Restriction map for pF1KB8955
 (Linear) (Six-base) MAPSORT of: KIBB8955.seq  Check: 2862  from: 1  to: 996



KIBB8955.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     302     996 
   Size:        302     694
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     622     996 
   Size:        264     358     374

  Fragments arranged by size: 

                 374     358     264
 
AvaI C'yCGr_G

Cuts at:      0     359     663     996 
   Size:        359     304     333

  Fragments arranged by size: 

                 359     333     304
 
BanII G_rGCy'C

Cuts at:      0     173     274     996 
   Size:        173     101     722

  Fragments arranged by size: 

                 722     173     101
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127     996 
   Size:         93      34     869

  Fragments arranged by size: 

                 869      93      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160     996 
   Size:        160     836
 
BglII A'GATC_T

Cuts at:      0     611     996 
   Size:        611     385
 
Bme1580I G_kGCm'C

Cuts at:      0     100     996 
   Size:        100     896
 
BmgBI CAC'GTC

Cuts at:      0     159     996 
   Size:        159     837
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      58     661     900     996 
   Size:         28      30     603     239      96

  Fragments arranged by size: 

                 603     239      96      30      28
 
Bpu10I CC'TnA_GC

Cuts at:      0     331     785     996 
   Size:        331     454     211

  Fragments arranged by size: 

                 454     331     211
 
BsaAI yAC'GTr

Cuts at:      0      30     996 
   Size:         30     966
 
BsaHI Gr'CG_yC

Cuts at:      0     118     996 
   Size:        118     878
 
BsaWI w'CCGG_w

Cuts at:      0     875     996 
   Size:        875     121
 
BseYI C'CCAG_C

Cuts at:      0     964     996 
   Size:        964      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     444     996 
   Size:        198     246     552

  Fragments arranged by size: 

                 552     246     198
 
BsiEI CG_ry'CG

Cuts at:      0     155     996 
   Size:        155     841
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     274     996 
   Size:        263      11     722

  Fragments arranged by size: 

                 722     263      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     173     263     274     996 
   Size:        100      73      90      11     722

  Fragments arranged by size: 

                 722     100      90      73      11
 
BsrBI CCG'CTC

Cuts at:      0     330     996 
   Size:        330     666
 
BsrDI GCAATG_nn'

Cuts at:      0     374     818     996 
   Size:        374     444     178

  Fragments arranged by size: 

                 444     374     178
 
BstEII G'GTnAC_C

Cuts at:      0     740     996 
   Size:        740     256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     204     705     717     996 
   Size:        204     501      12     279

  Fragments arranged by size: 

                 501     279     204      12
 
BstYI r'GATC_y

Cuts at:      0     611     686     996 
   Size:        611      75     310

  Fragments arranged by size: 

                 611     310      75
 
BtgI C'CryG_G

Cuts at:      0      89     164     293     996 
   Size:         89      75     129     703

  Fragments arranged by size: 

                 703     129      89      75
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      33     996 
   Size:         33     963
 
BtsI GCAGTG_nn'

Cuts at:      0     580     996 
   Size:        580     416
 
EaeI y'GGCC_r

Cuts at:      0     152     161     290     314     707     821     996 
   Size:        152       9     129      24     393     114     175

  Fragments arranged by size: 

                 393     175     152     129     114      24       9
 
EagI C'GGCC_G

Cuts at:      0     152     996 
   Size:        152     844
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     241     996 
   Size:        178      63     755

  Fragments arranged by size: 

                 755     178      63
 
EcoICRI GAG'CTC

Cuts at:      0     272     996 
   Size:        272     724
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      58     264     622     661     900     996 
   Size:         28      30     206     358      39     239      96

  Fragments arranged by size: 

                 358     239     206      96      39      30      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     356     390     996 
   Size:        356      34     606

  Fragments arranged by size: 

                 606     356      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     531     996 
   Size:        531     465
 
HaeII r_GCGC'y

Cuts at:      0      37     996 
   Size:         37     959
 
HincII GTy'rAC

Cuts at:      0     303     759     996 
   Size:        303     456     237

  Fragments arranged by size: 

                 456     303     237
 
HpaI GTT'AAC

Cuts at:      0     759     996 
   Size:        759     237
 
MscI TGG'CCA

Cuts at:      0     316     709     823     996 
   Size:        316     393     114     173

  Fragments arranged by size: 

                 393     316     173     114
 
MslI CAynn'nnrTG

Cuts at:      0     715     996 
   Size:        715     281
 
MspA1I CmG'CkG

Cuts at:      0      91     681     996 
   Size:         91     590     315

  Fragments arranged by size: 

                 590     315      91
 
NspI r_CATG'y

Cuts at:      0     754     841     996 
   Size:        754      87     155

  Fragments arranged by size: 

                 754     155      87
 
PasI CC'CwG_GG

Cuts at:      0     470     996 
   Size:        470     526
 
PpuMI rG'GwC_Cy

Cuts at:      0     356     390     996 
   Size:        356      34     606

  Fragments arranged by size: 

                 606     356      34
 
PsiI TTA'TAA

Cuts at:      0     589     996 
   Size:        589     407
 
PspXI vC'TCGA_Gb

Cuts at:      0     663     996 
   Size:        663     333
 
PstI C_TGCA'G

Cuts at:      0     116     631     996 
   Size:        116     515     365

  Fragments arranged by size: 

                 515     365     116
 
SacI G_AGCT'C

Cuts at:      0     274     996 
   Size:        274     722
 
SacII CC_GC'GG

Cuts at:      0      92     996 
   Size:         92     904
 
SalI G'TCGA_C

Cuts at:      0     301     996 
   Size:        301     695
 
SanDI GG'GwC_CC

Cuts at:      0     356     996 
   Size:        356     640
 
SapI GCTCTTCn'nnn_

Cuts at:      0     178     996 
   Size:        178     818
 
SbfI CC_TGCA'GG

Cuts at:      0     631     996 
   Size:        631     365
 
SfcI C'TryA_G

Cuts at:      0     112     627     996 
   Size:        112     515     369

  Fragments arranged by size: 

                 515     369     112
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     160     996 
   Size:        160     836
 
SmlI C'TyrA_G

Cuts at:      0     663     996 
   Size:        663     333
 
SphI G_CATG'C

Cuts at:      0     754     996 
   Size:        754     242
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     554     996 
   Size:        554     442
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132     425     540     996 
   Size:        132     293     115     456

  Fragments arranged by size: 

                 456     293     132     115
 
XhoI C'TCGA_G

Cuts at:      0     663     996 
   Size:        663     333
 

 Enzymes that do cut:

     AccI     AcuI     AvaI    BanII     BcgI     BglI    BglII Bme1580I
    BmgBI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrDI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I     FalI    HaeII   HincII     HpaI     MscI     MslI   MspA1I
     NspI     PasI    PpuMI     PsiI    PspXI     PstI     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfcI     SfiI     SmlI     SphI
    TaqII     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BlpI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI     PsrI     PvuI    PvuII    RsrII     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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