Restriction map for pF1KB8951
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfaI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeI        MamI        MluI        MroI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TfiI        TspRI       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          541  GTCTAC               GT^[AC][GT]AC
AccII         886  CGCG                 CG^CG
AflII         898  CTTAAG               C^TTAAG
AlwNI         112  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI           73  CCCGGG               C^[CT]CG[GA]G
BalI          377  TGGCCA               TGG^CCA
BamHI         395  GGATCC               G^GATCC
BanI          175  GGTGCC               G^G[CT][GA]CC
BbsI          607  GTCTTC               GAAGAC(2/6)
BbvCI         732  CCTCAGC              CCTCAGC(-5/-2)
BclI          497  TGATCA               T^GATCA
BfrI          898  CTTAAG               C^TTAAG
BglI          289  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          601  CTGGAG               CTGGAG(16/14)
BpuAI         607  GTCTTC               GAAGAC(2/6)
BsaI           63  GGTCTC               GGTCTC(1/5)
BsgI          932  CTGCAC               GTGCAG(16/14)
BsmAI          64  GTCTC                GTCTC(1/5)
BsmFI         414  GGGAC                GGGAC(10/14)
BsoBI          73  CCCGGG               C^[CT]CG[GA]G
BspMI         235  ACCTGC               ACCTGC(4/8)
BsrBI         457  GAGCGG               CCGCTC(-3/-3)
BsrDI         813  CATTGC               GCAATG(2/0)
BsrFI         385  GCCGGC               [GA]^CCGG[CT]
BstEII        354  GGTGACC              G^GT[ACGT]ACC
BstPI         354  GGTGACC              G^GT[ACGT]ACC
BstUI         886  CGCG                 CG^CG
BstXI         698  CCAAGTCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          115  GCGC                 GCG^C
Cfr10I        385  GCCGGC               [GA]^CCGG[CT]
DsaI          170  CCGTGG               C^C[GA][CT]GG
EcoO65I       354  GGTGACC              G^GT[ACGT]ACC
FbaI          497  TGATCA               T^GATCA
HaeII         114  GGCGCT               [GA]GCGC^[CT]
HgaI          887  GCGTC                GACGC(5/10)
HhaI          115  GCGC                 GCG^C
HinP1I        115  GCGC                 G^CGC
MaeII          80  ACGT                 A^CGT
MfeI          299  CAATTG               C^AATTG
MluNI         377  TGGCCA               TGG^CCA
MscI          377  TGGCCA               TGG^CCA
MseI          899  TTAA                 T^TAA
MunI          299  CAATTG               C^AATTG
MvnI          886  CGCG                 CG^CG
NaeI          385  GCCGGC               GCC^GGC
NgoMIV        385  GCCGGC               G^CCGGC
PvuII         735  CAGCTG               CAG^CTG
SacI          276  GAGCTC               GAGCT^C
ScaI          671  AGTACT               AGT^ACT
SmaI           73  CCCGGG               CCC^GGG
SmlI          898  CTTAAG               C^T[CT][GA]AG
StuI          967  AGGCCT               AGG^CCT
Tru9I         899  TTAA                 T^TAA
Tsp509I       300  AATT                 ^AATT
XcmI          473  CCAACGGCAGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          73  CCCGGG               C^CCGGG
XmaI           73  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AspHI          49  GTGCTC               G[AT]GC[AT]^C
              276  GAGCTC              
BanII         276  GAGCTC               G[GA]GC[CT]^C
              490  GAGCCC              
BsaWI         255  TCCGGT               [AT]^CCGG[AT]
              338  TCCGGT              
BseRI         580  CTCCTC               GAGGAG(10/8)
              651  CTCCTC              
BsiHKAI        49  GTGCTC               G[AT]GC[AT]^C
              276  GAGCTC              
MaeIII        355  GTGAC                ^GT[ACGT]AC
              889  GTCAC               
MslI           42  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              522  CATCAGCATG          
MspA1I        735  CAGCTG               C[AC]G^C[GT]G
              921  CCGCTG              
TaqI           44  TCGA                 T^CGA
              512  TCGA                
Tsp45I        355  GTGAC                ^GT[GC]AC
              889  GTCAC               
TthHB8I        44  TCGA                 T^CGA
              512  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        XhoII       
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