Restriction map for pF1KB8970
 (Linear) (Six-base) MAPSORT of: KIBB8970.seq  Check: 7185  from: 1  to: 1077



KIBB8970.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     920    1077 
   Size:         63     857     157

  Fragments arranged by size: 

                 857     157      63
 
AleI CACnn'nnGTG

Cuts at:      0      37     384    1077 
   Size:         37     347     693

  Fragments arranged by size: 

                 693     347      37
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     402    1077 
   Size:        402     675
 
AlwNI CAG_nnn'CTG

Cuts at:      0     725     771    1077 
   Size:        725      46     306

  Fragments arranged by size: 

                 725     306      46
 
AvaI C'yCGr_G

Cuts at:      0     129    1077 
   Size:        129     948
 
AvrII C'CTAG_G

Cuts at:      0     399    1077 
   Size:        399     678
 
BanI G'GyrC_C

Cuts at:      0     404     538     658     701    1077 
   Size:        404     134     120      43     376

  Fragments arranged by size: 

                 404     376     134     120      43
 
BanII G_rGCy'C

Cuts at:      0     350     577    1077 
   Size:        350     227     500

  Fragments arranged by size: 

                 500     350     227
 
BbeI G_GCGC'C

Cuts at:      0     662    1077 
   Size:        662     415
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     171     917    1077 
   Size:        171     746     160

  Fragments arranged by size: 

                 746     171     160
 
BbvCI CC'TCA_GC

Cuts at:      0     764    1077 
   Size:        764     313
 
BglI GCCn_nnn'nGGC

Cuts at:      0      95    1077 
   Size:         95     982
 
BglII A'GATC_T

Cuts at:      0     979    1077 
   Size:        979      98
 
Bme1580I G_kGCm'C

Cuts at:      0     407    1077 
   Size:        407     670
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     591    1077 
   Size:        591     486
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     594     793     968    1077 
   Size:        403     191     199     175     109

  Fragments arranged by size: 

                 403     199     191     175     109
 
Bpu10I CC'TnA_GC

Cuts at:      0     235     764    1077 
   Size:        235     529     313

  Fragments arranged by size: 

                 529     313     235
 
BsaBI GATnn'nnATC

Cuts at:      0     933     967    1077 
   Size:        933      34     110

  Fragments arranged by size: 

                 933     110      34
 
BsaHI Gr'CG_yC

Cuts at:      0     153     659    1077 
   Size:        153     506     418

  Fragments arranged by size: 

                 506     418     153
 
BsaWI w'CCGG_w

Cuts at:      0     269    1077 
   Size:        269     808
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     350     380     694     724    1077 
   Size:        350      30     314      30     353

  Fragments arranged by size: 

                 353     350     314      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     342     583     790    1077 
   Size:        342     241     207     287

  Fragments arranged by size: 

                 342     287     241     207
 
BseYI C'CCAG_C

Cuts at:      0     135     219     588    1077 
   Size:        135      84     369     489

  Fragments arranged by size: 

                 489     369     135      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     699    1065    1077 
   Size:        544     155     366      12

  Fragments arranged by size: 

                 544     366     155      12
 
BsiHKAI G_wGCw'C

Cuts at:      0      77     148     605     818    1077 
   Size:         77      71     457     213     259

  Fragments arranged by size: 

                 457     259     213      77      71
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     148     350     407     577     605     818    1077 
   Size:         77      71     202      57     170      28     213     259

  Fragments arranged by size: 

                 259     213     202     170      77      71      57      28
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     110    1077 
   Size:        110     967
 
BsrFI r'CCGG_y

Cuts at:      0      85     686    1040    1077 
   Size:         85     601     354      37

  Fragments arranged by size: 

                 601     354      85      37
 
BssHII G'CGCG_C

Cuts at:      0     124     491     738    1077 
   Size:        124     367     247     339

  Fragments arranged by size: 

                 367     339     247     124
 
BssSI C'ACGA_G

Cuts at:      0     565    1077 
   Size:        565     512
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     545    1077 
   Size:        545     532
 
BstEII G'GTnAC_C

Cuts at:      0     207    1077 
   Size:        207     870
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      39     381     618    1062    1077 
   Size:         39     342     237     444      15

  Fragments arranged by size: 

