII C'CTAG_G

Cuts at:    507     507 
   Size:       4190
 
BamHI G'GATC_C

Cuts at:   1215    1215 
   Size:       4190
 
BanI G'GyrC_C

Cuts at:    512     646     766     809    1206    1620    1868    1903     512 
   Size:        134     120      43     397     414     248      35    2799

  Fragments arranged by size: 

                2799     414     397     248     134     120      43      35
            
 
BanII G_rGCy'C

Cuts at:    458     685    1204    2234     458 
   Size:        227     519    1030    2414

  Fragments arranged by size: 

                2414    1030     519     227
 
BbeI G_GCGC'C

Cuts at:    770    1624    1872     770 
   Size:        854     248    3088

  Fragments arranged by size: 

                3088     854     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    279    1025     279 
   Size:        746    3444
 
BbvCI CC'TCA_GC

Cuts at:    872     872 
   Size:       4190
 
BciVI GTATCCnnnnn_n'

Cuts at:   2088    2849    4190    2088 
   Size:        761    1341    2088

  Fragments arranged by size: 

                2088    1341     761
 
BclI T'GATC_A

Cuts at:   1710    1710 
   Size:       4190
 
BglI GCCn_nnn'nGGC

Cuts at:    203     203 
   Size:       4190
 
BglII A'GATC_T

Cuts at:   1087    1705    3742    1087 
   Size:        618    2037    1535

  Fragments arranged by size: 

                2037    1535     618
 
BlpI GC'TnA_GC

Cuts at:   1302    1302 
   Size:       4190
 
Bme1580I G_kGCm'C

Cuts at:    515    1815    1908    2964     515 
   Size:       1300      93    1056    1741

  Fragments arranged by size: 

                1741    1300    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1402    1483    1818    3962    1402 
   Size:         81     335    2144    1630

  Fragments arranged by size: 

                2144    1630     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    699    2557     699 
   Size:       1858    2332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    511     702     901    1076     511 
   Size:        191     199     175    3625

  Fragments arranged by size: 

                3625     199     191     175
 
Bpu10I CC'TnA_GC

Cuts at:    343     872     343 
   Size:        529    3661
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1366    2245    2737    3035    1366 
   Size:        879     492     298    2521

  Fragments arranged by size: 

                2521     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3894    3894 
   Size:       4190
 
BsaAI yAC'GTr

Cuts at:   1543    1543 
   Size:       4190
 
BsaBI GATnn'nnATC

Cuts at:      6    1041    1075    1704    3741       6 
   Size:       1035      34     629    2037     455

  Fragments arranged by size: 

                2037    1035     629     455      34
 
BsaHI Gr'CG_yC

Cuts at:    261     767    1621    1869    2571    4067     261 
   Size:        506     854     248     702    1496     384

  Fragments arranged by size: 

                1496     854     702     506     384     248
 
BsaWI w'CCGG_w

Cuts at:    377    1379    1900    2578    2852    2999    3410     377 
   Size:       1002     521     678     274     147     411    1157

  Fragments arranged by size: 

                1157    1002     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    458     488     802     832     458 
   Size:         30     314      30    3816

  Fragments arranged by size: 

                3816     314      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    450     691     898     450 
   Size:        241     207    3742

  Fragments arranged by size: 

                3742     241     207
 
BseYI C'CCAG_C

Cuts at:    243     327     696    1615    2373    2950    3446     243 
   Size:         84     369     919     758     577     496     987

  Fragments arranged by size: 

                 987     919     758     577     496     369      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    652     807    1173     652 
   Size:        155     366    3669

  Fragments arranged by size: 

                3669     366     155
 
BsiEI CG_ry'CG

Cuts at:    104    1732    1778    2986     104 
   Size:       1628      46    1208    1308

  Fragments arranged by size: 

                1628    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    185     256     713     926    1204    1982    2172    2964     185 
   Size:         71     457     213     278     778     190     792    1411

  Fragments arranged by size: 

                1411     792     778     457     278     213     190      71
            
 
Bsp1286I G_dGCh'C

Cuts at:    185     256     458     515     685     713     926    1204    1815 
   Size:         71     202      57     170      28     213     278     611

Cuts at:   1815    1908    1982    2172    2234    2964     185 
   Size:         93      74     190      62     730    1411

  Fragments arranged by size: 

                1411     730     611     278     213     202     190     170
                  93      74      71      62      57      28
 
