Restriction map for
pF1KB8831
(Circular) (Six-base) MAPSORT of: pF1KB8831.seq Check: 2244 from: 1 to: 4448
pF1KB8831 4448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 409 409
Size: 4448
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 1167 4288 1167
Size: 3121 1327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 2273 2705 3452 3720 1151
Size: 1122 432 747 268 1879
Fragments arranged by size:
1879 1122 747 432 268
AflIII A'CryG_T
Cuts at: 1800 2904 3881 1800
Size: 1104 977 2367
Fragments arranged by size:
2367 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1753 3320 1753
Size: 1567 2881
ApaLI G'TGCA_C
Cuts at: 3218 3218
Size: 4448
ApoI r'AATT_y
Cuts at: 177 1137 1452 3801 4057 177
Size: 960 315 2349 256 568
Fragments arranged by size:
2349 960 568 315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1468 1468
Size: 4448
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 1464 1878 2126 2161 1464
Size: 414 248 35 3751
Fragments arranged by size:
3751 414 248 35
BanII G_rGCy'C
Cuts at: 122 248 1462 2492 122
Size: 126 1214 1030 2078
Fragments arranged by size:
2078 1214 1030 126
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 256 331 988 1426 256
Size: 75 657 438 3278
Fragments arranged by size:
3278 657 438 75
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 869 1968 869
Size: 1099 3349
BglII A'GATC_T
Cuts at: 1192 1963 4000 1192
Size: 771 2037 1640
Fragments arranged by size:
2037 1640 771
BlpI GC'TnA_GC
Cuts at: 682 1560 682
Size: 878 3570
Bme1580I G_kGCm'C
Cuts at: 2073 2166 3222 2073
Size: 93 1056 3299
Fragments arranged by size:
3299 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 184 1660 1741 2076 4220 184
Size: 1476 81 335 2144 412
Fragments arranged by size:
2144 1476 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 923 1047 2815 923
Size: 124 1768 2556
Fragments arranged by size:
2556 1768 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1017 989
Size: 28 4420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1624 2503 2995 3293 1004
Size: 620 879 492 298 2159
Fragments arranged by size:
2159 879 620 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 406 1879 2127 2829 4325 406
Size: 1473 248 702 1496 529
Fragments arranged by size:
1496 1473 702 529 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3110 3257 3668 1637
Size: 521 678 274 147 411 2417
Fragments arranged by size:
2417 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1873 2631 3208 3704 1873
Size: 758 577 496 2617
Fragments arranged by size:
2617 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3244 104
Size: 1886 46 1208 1308
Fragments arranged by size:
1886 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 122 1462 2240 2430 3222 122
Size: 1340 778 190 792 1348
Fragments arranged by size:
1348 1340 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 122 248 1462 2073 2166 2240 2430 2492 3222
Size: 126 1214 611 93 74 190 62 730
Cuts at: 3222 122
Size: 1348
Fragments arranged by size:
1348 1214 730 611 190 126 93 74
62
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 305 1149 3624 305
Size: 844 2475 1129
Fragments arranged by size:
2475 1129 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 680 1008 1498 1712 2014 2395 680
Size: 328 490 214 302 381 2733
Fragments arranged by size:
2733 490 381 328 302 214
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 676 2360 676
Size: 1684 2764
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstYI r'GATC_y
Cuts at: 1 1192 1473 1940 1963 2298 2544 3556 3642
Size: 1191 281 467 23 335 246 1012 86
Cuts at: 3642 3654 4000 1
Size: 12 346 449
Fragments arranged by size:
1191 1012 467 449 346 335 281 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 908 908
Size: 4448
BtgI C'CryG_G
Cuts at: 107 570 107
Size: 463 3985
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 219 1691 219
Size: 1472 2976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 882 917 1098 1133 882
Size: 35 181 35 4197
Fragments arranged by size:
4197 181 35 35
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 712 2033 2207 2598 2625 3970 712
Size: 1321 174 391 27 1345 1190
Fragments arranged by size:
1345 1321 1190 391 174 27
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 887 1083 2471 2681 887
Size: 196 1388 210 2654
Fragments arranged by size:
2654 1388 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 120 1460 120
Size: 1340 3108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1017 1151 2273 2705 3452 3720 989
Size: 28 134 1122 432 747 268 1717
Fragments arranged by size:
1717 1122 747 432 268 134 28
EcoO109I rG'GnC_Cy
Cuts at: 1104 1587 1104
Size: 483 3965
EcoRI G'AATT_C
Cuts at: 177 1452 177
Size: 1275 3173
EcoRV GAT'ATC
Cuts at: 369 369
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3152 4131 1882
Size: 248 1022 979 2199
Fragments arranged by size:
2199 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 525 557 1402 1434 2290 2320 2322 2352 525
Size: 32 845 32 856 30 2 30 2621
Fragments arranged by size:
2621 856 845 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MfeI C'AATT_G
Cuts at: 509 509
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 860 1228 3119 3303 860
Size: 368 1891 184 2005
Fragments arranged by size:
2005 1891 368 184
MscI TGG'CCA
Cuts at: 714 2209 714
Size: 1495 2953
MslI CAynn'nnrTG
Cuts at: 803 2437 2564 803
Size: 1634 127 2687
Fragments arranged by size:
2687 1634 127
MspA1I CmG'CkG
Cuts at: 1559 1873 2233 3246 3491 1559
Size: 314 360 1013 245 2516
Fragments arranged by size:
2516 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NcoI C'CATG_G
Cuts at: 107 570 107
Size: 463 3985
NdeI CA'TA_TG
Cuts at: 530 1439 530
Size: 909 3539
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 992 1501 2001 2908 992
Size: 509 500 907 2532
Fragments arranged by size:
2532 907 509 500
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 1104 1104
Size: 4448
PshAI GACnn'nnGTC
Cuts at: 409 409
Size: 4448
PsiI TTA'TAA
Cuts at: 134 134
Size: 4448
PstI C_TGCA'G
Cuts at: 814 1495 2180 814
Size: 681 685 3082
Fragments arranged by size:
3082 685 681
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1873 2233 1873
Size: 360 4088
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 122 1462 122
Size: 1340 3108
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SanDI GG'GwC_CC
Cuts at: 1104 1104
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 810 1209 1491 2176 3169 3360 33
Size: 777 399 282 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 399 282 191
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1019 1603 2518 3010 3272 1019
Size: 584 915 492 262 2195
Fragments arranged by size:
2195 915 584 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StyI C'CwwG_G
Cuts at: 107 570 1582 1936 107
Size: 463 1012 354 2619
Fragments arranged by size:
2619 1012 463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2164 2623 2832 2031
Size: 133 459 209 3647
Fragments arranged by size:
3647 459 209 133
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1943 1943
Size: 4448
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
ZraI GAC'GTC
Cuts at: 407 407
Size: 4448
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI