Restriction map for pF1KB8904
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         286  CGCG                 CG^CG
AfaI          111  GTAC                 GT^AC
AlwI           93  GATCC                GGATC(4/5)
AlwNI         595  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         654  GGACC                G^G[AT]CC
BbrPI         440  CACGTG               CAC^GTG
BbvCI         508  GCTGAGG              CCTCAGC(-5/-2)
BfaI           16  CTAG                 C^TAG
BlpI          153  GCTGAGC              GC^T[ACGT]AGC
BpmI          451  CTGGAG               CTGGAG(16/14)
Bpu1102I      153  GCTGAGC              GC^T[ACGT]AGC
BsaAI         440  CACGTG               [CT]AC^GT[GA]
BseRI          34  CTCCTC               GAGGAG(10/8)
BsgI          673  CTGCAC               GTGCAG(16/14)
BspMI          71  ACCTGC               ACCTGC(4/8)
BstEII        471  GGTGACC              G^GT[ACGT]ACC
BstPI         471  GGTGACC              G^GT[ACGT]ACC
BstUI         286  CGCG                 CG^CG
BstXI         461  CCAAGCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          92  AGATCC               [GA]^GATC[CT]
CelII         153  GCTGAGC              GC^T[ACGT]AGC
DraII         653  AGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          171  GACCAACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          535  CTCTTC               CTCTTC(1/4)
EcoNI          72  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      653  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       471  GGTGACC              G^GT[ACGT]ACC
EcoT14I        77  CCAAGG               C^C[AT][AT]GG
HaeII         612  GGCGCT               [GA]GCGC^[CT]
Ksp632I       535  CTCTTC               CTCTTC(1/4)
KspI          285  CCGCGG               CCGC^GG
MaeI           16  CTAG                 C^TAG
MaeII         441  ACGT                 A^CGT
MflI           92  AGATCC               [GA]^GATC[CT]
MslI          363  CACCAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          286  CGCG                 CG^CG
NspI          236  ACATGC               [GA]CATG^[CT]
PflMI         594  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         440  CACGTG               CAC^GTG
PmlI          440  CACGTG               CAC^GTG
PpuMI         653  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         595  CAGCTG               CAG^CTG
RsaI          111  GTAC                 GT^AC
SacII         285  CCGCGG               CCGC^GG
SfcI          616  CTACAG               C^T[GA][CT]AG
StyI           77  CCAAGG               C^C[AT][AT]GG
Tsp45I        472  GTGAC                ^GT[GC]AC
Van91I        594  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          92  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AspHI         370  GTGCTC               G[AT]GC[AT]^C
              387  GTGCTC              
BcnI           99  CCGGG                CC^[GC]GG
              215  CCCGG               
BmyI          370  GTGCTC               G[AGT]GC[ACT]^C
              387  GTGCTC              
BsiHKAI       370  GTGCTC               G[AT]GC[AT]^C
              387  GTGCTC              
BsmAI         456  GTCTC                GTCTC(1/5)
              694  GTCTC               
BsmFI         101  GGGAC                GGGAC(10/14)
              352  GTCCC               
Bsp1286I      370  GTGCTC               G[AGT]GC[ACT]^C
              387  GTGCTC              
BsrI          104  ACTGG                ACTGG(1/-1)
              200  ACTGG               
BstF5I        684  CATCC                GGATG(2/0)
              249  GGATG               
CfoI           60  GCGC                 GCG^C
              613  GCGC                
Cfr13I        309  GGCCC                G^G[ACGT]CC
              654  GGACC               
DpnII          13  GATC                 ^GATC
               93  GATC                
DsaI          285  CCGCGG               C^C[GA][CT]GG
              318  CCGTGG              
FokI          684  CATCC                GGATG(9/13)
              249  GGATG               
HapII          99  CCGG                 C^CGG
              216  CCGG                
HhaI           60  GCGC                 GCG^C
              613  GCGC                
HinP1I         60  GCGC                 G^CGC
              613  GCGC                
HpaII          99  CCGG                 C^CGG
              216  CCGG                
MaeIII        197  GTAAC                ^GT[ACGT]AC
              472  GTGAC               
MboI           13  GATC                 ^GATC
               93  GATC                
MboII          90  GAAGA                GAAGA(8/7)
              536  TCTTC               
MspA1I        285  CCGCGG               C[AC]G^C[GT]G
              595  CAGCTG              
MspI           99  CCGG                 C^CGG
              216  CCGG                
NciI           99  CCGGG                CC^[GC]GG
              215  CCCGG               
NdeII          13  GATC                 ^GATC
               93  GATC                
NlaIII        237  CATG                 CATG^
              393  CATG                
NlaIV          68  GGAACC               GG[ACGT]^[ACGT]CC
              654  GGACCC              
Sau3AI         13  GATC                 ^GATC
               93  GATC                
Sau96I        309  GGCCC                G^G[ACGT]CC
              654  GGACC               
StuI           30  AGGCCT               AGG^CCT
              296  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstNI       Cac8I       DdeI        
EcoRII      FauI        Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MnlI        MvaI        MwoI        
PleI        ScrFI       TseI        TspRI       
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