BspEI T'CCGG_A

Cuts at:   3410    3410 
   Size:       4190
 
BspHI T'CATG_A

Cuts at:   3366    3366 
   Size:       4190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    218    1240    1454    1756    2137     218 
   Size:       1022     214     302     381    2271

  Fragments arranged by size: 

                2271    1022     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2482    2536    4020    4185      50 
   Size:       2432      54    1484     165      55

  Fragments arranged by size: 

                2432    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2102    2102 
   Size:       4190
 
BsrFI r'CCGG_y

Cuts at:    193     794    1148    2188    2369    2578     193 
   Size:        601     354    1040     181     209    1805

  Fragments arranged by size: 

                1805    1040     601     354     209     181
 
BssHII G'CGCG_C

Cuts at:    232     599     846     232 
   Size:        367     247    3576

  Fragments arranged by size: 

                3576     367     247
 
BssSI C'ACGA_G

Cuts at:    673    2461    2819     673 
   Size:       1788     358    2044

  Fragments arranged by size: 

                2044    1788     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    653    1567     653 
   Size:        914    3276
 
BstBI TT'CG_AA

Cuts at:   2551    2551 
   Size:       4190
 
BstEII G'GTnAC_C

Cuts at:    315     315 
   Size:       4190
 
BstXI CCAn_nnnn'nTGG

Cuts at:    147     489     726    1170     147 
   Size:        342     237     444    3167

  Fragments arranged by size: 

                3167     444     342     237
 
BstYI r'GATC_y

Cuts at:      1    1087    1215    1682    1705    2040    2286    3298    3384 
   Size:       1086     128     467      23     335     246    1012      86

Cuts at:   3384    3396    3742       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1086    1012     467     449     346     335     246     128
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    482     482 
   Size:       4190
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    802    2140    2321    2484     802 
   Size:       1338     181     163    2508

  Fragments arranged by size: 

                2508    1338     181     163
 
BtsI GCAGTG_nn'

Cuts at:    265     466    1433     265 
   Size:        201     967    3022

  Fragments arranged by size: 

                3022     967     201
 
DraI TTT'AAA

Cuts at:   1189    1189 
   Size:       4190
 
DraIII CAC_nnn'GTG

Cuts at:    653     935     653 
   Size:        282    3908
 
DrdI GACnn_nn'nnGTC

Cuts at:   1896    2754    1896 
   Size:        858    3332
 
EaeI y'GGCC_r

Cuts at:    479    1775    1949    2340    2367    3712     479 
   Size:       1296     174     391      27    1345     957

  Fragments arranged by size: 

                1345    1296     957     391     174      27
 
EagI C'GGCC_G

Cuts at:   1775    1775 
   Size:       4190
 
EarI CTCTTCn'nnn_

Cuts at:    190     931    2213    2423     190 
   Size:        741    1282     210    1957

  Fragments arranged by size: 

                1957    1282     741     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2398    2708    2854    4000    2398 
   Size:        310     146    1146    2588

  Fragments arranged by size: 

                2588    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1202    1202 
   Size:       4190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    171     511     702     901    1028    1076    2015    2447    3194 
   Size:        340     191     199     127      48     939     432     747

Cuts at:   3194    3462     171 
   Size:        268     899

  Fragments arranged by size: 

                 939     899     747     432     340     268     199     191
                 127      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:    825     825 
   Size:       4190
 
EcoO109I rG'GnC_Cy

Cuts at:   1001    1329    1001 
   Size:        328    3862
 
EcoRI G'AATT_C

Cuts at:   1194    1194 
   Size:       4190
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2209    2209 
   Size:       4190
 
FspI TGC'GCA

Cuts at:    775    1971     775 
   Size:       1196    2994
 
HaeII r_GCGC'y

Cuts at:    581     733     770     913    1624    1872    2894    3873     581 
   Size:        152      37     143     711     248    1022     979     898

  Fragments arranged by size: 

                1022     979     898     711     248     152     143      37
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    521     553    2032    2062    2064    2094     521 
   Size:         32    1479      30       2      30    2617

  Fragments arranged by size: 

                2617    1479      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1229    1229 
   Size:       4190
 
KasI G'GCGC_C

Cuts at:    766    1620    1868     766 
   Size:        854     248    3088

  Fragments arranged by size: 

                3088     854     248
 
KpnI G_GTAC'C

Cuts at:   1210    1210 
   Size:       4190
 
MfeI C'AATT_G

Cuts at:   1055    1055 
   Size:       4190
 
MluI A'CGCG_T

Cuts at:   3623    3623 
   Size:       4190
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2861    3045    2861 
   Size:        184    4006
 
