Restriction map for pF1KB8846
 (Circular) (Six-base) MAPSORT of: pF1KB8846.seq  Check: 2538  from: 1  to: 4718



pF1KB8846  4718 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1584    1584 
   Size:       4718
 
AccI GT'mk_AC

Cuts at:    775     914    1639    1756     775 
   Size:        139     725     117    3737

  Fragments arranged by size: 

                3737     725     139     117
 
Acc65I G'GTAC_C

Cuts at:   1734    1734 
   Size:       4718
 
AclI AA'CG_TT

Cuts at:   4558    4558 
   Size:       4718
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    700    1068    2543    2975    3722    3990     700 
   Size:        368    1475     432     747     268    1428

  Fragments arranged by size: 

                1475    1428     747     432     368     268
 
AflIII A'CryG_T

Cuts at:    729    1276    2070    3174    4151     729 
   Size:        547     794    1104     977    1296

  Fragments arranged by size: 

                1296    1104     977     794     547
 
AgeI A'CCGG_T

Cuts at:   3106    3106 
   Size:       4718
 
AhdI GACnn_n'nnGTC

Cuts at:    642     642 
   Size:       4718
 
AlwNI CAG_nnn'CTG

Cuts at:   1614    1665    2023    3590    1614 
   Size:         51     358    1567    2742

  Fragments arranged by size: 

                2742    1567     358      51
 
ApaLI G'TGCA_C

Cuts at:   3488    3488 
   Size:       4718
 
ApoI r'AATT_y

Cuts at:   1140    1722    4071    4327    1140 
   Size:        582    2349     256    1531

  Fragments arranged by size: 

                2349    1531     582     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4718
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4718
 
AvaI C'yCGr_G

Cuts at:    597     895    1005    1738     597 
   Size:        298     110     733    3577

  Fragments arranged by size: 

                3577     733     298     110
 
BamHI G'GATC_C

Cuts at:   1743    1743 
   Size:       4718
 
BanI G'GyrC_C

Cuts at:    979    1734    2148    2396    2431     979 
   Size:        755     414     248      35    3266

  Fragments arranged by size: 

                3266     755     414     248      35
 
BanII G_rGCy'C

Cuts at:    400     462     688    1006    1732    2762     400 
   Size:         62     226     318     726    1030    2356

  Fragments arranged by size: 

                2356    1030     726     318     226      62
 
BbeI G_GCGC'C

Cuts at:   2152    2400    2152 
   Size:        248    4470
 
BbvCI CC'TCA_GC

Cuts at:    429     429 
   Size:       4718
 
BciVI GTATCCnnnnn_n'

Cuts at:    812    2616    3377    4718     812 
   Size:       1804     761    1341     812

  Fragments arranged by size: 

                1804    1341     812     761
 
BclI T'GATC_A

Cuts at:   2238    2238 
   Size:       4718
 
BglI GCCn_nnn'nGGC

Cuts at:    525    1114     525 
   Size:        589    4129
 
BglII A'GATC_T

Cuts at:    941    2233    4270     941 
   Size:       1292    2037    1389

  Fragments arranged by size: 

                2037    1389    1292
 
BlpI GC'TnA_GC

Cuts at:   1830    1830 
   Size:       4718
 
Bme1580I G_kGCm'C

Cuts at:    385    2343    2436    3492     385 
   Size:       1958      93    1056    1611

  Fragments arranged by size: 

                1958    1611    1056      93
 
BmgBI CAC'GTC

Cuts at:    453     482     453 
   Size:         29    4689
 
BmrI ACTGGGnnnn_n'

Cuts at:   1272    1570    1930    2011    2346    4490    1272 
   Size:        298     360      81     335    2144    1500

  Fragments arranged by size: 

                2144    1500     360     335     298      81
 
BmtI G_CTAG'C

Cuts at:    205    1303     205 
   Size:       1098    3620
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3085    3085 
   Size:       4718
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     625 
   Size:       4718
 
