Restriction map for pF1KB8879
 (Linear) (Six-base) MAPSORT of: KIBB8879.seq  Check: 9461  from: 1  to: 519



KIBB8879.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AleI CACnn'nnGTG

Cuts at:      0     446     519 
   Size:        446      73
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     435     519 
   Size:        435      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0      91     519 
   Size:         91     428
 
ApaLI G'TGCA_C

Cuts at:      0     410     519 
   Size:        410     109
 
AvaI C'yCGr_G

Cuts at:      0     336     423     519 
   Size:        336      87      96

  Fragments arranged by size: 

                 336      96      87
 
BanI G'GyrC_C

Cuts at:      0      69     237     519 
   Size:         69     168     282

  Fragments arranged by size: 

                 282     168      69
 
BanII G_rGCy'C

Cuts at:      0     101     375     519 
   Size:        101     274     144

  Fragments arranged by size: 

                 274     144     101
 
BbeI G_GCGC'C

Cuts at:      0     241     519 
   Size:        241     278
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     315     519 
   Size:        315     204
 
BglI GCCn_nnn'nGGC

Cuts at:      0     110     250     341     519 
   Size:        110     140      91     178

  Fragments arranged by size: 

                 178     140     110      91
 
Bme1580I G_kGCm'C

Cuts at:      0     414     519 
   Size:        414     105
 
BmgBI CAC'GTC

Cuts at:      0     481     519 
   Size:        481      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20     519 
   Size:         20     499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     519 
   Size:        128     391
 
BsaHI Gr'CG_yC

Cuts at:      0     238     519 
   Size:        238     281
 
BsaWI w'CCGG_w

Cuts at:      0     149     519 
   Size:        149     370
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     267     297     519 
   Size:        267      30     222

  Fragments arranged by size: 

                 267     222      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288     519 
   Size:        288     231
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     440     519 
   Size:        440      79
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     414     519 
   Size:        101     313     105

  Fragments arranged by size: 

                 313     105     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     375     414     519 
   Size:        101     274      39     105

  Fragments arranged by size: 

                 274     105     101      39
 
BspEI T'CCGG_A

Cuts at:      0     149     519 
   Size:        149     370
 
BspHI T'CATG_A

Cuts at:      0     318     519 
   Size:        318     201
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     519 
   Size:        122     397
 
BssHII G'CGCG_C

Cuts at:      0     159     519 
   Size:        159     360
 
BssSI C'ACGA_G

Cuts at:      0     256     519 
   Size:        256     263
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      91     519 
   Size:         91     428
 
BstEII G'GTnAC_C

Cuts at:      0     141     519 
   Size:        141     378
 
BtgI C'CryG_G

Cuts at:      0     205     361     507     519 
   Size:        205     156     146      12

  Fragments arranged by size: 

                 205     156     146      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     501     519 
   Size:        501      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     359     519 
   Size:        359     160
 
EcoICRI GAG'CTC

Cuts at:      0      99     519 
   Size:         99     420
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     519 
   Size:        128     391
 
EcoO109I rG'GnC_Cy

Cuts at:      0      22     519 
   Size:         22     497
 
FspI TGC'GCA

Cuts at:      0     130     519 
   Size:        130     389
 
HaeII r_GCGC'y

Cuts at:      0      57     232     241     336     519 
   Size:         57     175       9      95     183

  Fragments arranged by size: 

                 183     175      95      57       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299     519 
   Size:        267      32     220

  Fragments arranged by size: 

                 267     220      32
 
KasI G'GCGC_C

Cuts at:      0     237     519 
   Size:        237     282
 
MslI CAynn'nnrTG

Cuts at:      0     323     446     519 
   Size:        323     123      73

  Fragments arranged by size: 

                 323     123      73
 
MspA1I CmG'CkG

Cuts at:      0      88     207     247     519 
   Size:         88     119      40     272

  Fragments arranged by size: 

                 272     119      88      40
 
NarI GG'CG_CC

Cuts at:      0     238     519 
   Size:        238     281
 
PfoI T'CCnGG_A

Cuts at:      0     435     519 
   Size:        435      84
 
PpuMI rG'GwC_Cy

Cuts at:      0      22     519 
   Size:         22     497
 
PstI C_TGCA'G

Cuts at:      0      35     519 
   Size:         35     484
 
SacI G_AGCT'C

Cuts at:      0     101     519 
   Size:        101     418
 
SacII CC_GC'GG

Cuts at:      0     208     519 
   Size:        208     311
 
SanDI GG'GwC_CC

Cuts at:      0      22     519 
   Size:         22     497
 
SexAI A'CCwGG_T

Cuts at:      0     312     519 
   Size:        312     207
 
SfcI C'TryA_G

Cuts at:      0      31     519 
   Size:         31     488
 
SfoI GGC'GCC

Cuts at:      0     239     519 
   Size:        239     280
 
SmaI CCC'GGG

Cuts at:      0     425     519 
   Size:        425      94
 
Tth111I GACn'n_nGTC

Cuts at:      0     314     519 
   Size:        314     205
 
XmaI C'CCGG_G

Cuts at:      0     423     519 
   Size:        423      96
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI    ApaLI     AvaI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I    BmgBI     BmrI     BpmI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
   BssHII    BssSI   BstAPI   BstEII     BtgI    BtgZI     EciI  EcoICRI
  Eco57MI EcoO109I     FspI    HaeII    Hin4I     KasI     MslI   MspA1I
     NarI     PfoI    PpuMI     PstI     SacI    SacII    SanDI    SexAI
     SfcI     SfoI     SmaI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI
    BsrDI    BsrFI    BsrGI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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