Restriction map for pF1KB8894
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BsrI        
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI          385  GTAC                 GT^AC
Alw44I         37  GTGCAC               G^TGCAC
AlwNI          96  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          37  GTGCAC               G^TGCAC
BbsI          516  GAAGAC               GAAGAC(2/6)
BpmI          637  CTGGAG               CTGGAG(16/14)
BpuAI         516  GAAGAC               GAAGAC(2/6)
BspMI         458  ACCTGC               ACCTGC(4/8)
BsrBI         322  GAGCGG               CCGCTC(-3/-3)
BsrFI         217  GCCGGC               [GA]^CCGG[CT]
BssSI          43  CACGAG               CACGAG(-5/-1)
BstF5I        375  CATCC                GGATG(2/0)
Cfr10I        217  GCCGGC               [GA]^CCGG[CT]
DdeI          335  CTCAG                C^T[ACGT]AG
DpnII         510  GATC                 ^GATC
DraIII         28  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          129  CCATGG               C^C[GA][CT]GG
EarI          537  GAAGAG               CTCTTC(1/4)
FauI          388  CCCGC                CCCGC(4/6)
FokI          375  CATCC                GGATG(9/13)
HphI          291  GGTGA                GGTGA(8/7)
Ksp632I       537  GAAGAG               CTCTTC(1/4)
MboI          510  GATC                 ^GATC
NaeI          217  GCCGGC               GCC^GGC
NcoI          129  CCATGG               C^CATGG
NdeII         510  GATC                 ^GATC
NgoMIV        217  GCCGGC               G^CCGGC
PflMI          27  CCACCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          102  CTGCAG               CTGCA^G
PvuII          77  CAGCTG               CAG^CTG
RsaI          385  GTAC                 GT^AC
SacI          640  GAGCTC               GAGCT^C
Sau3AI        510  GATC                 ^GATC
SmaI          267  CCCGGG               CCC^GGG
SmlI          643  CTCAAG               C^T[CT][GA]AG
StuI          494  AGGCCT               AGG^CCT
TspRI         575  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         27  CCACCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         267  CCCGGG               C^CCGGG
XmaI          267  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII         284  CGCG                 CG^CG
              367  CGCG                
AcyI          211  GGCGCC               G[GA]^CG[CT]C
              256  GGCGCC              
ApaI          262  GGGCCC               GGGCC^C
              632  GGGCCC              
AspHI          37  GTGCAC               G[AT]GC[AT]^C
              640  GAGCTC              
AvaII         225  GGACC                G^G[AT]CC
              456  GGACC               
BalI          428  TGGCCA               TGG^CCA
              464  TGGCCA              
BbeI          211  GGCGCC               GGCGC^C
              256  GGCGCC              
BglI          496  GCCTTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              605  GCCGGACGGGC         
BsaHI         211  GGCGCC               G[GA]^CG[CT]C
              256  GGCGCC              
BseRI         112  GAGGAG               GAGGAG(10/8)
              532  GAGGAG              
BsiHKAI        37  GTGCAC               G[AT]GC[AT]^C
              640  GAGCTC              
BstUI         284  CGCG                 CG^CG
              367  CGCG                
DraII         262  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              632  GGGCCCT             
EcoO109I      262  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              632  GGGCCCT             
EcoT14I       129  CCATGG               C^C[AT][AT]GG
              467  CCAAGG              
Hin1I         211  GGCGCC               G[GA]^CG[CT]C
              256  GGCGCC              
HinfI           3  GAGTC                G^A[ACGT]TC
              333  GACTC               
KasI          211  GGCGCC               G^GCGCC
              256  GGCGCC              
MluNI         428  TGGCCA               TGG^CCA
              464  TGGCCA              
MscI          428  TGGCCA               TGG^CCA
              464  TGGCCA              
MvnI          284  CGCG                 CG^CG
              367  CGCG                
NarI          211  GGCGCC               GG^CGCC
              256  GGCGCC              
PleI          333  GACTC                GAGTC(4/5)
                3  GAGTC               
SfaNI         341  GCATC                GCATC(5/9)
              374  GCATC               
SfoI          211  GGCGCC               GGC^GCC
              256  GGCGCC              
StyI          129  CCATGG               C^C[AT][AT]GG
              467  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AvaI        BanI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstNI       
Cac8I       CfoI        Cfr13I      EaeI        EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MaeII       MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
SfcI        TseI        
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