Restriction map for pF1KB8955
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BcnI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BseAI       BseRI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HindIII     KasI        KpnI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          301  GTCGAC               GT^[AC][GT]AC
AcyI          117  GACGCC               G[GA]^CG[CT]C
AlwI          687  GATCC                GGATC(4/5)
BcgI          104  CGAACACTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          154  GCCGACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         611  AGATCT               A^GATCT
BsaAI          28  TACGTG               [CT]AC^GT[GA]
BsaHI         117  GACGCC               G[GA]^CG[CT]C
BsaWI         875  ACCGGA               [AT]^CCGG[AT]
BsiEI         152  CGGCCG               CG[GA][CT]^CG
BsrBI         328  CCGCTC               CCGCTC(-3/-3)
BsrI          705  ACTGG                ACTGG(1/-1)
BstEII        740  GGTTACC              G^GT[ACGT]ACC
BstPI         740  GGTTACC              G^GT[ACGT]ACC
EagI          152  CGGCCG               C^GGCCG
EclXI         152  CGGCCG               C^GGCCG
Eco52I        152  CGGCCG               C^GGCCG
EcoO65I       740  GGTTACC              G^GT[ACGT]ACC
HaeII          33  GGCGCT               [GA]GCGC^[CT]
HapII         876  CCGG                 C^CGG
Hin1I         117  GACGCC               G[GA]^CG[CT]C
HinfI         414  GACTC                G^A[ACGT]TC
HpaI          757  GTTAAC               GTT^AAC
HpaII         876  CCGG                 C^CGG
KspI           89  CCGCGG               CCGC^GG
MslI          711  CATGATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          876  CCGG                 C^CGG
PaeR7I        663  CTCGAG               C^TCGAG
PleI          414  GACTC                GAGTC(4/5)
SacI          270  GAGCTC               GAGCT^C
SacII          89  CCGCGG               CCGC^GG
SalI          301  GTCGAC               G^TCGAC
SapI          171  GCTCTTC              GCTCTTC(1/4)
SbfI          626  CCTGCAGG             CCTGCA^GG
SfaNI         404  GCATC                GCATC(5/9)
SfiI          153  GGCCGACGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          663  CTCGAG               C^T[CT][GA]AG
SphI          750  GCATGC               GCATG^C
Sse8387I      626  CCTGCAGG             CCTGCA^GG
XhoI          663  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AfaI           27  GTAC                 GT^AC
              970  GTAC                
AspHI         259  GTGCTC               G[AT]GC[AT]^C
              270  GAGCTC              
AvaI          359  CCCGAG               C^[CT]CG[GA]G
              663  CTCGAG              
BanII         169  GGGCTC               G[GA]GC[CT]^C
              270  GAGCTC              
BsgI          213  CTGCAC               GTGCAG(16/14)
              459  CTGCAC              
BsiHKAI       259  GTGCTC               G[AT]GC[AT]^C
              270  GAGCTC              
BsoBI         359  CCCGAG               C^[CT]CG[GA]G
              663  CTCGAG              
BsrDI         375  CATTGC               GCAATG(2/0)
              811  GCAATG              
BstF5I        180  CATCC                GGATG(2/0)
              474  GGATG               
BstYI         611  AGATCT               [GA]^GATC[CT]
              686  AGATCC              
DraII         355  GGGACCC              [GA]G^G[ACGT]CC[CT]
              389  GGGTCCT             
EarI          172  CTCTTC               CTCTTC(1/4)
              235  CTCTTC              
EcoO109I      355  GGGACCC              [GA]G^G[ACGT]CC[CT]
              389  GGGTCCT             
FokI          180  CATCC                GGATG(9/13)
              474  GGATG               
HgaI           19  GACGC                GACGC(5/10)
              117  GACGC               
HincII        301  GTCGAC               GT[CT]^[GA]AC
              757  GTTAAC              
HindII        301  GTCGAC               GT[CT]^[GA]AC
              757  GTTAAC              
HphI          597  GGTGA                GGTGA(8/7)
              194  TCACC               
Ksp632I       172  CTCTTC               CTCTTC(1/4)
              235  CTCTTC              
MflI          611  AGATCT               [GA]^GATC[CT]
              686  AGATCC              
MseI          608  TTAA                 T^TAA
              758  TTAA                
MspA1I         89  CCGCGG               C[AC]G^C[GT]G
              679  CAGCGG              
NspI          750  GCATGC               [GA]CATG^[CT]
              837  ACATGC              
PpuMI         355  GGGACCC              [GA]G^G[AT]CC[CT]
              389  GGGTCCT             
PstI          112  CTGCAG               CTGCA^G
              627  CTGCAG              
RsaI           27  GTAC                 GT^AC
              970  GTAC                
SfcI          112  CTGCAG               C^T[GA][CT]AG
              627  CTGCAG              
TaqI          302  TCGA                 T^CGA
              664  TCGA                
Tru9I         608  TTAA                 T^TAA
              758  TTAA                
TthHB8I       302  TCGA                 T^CGA
              664  TCGA                
XhoII         611  AGATCT               [GA]^GATC[CT]
              686  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BalI        
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      FauI        Fnu4HI      HaeIII      HhaI        
HinP1I      ItaI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        
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