Bpu10I CC'TnA_GC

Cuts at:    429     429 
   Size:       4718
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    857    1386    1894    2773    3265    3563     857 
   Size:        529     508     879     492     298    2012

  Fragments arranged by size: 

                2012     879     529     508     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    538     643     698    1535    4422     538 
   Size:        105      55     837    2887     834

  Fragments arranged by size: 

                2887     837     834     105      55
 
BsaAI yAC'GTr

Cuts at:    656    1113    2071     656 
   Size:        457     958    3303

  Fragments arranged by size: 

                3303     958     457
 
BsaBI GATnn'nnATC

Cuts at:      6    2232    4269       6 
   Size:       2226    2037     455

  Fragments arranged by size: 

                2226    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    617    2149    2397    3099    4595     617 
   Size:       1532     248     702    1496     740

  Fragments arranged by size: 

                1532    1496     740     702     248
 
BsaWI w'CCGG_w

Cuts at:    620    1907    2428    3106    3380    3527    3938     620 
   Size:       1287     521     678     274     147     411    1400

  Fragments arranged by size: 

                1400    1287     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    403     433     813     843     403 
   Size:         30     380      30    4278

  Fragments arranged by size: 

                4278     380      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    411     414     417     556     860    1243     411 
   Size:          3       3     139     304     383    3886

  Fragments arranged by size: 

                3886     383     304     139       3       3
 
BseYI C'CCAG_C

Cuts at:   2143    2901    3478    3974    2143 
   Size:        758     577     496    2887

  Fragments arranged by size: 

                2887     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1350    2260    2306    3514     104 
   Size:       1246     910      46    1208    1308

  Fragments arranged by size: 

                1308    1246    1208     910      46
 
BsiHKAI G_wGCw'C

Cuts at:    400     462     688    1412    1732    2510    2700    3492     400 
   Size:         62     226     724     320     778     190     792    1626

  Fragments arranged by size: 

                1626     792     778     724     320     226     190      62
            
 
BsmI GAATG_Cn'

Cuts at:   1485    1485 
   Size:       4718
 
Bsp1286I G_dGCh'C

Cuts at:    385     400     462     688    1006    1412    1732    2343    2436 
   Size:         15      62     226     318     406     320     611      93

Cuts at:   2436    2510    2700    2762    3492     385 
   Size:         74     190      62     730    1611

  Fragments arranged by size: 

                1611     730     611     406     320     318     226     190
                  93      74      62      62      15
 
BspEI T'CCGG_A

Cuts at:    620    3938     620 
   Size:       3318    1400
 
BspHI T'CATG_A

Cuts at:   3894    3894 
   Size:       4718
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    556     716    1584    1768    1982    2284    2665     556 
   Size:        160     868     184     214     302     381    2609

  Fragments arranged by size: 

                2609     868     381     302     214     184     160
 
BsrBI CCG'CTC

Cuts at:     50    1012    3010    3064    4548    4713      50 
   Size:        962    1998      54    1484     165      55

  Fragments arranged by size: 

                1998    1484     962     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1042    2630    1042 
   Size:       1588    3130
 
BsrFI r'CCGG_y

Cuts at:   2716    2897    3106    2716 
   Size:        181     209    4328

  Fragments arranged by size: 

                4328     209     181
 
BssHII G'CGCG_C

Cuts at:    885     885 
   Size:       4718
 
BssSI C'ACGA_G

Cuts at:   2989    3347    2989 
   Size:        358    4360
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2095    2095 
   Size:       4718
 
BstBI TT'CG_AA

Cuts at:   3079    3079 
   Size:       4718
 
BstYI r'GATC_y

Cuts at:      1     941    1543    1743    2210    2233    2568    2814    3826 
   Size:        940     602     200     467      23     335     246    1012

Cuts at:   3826    3912    3924    4270       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     940     602     467     449     346     335     246
                 200      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    776    1640     776 
   Size:        864    3854
 
