Restriction map for pF1KB8843
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvrII       BalI        BamHI       BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BsaBI       
BseAI       BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      Eam1105I    
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         863  GACGTC               GACGT^C
AccI          845  GTCGAC               GT^[AC][GT]AC
AgeI          841  ACCGGT               A^CCGGT
AlwNI         991  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       806  AGCGCT               AGC^GCT
ApaI          140  GGGCCC               GGGCC^C
AspI          848  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         105  TGCGCA               TGC^GCA
BbsI          462  GAAGAC               GAAGAC(2/6)
BbvCI         984  GCTGAGG              CCTCAGC(-5/-2)
BcgI          372  CGAGACCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1179  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1179  GCTAAGC              GC^T[ACGT]AGC
BpuAI         462  GAAGAC               GAAGAC(2/6)
BsaAI          88  TACGTG               [CT]AC^GT[GA]
BsaWI         841  ACCGGT               [AT]^CCGG[AT]
BsgI          540  CTGCAC               GTGCAG(16/14)
BsmBI        1407  GAGACG               CGTCTC(1/5)
BstXI         383  CCACACTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1433  AGATCC               [GA]^GATC[CT]
CelII        1179  GCTAAGC              GC^T[ACGT]AGC
DrdI          307  GACCCCTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1261  CGGCCG               [CT]^GGCC[GA]
EagI         1261  CGGCCG               C^GGCCG
EclXI        1261  CGGCCG               C^GGCCG
Eco47III      806  AGCGCT               AGC^GCT
Eco52I       1261  CGGCCG               C^GGCCG
FspI          105  TGCGCA               TGC^GCA
MflI         1433  AGATCC               [GA]^GATC[CT]
NaeI         1120  GCCGGC               GCC^GGC
NcoI          898  CCATGG               C^CATGG
NgoMIV       1120  GCCGGC               G^CCGGC
PflFI         848  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         348  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         841  ACCGGT               A^CCGGT
PstI          565  CTGCAG               CTGCA^G
SalI          845  GTCGAC               G^TCGAC
SapI           22  GCTCTTC              GCTCTTC(1/4)
SfaNI         103  GATGC                GCATC(5/9)
SfcI          565  CTGCAG               C^T[GA][CT]AG
SmaI          531  CCCGGG               CCC^GGG
Tth111I       848  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        348  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1433  AGATCC               [GA]^GATC[CT]
XmaCI         531  CCCGGG               C^CCGGG
XmaI          531  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          643  GAATTC               [GA]^AATT[CT]
              820  GAATTC              
AfaI          178  GTAC                 GT^AC
              413  GTAC                
AlwI         1434  GATCC                GGATC(4/5)
              800  GGATC               
AocI          449  CCTCAGG              CC^T[ACGT]AGG
              883  CCTGAGG             
ApoI          643  GAATTC               [GA]^AATT[CT]
              820  GAATTC              
AvaI          531  CCCGGG               C^[CT]CG[GA]G
             1224  CTCGGG              
BsaI          373  GAGACC               GGTCTC(1/5)
              792  GAGACC              
BsiEI         843  CGGTCG               CG[GA][CT]^CG
             1261  CGGCCG              
BsoBI         531  CCCGGG               C^[CT]CG[GA]G
             1224  CTCGGG              
BsrBI         688  CCGCTC               CCGCTC(-3/-3)
             1213  GAGCGG              
BsrFI         841  ACCGGT               [GA]^CCGG[CT]
             1120  GCCGGC              
Bsu36I        449  CCTCAGG              CC^T[ACGT]AGG
              883  CCTGAGG             
Cfr10I        841  ACCGGT               [GA]^CCGG[CT]
             1120  GCCGGC              
Eco81I        449  CCTCAGG              CC^T[ACGT]AGG
              883  CCTGAGG             
EcoRI         643  GAATTC               G^AATTC
              820  GAATTC              
EcoT14I       407  CCAAGG               C^C[AT][AT]GG
              898  CCATGG              
HgaI          394  GACGC                GACGC(5/10)
             1409  GACGC               
HincII        845  GTCGAC               GT[CT]^[GA]AC
             1104  GTTGAC              
HindII        845  GTCGAC               GT[CT]^[GA]AC
             1104  GTTGAC              
KspI          272  CCGCGG               CCGC^GG
              360  CCGCGG              
MaeIII        866  GTCAC                ^GT[ACGT]AC
              913  GTGAC               
RsaI          178  GTAC                 GT^AC
              413  GTAC                
SacII         272  CCGCGG               CCGC^GG
              360  CCGCGG              
StyI          407  CCAAGG               C^C[AT][AT]GG
              898  CCATGG              
TaqI          803  TCGA                 T^CGA
              846  TCGA                
Tsp45I        866  GTCAC                ^GT[GC]AC
              913  GTGAC               
TthHB8I       803  TCGA                 T^CGA
              846  TCGA                
XcmI         1391  CCAAGCGCAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1455  CCAGAGTCTAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       Sau3AI      
Sau96I      ScrFI       SfoI        TseI        Tsp509I     
TspRI       
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