Restriction map for pF1KB8846
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BamHI       BbeI        BbsI        BcgI        
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsgI        BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BsrFI       BsrGI       
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstXI       CelII       Cfr10I      ClaI        CpoI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HaeII       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII        512  TCCGGA               T^CCGGA
AcsI         1032  GAATTT               [GA]^AATT[CT]
AcyI          508  GGCGTC               G[GA]^CG[CT]C
AhdI          529  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          649  CCTCAGG              CC^T[ACGT]AGG
ApoI         1032  GAATTT               [GA]^AATT[CT]
Asp700I       516  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         529  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          152  TGGCCA               TGG^CCA
BanI          871  GGCACC               G^G[CT][GA]CC
BbvCI         320  CCTCAGC              CCTCAGC(-5/-2)
BciVI         693  GTATCC               GTATCC(6/5)
BcnI         1475  CCCGG                CC^[GC]GG
BglII         833  AGATCT               A^GATCT
BpmI          532  CTCCAG               CTGGAG(16/14)
BsaHI         508  GGCGTC               G[GA]^CG[CT]C
BsaWI         512  TCCGGA               [AT]^CCGG[AT]
BseAI         512  TCCGGA               T^CCGGA
BsiEI        1239  CGACCG               CG[GA][CT]^CG
BsmI         1377  GCATTC               GAATGC(1/-1)
BspEI         512  TCCGGA               T^CCGGA
BspLU11I      621  ACATGT               A^CATGT
BsrBI         902  GAGCGG               CCGCTC(-3/-3)
BsrDI         935  CATTGC               GCAATG(2/0)
BssHII        777  GCGCGC               G^CGCGC
Bsu36I        649  CCTCAGG              CC^T[ACGT]AGG
DraI          337  TTTAAA               TTT^AAA
DraII         812  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          814  GACCTAGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          152  TGGCCA               [CT]^GGCC[GA]
Eam1105I      529  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        649  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      812  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         163  GATATC               GAT^ATC
HgaI          509  GCGTC                GACGC(5/10)
Hin1I         508  GGCGTC               G[GA]^CG[CT]C
HindIII      1254  AAGCTT               A^AGCTT
MluNI         152  TGGCCA               TGG^CCA
MroI          512  TCCGGA               T^CCGGA
MscI          152  TGGCCA               TGG^CCA
MspA1I        156  CAGCTG               C[AC]G^C[GT]G
NciI         1475  CCCGG                CC^[GC]GG
NspI          621  ACATGT               [GA]CATG^[CT]
PpuMI         812  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1349  GACATACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         156  CAGCTG               CAG^CTG
SfcI          393  CTACAG               C^T[GA][CT]AG
SfiI          410  GGCCTCTGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI          467  AGGCCT               AGG^CCT
TfiI          118  GATTC                G^A[AT]TC
XcmI         1162  CCAATCACACGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          516  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         402  CGCG                 CG^CG
              778  CGCG                
AflIII        621  ACATGT               A^C[GA][CT]GT
             1168  ACACGT              
AlwNI        1501  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1552  CAGAAACTG           
BbrPI         546  CACGTG               CAC^GTG
             1003  CACGTG              
BglI          411  GCCTCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1000  GCCCACGTGGC         
BsaAI         546  CACGTG               [CT]AC^GT[GA]
             1003  CACGTG              
Bst1107I      666  GTATAC               GTA^TAC
             1530  GTATAC              
BstUI         402  CGCG                 CG^CG
              778  CGCG                
BstYI         833  AGATCT               [GA]^GATC[CT]
             1435  GGATCT              
BstZ17I       666  GTATAC               GTA^TAC
             1530  GTATAC              
EcoNI         697  CCTTTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1113  CCTTACAGAGG         
HapII         513  CCGG                 C^CGG
             1476  CCGG                
HpaII         513  CCGG                 C^CGG
             1476  CCGG                
MflI          833  AGATCT               [GA]^GATC[CT]
             1435  GGATCT              
MspI          513  CCGG                 C^CGG
             1476  CCGG                
MvnI          402  CGCG                 CG^CG
              778  CGCG                
NheI           93  GCTAGC               G^CTAGC
             1191  GCTAGC              
PmaCI         546  CACGTG               CAC^GTG
             1003  CACGTG              
PmlI          546  CACGTG               CAC^GTG
             1003  CACGTG              
SapI          573  GAAGAGC              GCTCTTC(1/4)
              731  GCTCTTC             
SfaNI         443  GCATC                GCATC(5/9)
              603  GCATC               
SmlI          764  CTCAAG               C^T[CT][GA]AG
             1257  CTTGAG              
XhoII         833  AGATCT               [GA]^GATC[CT]
             1435  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbvI        
BfaI        BmyI        BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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