Restriction map for pF1KB8876
 (Linear) (Six-base) MAPSORT of: KIBB8876.seq  Check: 246  from: 1  to: 501



KIBB8876.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     242     501 
   Size:        242     259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     501 
   Size:        395     106
 
AgeI A'CCGG_T

Cuts at:      0     172     501 
   Size:        172     329
 
AleI CACnn'nnGTG

Cuts at:      0     427     501 
   Size:        427      74
 
AlwNI CAG_nnn'CTG

Cuts at:      0     234     501 
   Size:        234     267
 
BanII G_rGCy'C

Cuts at:      0     197     501 
   Size:        197     304
 
BbvCI CC'TCA_GC

Cuts at:      0     487     501 
   Size:        487      14
 
BfrBI ATG'CAT

Cuts at:      0       3     501 
   Size:          3     498
 
Bme1580I G_kGCm'C

Cuts at:      0      68     501 
   Size:         68     433
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     394     501 
   Size:        322      72     107

  Fragments arranged by size: 

                 322     107      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     439     487     501 
   Size:        439      48      14

  Fragments arranged by size: 

                 439      48      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     370     461     501 
   Size:        370      91      40

  Fragments arranged by size: 

                 370      91      40
 
BsaAI yAC'GTr

Cuts at:      0     327     501 
   Size:        327     174
 
BsaWI w'CCGG_w

Cuts at:      0     172     501 
   Size:        172     329
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     144     501 
   Size:        144     357
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     197     501 
   Size:         68     129     304

  Fragments arranged by size: 

                 304     129      68
 
BsrFI r'CCGG_y

Cuts at:      0     172     501 
   Size:        172     329
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     468     501 
   Size:        468      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     300     372     429     501 
   Size:        300      72      57      72

  Fragments arranged by size: 

                 300      72      72      57
 
BstYI r'GATC_y

Cuts at:      0     251     315     501 
   Size:        251      64     186

  Fragments arranged by size: 

                 251     186      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     394     395     501 
   Size:        322      72       1     106

  Fragments arranged by size: 

                 322     106      72       1
 
HindIII A'AGCT_T

Cuts at:      0     407     501 
   Size:        407      94
 
MfeI C'AATT_G

Cuts at:      0     110     501 
   Size:        110     391
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     501 
   Size:        317     184
 
MslI CAynn'nnrTG

Cuts at:      0     262     427     501 
   Size:        262     165      74

  Fragments arranged by size: 

                 262     165      74
 
MspA1I CmG'CkG

Cuts at:      0     480     501 
   Size:        480      21
 
NsiI A_TGCA'T

Cuts at:      0       5     501 
   Size:          5     496
 
NspI r_CATG'y

Cuts at:      0     471     501 
   Size:        471      30
 
PasI CC'CwG_GG

Cuts at:      0     359     493     501 
   Size:        359     134       8

  Fragments arranged by size: 

                 359     134       8
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234     501 
   Size:        234     267
 
PfoI T'CCnGG_A

Cuts at:      0     311     401     501 
   Size:        311      90     100

  Fragments arranged by size: 

                 311     100      90
 
PmlI CAC'GTG

Cuts at:      0     327     501 
   Size:        327     174
 
PshAI GACnn'nnGTC

Cuts at:      0     112     501 
   Size:        112     389
 
PstI C_TGCA'G

Cuts at:      0     452     464     501 
   Size:        452      12      37

  Fragments arranged by size: 

                 452      37      12
 
PvuII CAG'CTG

Cuts at:      0     480     501 
   Size:        480      21
 
SfcI C'TryA_G

Cuts at:      0     448     460     501 
   Size:        448      12      41

  Fragments arranged by size: 

                 448      41      12
 
StyI C'CwwG_G

Cuts at:      0     380     501 
   Size:        380     121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     485     501 
   Size:        485      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     457     489     501 
   Size:        457      32      12

  Fragments arranged by size: 

                 457      32      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     429     501 
   Size:        429      72
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AleI    AlwNI    BanII    BbvCI    BfrBI
 Bme1580I     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BseRI Bsp1286I
    BsrFI   BstAPI    BstXI    BstYI  Eco57MI  HindIII     MfeI     MmeI
     MslI   MspA1I     NsiI     NspI     PasI    PflMI     PfoI     PmlI
    PshAI     PstI    PvuII     SfcI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com