Bsu36I CC'TnA_GG

Cuts at:    758     758 
   Size:       4718
 
BtgI C'CryG_G

Cuts at:    514     848     983    1205    1380     514 
   Size:        334     135     222     175    3852

  Fragments arranged by size: 

                3852     334     222     175     135
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    182    2668    2849    3012     182 
   Size:       2486     181     163    1888

  Fragments arranged by size: 

                2486    1888     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1961    1961 
   Size:       4718
 
DraI TTT'AAA

Cuts at:    447    1717     447 
   Size:       1270    3448
 
DrdI GACnn_nn'nnGTC

Cuts at:    928    2424    3282     928 
   Size:       1496     858    2364

  Fragments arranged by size: 

                2364    1496     858
 
EaeI y'GGCC_r

Cuts at:    260    2303    2477    2868    2895    4240     260 
   Size:       2043     174     391      27    1345     738

  Fragments arranged by size: 

                2043    1345     738     391     174      27
 
EagI C'GGCC_G

Cuts at:   2303    2303 
   Size:       4718
 
EarI CTCTTCn'nnn_

Cuts at:    594     676     846    2741    2951     594 
   Size:         82     170    1895     210    2361

  Fragments arranged by size: 

                2361    1895     210     170      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    311    2926    3236    3382    4528     311 
   Size:       2615     310     146    1146     501

  Fragments arranged by size: 

                2615    1146     501     310     146
 
EcoICRI GAG'CTC

Cuts at:    398     460     686    1730     398 
   Size:         62     226    1044    3386

  Fragments arranged by size: 

                3386    1044     226      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     700    1068    2543    2975    3722    3990     625 
   Size:         75     368    1475     432     747     268    1353

  Fragments arranged by size: 

                1475    1353     747     432     368     268      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:    809    1225     809 
   Size:        416    4302
 
EcoO109I rG'GnC_Cy

Cuts at:    921    1857     921 
   Size:        936    3782
 
EcoRI G'AATT_C

Cuts at:   1722    1722 
   Size:       4718
 
EcoRV GAT'ATC

Cuts at:    273     273 
   Size:       4718
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2737    2737 
   Size:       4718
 
FspI TGC'GCA

Cuts at:   2499    2499 
   Size:       4718
 
HaeII r_GCGC'y

Cuts at:   2152    2400    3422    4401    2152 
   Size:        248    1022     979    2469

  Fragments arranged by size: 

                2469    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    403     435     628     660     813     845     999    1031    1138 
   Size:         32     193      32     153      32     154      32     107

Cuts at:   1138    1170    2560    2590    2592    2622     403 
   Size:         32    1390      30       2      30    2499

  Fragments arranged by size: 

                2499    1390     193     154     153     107      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1757    1757 
   Size:       4718
 
HindIII A'AGCT_T

Cuts at:   1362    1362 
   Size:       4718
 
KasI G'GCGC_C

Cuts at:   2148    2396    2148 
   Size:        248    4470
 
KpnI G_GTAC'C

Cuts at:   1738    1738 
   Size:       4718
 
MluI A'CGCG_T

Cuts at:   4151    4151 
   Size:       4718
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3389    3573    3389 
   Size:        184    4534
 
MscI TGG'CCA

Cuts at:    262    2479     262 
   Size:       2217    2501
 
MslI CAynn'nnrTG

Cuts at:    556     722    1022    1210    1415    1594    2707    2834     556 
   Size:        166     300     188     205     179    1113     127    2440

  Fragments arranged by size: 

                2440    1113     300     205     188     179     166     127
            
 
MspA1I CmG'CkG

Cuts at:    266    1829    2143    2503    3516    3761     266 
   Size:       1563     314     360    1013     245    1223

  Fragments arranged by size: 

                1563    1223    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2899    2899 
   Size:       4718
 
NarI GG'CG_CC

Cuts at:   2149    2397    2149 
   Size:        248    4470
 
NcoI C'CATG_G

Cuts at:    848    1205    1380     848 
   Size:        357     175    4186

  Fragments arranged by size: 

