Restriction map for pF1KB8839
 (Linear) (Six-base) MAPSORT of: KIBB8839.seq  Check: 9204  from: 1  to: 1398



KIBB8839.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1398 
   Size:       1031     367
 
ApaI G_GGCC'C

Cuts at:      0    1203    1398 
   Size:       1203     195
 
ApaLI G'TGCA_C

Cuts at:      0    1238    1398 
   Size:       1238     160
 
AvaI C'yCGr_G

Cuts at:      0     629    1139    1398 
   Size:        629     510     259

  Fragments arranged by size: 

                 629     510     259
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     354     387     993    1026    1398 
   Size:        354      33     606      33     372

  Fragments arranged by size: 

                 606     372     354      33      33
 
BamHI G'GATC_C

Cuts at:      0      91     801    1398 
   Size:         91     710     597

  Fragments arranged by size: 

                 710     597      91
 
BanI G'GyrC_C

Cuts at:      0     982    1398 
   Size:        982     416
 
BanII G_rGCy'C

Cuts at:      0     468     847    1203    1398 
   Size:        468     379     356     195

  Fragments arranged by size: 

                 468     379     356     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1037    1351    1398 
   Size:       1037     314      47

  Fragments arranged by size: 

                1037     314      47
 
BclI T'GATC_A

Cuts at:      0     596    1398 
   Size:        596     802
 
BfrBI ATG'CAT

Cuts at:      0     971    1398 
   Size:        971     427
 
Bme1580I G_kGCm'C

Cuts at:      0    1203    1242    1398 
   Size:       1203      39     156

  Fragments arranged by size: 

                1203     156      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     388    1398 
   Size:        388    1010
 
BmtI G_CTAG'C

Cuts at:      0     301    1398 
   Size:        301    1097
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     452     784    1398 
   Size:        145     307     332     614

  Fragments arranged by size: 

                 614     332     307     145
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       9     847    1121    1398 
   Size:          9     838     274     277

  Fragments arranged by size: 

                 838     277     274       9
 
BseYI C'CCAG_C

Cuts at:      0     745    1398 
   Size:        745     653
 
BsiHKAI G_wGCw'C

Cuts at:      0     770     847    1223    1242    1398 
   Size:        770      77     376      19     156

  Fragments arranged by size: 

                 770     376     156      77      19
 
Bsp1286I G_dGCh'C

Cuts at:      0     468     770     847    1203    1223    1242    1398 
   Size:        468     302      77     356      20      19     156

  Fragments arranged by size: 

                 468     356     302     156      77      20      19
 
BspHI T'CATG_A

Cuts at:      0     602    1398 
   Size:        602     796
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     409    1398 
   Size:        409     989
 
BsrDI GCAATG_nn'

Cuts at:      0     327    1335    1398 
   Size:        327    1008      63

  Fragments arranged by size: 

                1008     327      63
 
BsrGI T'GTAC_A

Cuts at:      0     236    1398 
   Size:        236    1162
 
BstEII G'GTnAC_C

Cuts at:      0     105    1398 
   Size:        105    1293
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     414     933    1398 
   Size:        414     519     465

  Fragments arranged by size: 

                 519     465     414
 
BstYI r'GATC_y

Cuts at:      0      91     801    1398 
   Size:         91     710     597

  Fragments arranged by size: 

                 710     597      91
 
BtgI C'CryG_G

Cuts at:      0    1028    1398 
   Size:       1028     370
 
BtsI GCAGTG_nn'

Cuts at:      0     756    1398 
   Size:        756     642
 
DraI TTT'AAA

Cuts at:      0    1353    1398 
   Size:       1353      45
 
DraIII CAC_nnn'GTG

Cuts at:      0    1262    1398 
   Size:       1262     136
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     313    1398 
   Size:        313    1085
 
EarI CTCTTCn'nnn_

Cuts at:      0     484    1398 
   Size:        484     914
 
EcoICRI GAG'CTC

Cuts at:      0     845    1398 
   Size:        845     553
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     452     784    1031    1398 
   Size:        145     307     332     247     367

  Fragments arranged by size: 

                 367     332     307     247     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0     274     444    1200    1398 
   Size:        274     170     756     198

  Fragments arranged by size: 

                 756     274     198     170
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1365    1398 
   Size:       1365      33
 
HaeII r_GCGC'y

Cuts at:      0    1391    1398 
   Size:       1391       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     844     876     940     972    1398 
   Size:        844      32      64      32     426

  Fragments arranged by size: 

                 844     426      64      32      32
 
HincII GTy'rAC

Cuts at:      0     150     171    1398 
   Size:        150      21    1227

  Fragments arranged by size: 

                1227     150      21
 
MfeI C'AATT_G

Cuts at:      0    1268    1398 
   Size:       1268     130
 
MslI CAynn'nnrTG

Cuts at:      0     412     607    1398 
   Size:        412     195     791

  Fragments arranged by size: 

                 791     412     195
 
MspA1I CmG'CkG

Cuts at:      0     436    1398 
   Size:        436     962
 
NheI G'CTAG_C

Cuts at:      0     297    1398 
   Size:        297    1101
 
NsiI A_TGCA'T

Cuts at:      0     973    1398 
   Size:        973     425
 
NspI r_CATG'y

Cuts at:      0     744     971    1398 
   Size:        744     227     427

  Fragments arranged by size: 

                 744     427     227
 
PasI CC'CwG_GG

Cuts at:      0     797     933    1398 
   Size:        797     136     465

  Fragments arranged by size: 

                 797     465     136
 
PpuMI rG'GwC_Cy

Cuts at:      0     274     444    1398 
   Size:        274     170     954

  Fragments arranged by size: 

                 954     274     170
 
PsiI TTA'TAA

Cuts at:      0      53    1376    1398 
   Size:         53    1323      22

  Fragments arranged by size: 

                1323      53      22
 
PspOMI G'GGCC_C

Cuts at:      0    1199    1398 
   Size:       1199     199
 
PvuII CAG'CTG

Cuts at:      0     436    1398 
   Size:        436     962
 
SacI G_AGCT'C

Cuts at:      0     847    1398 
   Size:        847     551
 
SmaI CCC'GGG

Cuts at:      0    1141    1398 
   Size:       1141     257
 
SspI AAT'ATT

Cuts at:      0    1077    1398 
   Size:       1077     321
 
TatI w'GTAC_w

Cuts at:      0     236    1398 
   Size:        236    1162
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     266     298     508     540     704     736    1232    1264 
   Size:        266      32     210      32     164      32     496      32

Cuts at:   1264    1398 
   Size:        134

  Fragments arranged by size: 

                 496     266     210     164     134      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1164    1398 
   Size:       1164     234
 
XmaI C'CCGG_G

Cuts at:      0    1139    1398 
   Size:       1139     259
 

 Enzymes that do cut:

     AcuI     ApaI    ApaLI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI     BclI    BfrBI Bme1580I     BmrI     BmtI     BpmI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstEII
    BstXI    BstYI     BtgI     BtsI     DraI   DraIII     DrdI     EarI
  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     MfeI
     MslI   MspA1I     NheI     NsiI     NspI     PasI    PpuMI     PsiI
   PspOMI    PvuII     SacI     SmaI     SspI     TatI     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI     AscI
     AseI    AsiSI    AvrII     BbeI    BbvCI     BcgI    BciVI     BglI
    BglII     BlpI    BmgBI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     EaeI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmnI     ZraI
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