Restriction map for pF1KB8932
 (Linear) (Six-base) MAPSORT of: KIBB8932.seq  Check: 2394  from: 1  to: 849



KIBB8932.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     370     849 
   Size:        370     479
 
AflIII A'CryG_T

Cuts at:      0     383     849 
   Size:        383     466
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     475     507     849 
   Size:        475      32     342

  Fragments arranged by size: 

                 475     342      32
 
ApaI G_GGCC'C

Cuts at:      0     296     849 
   Size:        296     553
 
AvaI C'yCGr_G

Cuts at:      0     767     849 
   Size:        767      82
 
BanI G'GyrC_C

Cuts at:      0      72     154     207     409     849 
   Size:         72      82      53     202     440

  Fragments arranged by size: 

                 440     202      82      72      53
 
BanII G_rGCy'C

Cuts at:      0     296     335     849 
   Size:        296      39     514

  Fragments arranged by size: 

                 514     296      39
 
BbeI G_GCGC'C

Cuts at:      0      76     158     413     849 
   Size:         76      82     255     436

  Fragments arranged by size: 

                 436     255      82      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0     291     831     849 
   Size:        291     540      18

  Fragments arranged by size: 

                 540     291      18
 
BglII A'GATC_T

Cuts at:      0     572     849 
   Size:        572     277
 
BlpI GC'TnA_GC

Cuts at:      0     263     849 
   Size:        263     586
 
Bme1580I G_kGCm'C

Cuts at:      0     212     296     849 
   Size:        212      84     553

  Fragments arranged by size: 

                 553     212      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     346     502     798     849 
   Size:        190     156     156     296      51

  Fragments arranged by size: 

                 296     190     156     156      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     668     793     849 
   Size:        668     125      56

  Fragments arranged by size: 

                 668     125      56
 
BsaHI Gr'CG_yC

Cuts at:      0      73     155     320     410     849 
   Size:         73      82     165      90     439

  Fragments arranged by size: 

                 439     165      90      82      73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     475     505     725     755     849 
   Size:        475      30     220      30      94

  Fragments arranged by size: 

                 475     220      94      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     343     445     625     712     849 
   Size:         25     318     102     180      87     137

  Fragments arranged by size: 

                 318     180     137     102      87      25
 
BsiEI CG_ry'CG

Cuts at:      0     520     835     849 
   Size:        520     315      14

  Fragments arranged by size: 

                 520     315      14
 
BsiWI C'GTAC_G

Cuts at:      0     200     849 
   Size:        200     649
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     296     335     849 
   Size:        212      84      39     514

  Fragments arranged by size: 

                 514     212      84      39
 
BsrGI T'GTAC_A

Cuts at:      0     107     849 
   Size:        107     742
 
BssHII G'CGCG_C

Cuts at:      0     164     462     849 
   Size:        164     298     387

  Fragments arranged by size: 

                 387     298     164
 
BstYI r'GATC_y

Cuts at:      0     572     849 
   Size:        572     277
 
BtgI C'CryG_G

Cuts at:      0     230     358     849 
   Size:        230     128     491

  Fragments arranged by size: 

                 491     230     128
 
EaeI y'GGCC_r

Cuts at:      0      26     517     832     849 
   Size:         26     491     315      17

  Fragments arranged by size: 

                 491     315      26      17
 
EagI C'GGCC_G

Cuts at:      0     517     832     849 
   Size:        517     315      17

  Fragments arranged by size: 

                 517     315      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     346     502     798     849 
   Size:        190     156     156     296      51

  Fragments arranged by size: 

                 296     190     156     156      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     293     849 
   Size:         45     248     556

  Fragments arranged by size: 

                 556     248      45
 
HaeII r_GCGC'y

Cuts at:      0      76      89     158     413     712     849 
   Size:         76      13      69     255     299     137

  Fragments arranged by size: 

                 299     255     137      76      69      13
 
KasI G'GCGC_C

Cuts at:      0      72     154     409     849 
   Size:         72      82     255     440

  Fragments arranged by size: 

                 440     255      82      72
 
MluI A'CGCG_T

Cuts at:      0     383     849 
   Size:        383     466
 
MspA1I CmG'CkG

Cuts at:      0     232     348     471     640     751     849 
   Size:        232     116     123     169     111      98

  Fragments arranged by size: 

                 232     169     123     116     111      98
 
NarI GG'CG_CC

Cuts at:      0      73     155     410     849 
   Size:         73      82     255     439

  Fragments arranged by size: 

                 439     255      82      73
 
NcoI C'CATG_G

Cuts at:      0     358     849 
   Size:        358     491
 
NotI GC'GGCC_GC

Cuts at:      0     832     849 
   Size:        832      17
 
PasI CC'CwG_GG

Cuts at:      0     159     849 
   Size:        159     690
 
PfoI T'CCnGG_A

Cuts at:      0     299     323     849 
   Size:        299      24     526

  Fragments arranged by size: 

                 526     299      24
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     475     507     849 
   Size:        475      32     342

  Fragments arranged by size: 

                 475     342      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     849 
   Size:         45     804
 
PshAI GACnn'nnGTC

Cuts at:      0     640     849 
   Size:        640     209
 
PspOMI G'GGCC_C

Cuts at:      0     292     849 
   Size:        292     557
 
PvuII CAG'CTG

Cuts at:      0     348     471     640     849 
   Size:        348     123     169     209

  Fragments arranged by size: 

                 348     209     169     123
 
SacII CC_GC'GG

Cuts at:      0     233     849 
   Size:        233     616
 
ScaI AGT'ACT

Cuts at:      0      19     849 
   Size:         19     830
 
SfoI GGC'GCC

Cuts at:      0      74     156     411     849 
   Size:         74      82     255     438

  Fragments arranged by size: 

                 438     255      82      74
 
StyI C'CwwG_G

Cuts at:      0     358     849 
   Size:        358     491
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     571     849 
   Size:        571     278
 
TatI w'GTAC_w

Cuts at:      0      17     107     506     849 
   Size:         17      90     399     343

  Fragments arranged by size: 

                 399     343      90      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     725     757     849 
   Size:        725      32      92

  Fragments arranged by size: 

                 725      92      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     490     849 
   Size:        490     359
 

 Enzymes that do cut:

     AccI   AflIII     AloI     ApaI     AvaI     BanI    BanII     BbeI
     BglI    BglII     BlpI Bme1580I     BpmI   Bpu10I    BsaHI    BsaXI
    BseRI    BsiEI    BsiWI Bsp1286I    BsrGI   BssHII    BstYI     BtgI
     EaeI     EagI  Eco57MI EcoO109I    HaeII     KasI     MluI   MspA1I
     NarI     NcoI     NotI     PasI     PfoI     PpiI    PpuMI    PshAI
   PspOMI    PvuII    SacII     ScaI     SfoI     StyI    TaqII     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MmeI     MscI     MslI     NaeI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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