                4186     357     175
 
NgoMIV G'CCGG_C

Cuts at:   2897    2897 
   Size:       4718
 
NheI G'CTAG_C

Cuts at:    201    1299     201 
   Size:       1098    3620
 
NspI r_CATG'y

Cuts at:    733    1771    2271    3178     733 
   Size:       1038     500     907    2273

  Fragments arranged by size: 

                2273    1038     907     500
 
PciI A'CATG_T

Cuts at:    729    3174     729 
   Size:       2445    2273
 
PmeI GTTT'AAAC

Cuts at:   1717    1717 
   Size:       4718
 
PmlI CAC'GTG

Cuts at:    656    1113     656 
   Size:        457    4261
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3857    3889    3857 
   Size:         32    4686
 
PpuMI rG'GwC_Cy

Cuts at:    921     921 
   Size:       4718
 
PshAI GACnn'nnGTC

Cuts at:   1461    1461 
   Size:       4718
 
PstI C_TGCA'G

Cuts at:   1765    2450    1765 
   Size:        685    4033
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4718
 
PvuII CAG'CTG

Cuts at:    266    2143    2503     266 
   Size:       1877     360    2481

  Fragments arranged by size: 

                2481    1877     360
 
RsrII CG'GwC_CG

Cuts at:   2913    2913 
   Size:       4718
 
SacI G_AGCT'C

Cuts at:    400     462     688    1732     400 
   Size:         62     226    1044    3386

  Fragments arranged by size: 

                3386    1044     226      62
 
SalI G'TCGA_C

Cuts at:   1755    1755 
   Size:       4718
 
SapI GCTCTTCn'nnn_

Cuts at:    676     846    2741    2951     676 
   Size:        170    1895     210    2443

  Fragments arranged by size: 

                2443    1895     210     170
 
SbfI CC_TGCA'GG

Cuts at:   1765    1765 
   Size:       4718
 
ScaI AGT'ACT

Cuts at:   4235    4235 
   Size:       4718
 
SfcI C'TryA_G

Cuts at:     33     501    1761    2446    3439    3630      33 
   Size:        468    1260     685     993     191    1121

  Fragments arranged by size: 

                1260    1121     993     685     468     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    525     525 
   Size:       4718
 
SfoI GGC'GCC

Cuts at:   2150    2398    2150 
   Size:        248    4470
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4718
 
SmaI CCC'GGG

Cuts at:   1740    1740 
   Size:       4718
 
SmlI C'TyrA_G

Cuts at:    872    1365    1873    2788    3280    3542     872 
   Size:        493     508     915     492     262    2048

  Fragments arranged by size: 

                2048     915     508     493     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4718
 
SphI G_CATG'C

Cuts at:   1771    2271    1771 
   Size:        500    4218
 
StuI AGG'CCT

Cuts at:    577     577 
   Size:       4718
 
StyI C'CwwG_G

Cuts at:    578     848    1205    1380    1852    2206     578 
   Size:        270     357     175     472     354    3090

  Fragments arranged by size: 

                3090     472     357     354     270     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    450    2301    2434    2893    3102     450 
   Size:       1851     133     459     209    2066

  Fragments arranged by size: 

                2066    1851     459     209     133
 
TatI w'GTAC_w

Cuts at:    186    4233     186 
   Size:       4047     671
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1071    1103    4235    4267    1071 
   Size:         32    3132      32    1522

  Fragments arranged by size: 

                3132    1522      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2515    2515 
   Size:       4718
 
XbaI T'CTAG_A

Cuts at:   1749    1749 
   Size:       4718
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1277    2213    1277 
   Size:        936    3782
 
XmaI C'CCGG_G

Cuts at:   1738    1738 
   Size:       4718
 
XmnI GAAnn'nnTTC

Cuts at:    628     628 
   Size:       4718
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI     BcgI    BfrBI     BsgI    BsiWI    BsmBI
    BsrGI   BstEII    BstXI     ClaI    CspCI   DraIII     FseI    FspAI
     HpaI     MfeI     NdeI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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