                 444     342     237      39      15
 
BstYI r'GATC_y

Cuts at:      0     979    1077 
   Size:        979      98
 
BtgI C'CryG_G

Cuts at:      0     374    1077 
   Size:        374     703
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     694    1077 
   Size:        694     383
 
BtsI GCAGTG_nn'

Cuts at:      0     157     358    1077 
   Size:        157     201     719

  Fragments arranged by size: 

                 719     201     157
 
DraIII CAC_nnn'GTG

Cuts at:      0     545     827    1077 
   Size:        545     282     250

  Fragments arranged by size: 

                 545     282     250
 
EaeI y'GGCC_r

Cuts at:      0     371    1077 
   Size:        371     706
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     823    1077 
   Size:         82     741     254

  Fragments arranged by size: 

                 741     254      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     403     594     793     920     968    1077 
   Size:         63     340     191     199     127      48     109

  Fragments arranged by size: 

                 340     199     191     127     109      63      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     717    1077 
   Size:        717     360
 
EcoO109I rG'GnC_Cy

Cuts at:      0     893    1077 
   Size:        893     184
 
FspI TGC'GCA

Cuts at:      0     667    1077 
   Size:        667     410
 
HaeII r_GCGC'y

Cuts at:      0     473     625     662     805    1077 
   Size:        473     152      37     143     272

  Fragments arranged by size: 

                 473     272     152     143      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     413     445    1077 
   Size:        413      32     632

  Fragments arranged by size: 

                 632     413      32
 
KasI G'GCGC_C

Cuts at:      0     658    1077 
   Size:        658     419
 
MfeI C'AATT_G

Cuts at:      0     947    1077 
   Size:        947     130
 
MscI TGG'CCA

Cuts at:      0     373    1077 
   Size:        373     704
 
MslI CAynn'nnrTG

Cuts at:      0      37     190     384    1060    1077 
   Size:         37     153     194     676      17

  Fragments arranged by size: 

                 676     194     153      37      17
 
MspA1I CmG'CkG

Cuts at:      0      20     168     699    1077 
   Size:         20     148     531     378

  Fragments arranged by size: 

                 531     378     148      20
 
NarI GG'CG_CC

Cuts at:      0     659    1077 
   Size:        659     418
 
NcoI C'CATG_G

Cuts at:      0     374    1077 
   Size:        374     703
 
NspI r_CATG'y

Cuts at:      0     189    1077 
   Size:        189     888
 
PasI CC'CwG_GG

Cuts at:      0     111    1077 
   Size:        111     966
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51    1077 
   Size:         19      32    1026

  Fragments arranged by size: 

                1026      32      19
 
PpuMI rG'GwC_Cy

Cuts at:      0     893    1077 
   Size:        893     184
 
PstI C_TGCA'G

Cuts at:      0     698    1077 
   Size:        698     379
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82     823    1077 
   Size:         82     741     254

  Fragments arranged by size: 

                 741     254      82
 
ScaI AGT'ACT

Cuts at:      0     529    1077 
   Size:        529     548
 
SexAI A'CCwGG_T

Cuts at:      0     541    1077 
   Size:        541     536
 
SfcI C'TryA_G

Cuts at:      0     694    1077 
   Size:        694     383
 
SfoI GGC'GCC

Cuts at:      0     660    1077 
   Size:        660     417
 
SgrAI Cr'CCGG_yG

Cuts at:      0     686    1077 
   Size:        686     391
 
StyI C'CwwG_G

Cuts at:      0     374     399     958    1077 
   Size:        374      25     559     119

  Fragments arranged by size: 

                 559     374     119      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     165    1077 
   Size:        165     912
 
TatI w'GTAC_w

Cuts at:      0     527    1077 
   Size:        527     550
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     350     382     694     726    1077 
   Size:        350      32     312      32     351

  Fragments arranged by size: 

                 351     350     312      32      32
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     AvaI    AvrII     BanI    BanII
     BbeI     BbsI    BbvCI     BglI    BglII Bme1580I     BplI     BpmI
   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI     MfeI
     MscI     MslI   MspA1I     NarI     NcoI     NspI     PasI     PpiI
    PpuMI     PstI     SapI     ScaI    SexAI     SfcI     SfoI    SgrAI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI     BlpI
    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BstBI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MluI     MmeI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     SfiI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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