MscI TGG'CCA

Cuts at:    481    1951     481 
   Size:       1470    2720
 
MslI CAynn'nnrTG

Cuts at:    145     298     492    1168    2179    2306     145 
   Size:        153     194     676    1011     127    2029

  Fragments arranged by size: 

                2029    1011     676     194     153     127
 
MspA1I CmG'CkG

Cuts at:    128     276     807    1301    1615    1975    2988    3233     128 
   Size:        148     531     494     314     360    1013     245    1085

  Fragments arranged by size: 

                1085    1013     531     494     360     314     245     148
            
 
NaeI GCC'GGC

Cuts at:   2371    2371 
   Size:       4190
 
NarI GG'CG_CC

Cuts at:    767    1621    1869     767 
   Size:        854     248    3088

  Fragments arranged by size: 

                3088     854     248
 
NcoI C'CATG_G

Cuts at:    482     482 
   Size:       4190
 
NgoMIV G'CCGG_C

Cuts at:   2369    2369 
   Size:       4190
 
NspI r_CATG'y

Cuts at:    297    1243    1743    2650     297 
   Size:        946     500     907    1837

  Fragments arranged by size: 

                1837     946     907     500
 
PasI CC'CwG_GG

Cuts at:    219     219 
   Size:       4190
 
PciI A'CATG_T

Cuts at:   2646    2646 
   Size:       4190
 
PmeI GTTT'AAAC

Cuts at:   1189    1189 
   Size:       4190
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    127     159    3329    3361     127 
   Size:         32    3170      32     956

  Fragments arranged by size: 

                3170     956      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1001    1001 
   Size:       4190
 
PstI C_TGCA'G

Cuts at:    806    1237    1922     806 
   Size:        431     685    3074

  Fragments arranged by size: 

                3074     685     431
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4190
 
PvuII CAG'CTG

Cuts at:   1615    1975    1615 
   Size:        360    3830
 
RsrII CG'GwC_CG

Cuts at:   2385    2385 
   Size:       4190
 
SacI G_AGCT'C

Cuts at:   1204    1204 
   Size:       4190
 
SalI G'TCGA_C

Cuts at:   1227    1227 
   Size:       4190
 
SapI GCTCTTCn'nnn_

Cuts at:    190     931    2213    2423     190 
   Size:        741    1282     210    1957

  Fragments arranged by size: 

                1957    1282     741     210
 
SbfI CC_TGCA'GG

Cuts at:   1237    1237 
   Size:       4190
 
ScaI AGT'ACT

Cuts at:    637    3707     637 
   Size:       3070    1120
 
SexAI A'CCwGG_T

Cuts at:    649     649 
   Size:       4190
 
SfcI C'TryA_G

Cuts at:     33     802    1233    1918    2911    3102      33 
   Size:        769     431     685     993     191    1121

  Fragments arranged by size: 

                1121     993     769     685     431     191
 
SfoI GGC'GCC

Cuts at:    768    1622    1870     768 
   Size:        854     248    3088

  Fragments arranged by size: 

                3088     854     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4190
 
SgrAI Cr'CCGG_yG

Cuts at:    794     794 
   Size:       4190
 
SmaI CCC'GGG

Cuts at:   1212    1212 
   Size:       4190
 
SmlI C'TyrA_G

Cuts at:   1345    2260    2752    3014    1345 
   Size:        915     492     262    2521

  Fragments arranged by size: 

                2521     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4190
 
SphI G_CATG'C

Cuts at:   1243    1743    1243 
   Size:        500    3690
 
StyI C'CwwG_G

Cuts at:    482     507    1066    1324    1678     482 
   Size:         25     559     258     354    2994

  Fragments arranged by size: 

                2994     559     354     258      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    273    1773    1906    2365    2574     273 
   Size:       1500     133     459     209    1889

  Fragments arranged by size: 

                1889    1500     459     209     133
 
TatI w'GTAC_w

Cuts at:    635    3705     635 
   Size:       3070    1120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    458     490     802     834    3707    3739     458 
   Size:         32     312      32    2873      32     909

  Fragments arranged by size: 

                2873     909     312      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1987    1987 
   Size:       4190
 
XbaI T'CTAG_A

Cuts at:   1221    1221 
   Size:       4190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1685    1685 
   Size:       4190
 
XmaI C'CCGG_G

Cuts at:   1210    1210 
   Size:       4190
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI    PpuMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI
     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI
    BsrGI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI     SfiI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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