Restriction map for pF1KB8830
 (Circular) (Six-base) MAPSORT of: pF1KB8830.seq  Check: 4739  from: 1  to: 4433



pF1KB8830  4433 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:   1471    1471 
   Size:       4433
 
Acc65I G'GTAC_C

Cuts at:   1024    1449    1024 
   Size:        425    4008
 
AclI AA'CG_TT

Cuts at:   4273    4273 
   Size:       4433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    431     928    1384    2258    2690    3437    3705     431 
   Size:        497     456     874     432     747     268    1159

  Fragments arranged by size: 

                1159     874     747     497     456     432     268
 
AflIII A'CryG_T

Cuts at:   1785    2889    3866    1785 
   Size:       1104     977    2352

  Fragments arranged by size: 

                2352    1104     977
 
AgeI A'CCGG_T

Cuts at:   2821    2821 
   Size:       4433
 
AlwNI CAG_nnn'CTG

Cuts at:    253     390    1738    3305     253 
   Size:        137    1348    1567    1381

  Fragments arranged by size: 

                1567    1381    1348     137
 
ApaLI G'TGCA_C

Cuts at:   3203    3203 
   Size:       4433
 
ApoI r'AATT_y

Cuts at:    470     570     806    1437    3786    4042     470 
   Size:        100     236     631    2349     256     861

  Fragments arranged by size: 

                2349     861     631     256     236     100
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4433
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4433
 
AvaI C'yCGr_G

Cuts at:   1453    1453 
   Size:       4433
 
BamHI G'GATC_C

Cuts at:   1458    1458 
   Size:       4433
 
BanI G'GyrC_C

Cuts at:   1024    1449    1863    2111    2146    1024 
   Size:        425     414     248      35    3311

  Fragments arranged by size: 

                3311     425     414     248      35
 
BanII G_rGCy'C

Cuts at:   1187    1447    2477    1187 
   Size:        260    1030    3143

  Fragments arranged by size: 

                3143    1030     260
 
BbeI G_GCGC'C

Cuts at:   1867    2115    1867 
   Size:        248    4185
 
BbsI GAAGACnn'nnnn_

Cuts at:   1061    1152    1061 
   Size:         91    4342
 
BciVI GTATCCnnnnn_n'

Cuts at:   2331    3092    4433    2331 
   Size:        761    1341    2331

  Fragments arranged by size: 

                2331    1341     761
 
BclI T'GATC_A

Cuts at:   1953    1953 
   Size:       4433
 
BglII A'GATC_T

Cuts at:   1948    3985    1948 
   Size:       2037    2396
 
BlpI GC'TnA_GC

Cuts at:   1545    1545 
   Size:       4433
 
Bme1580I G_kGCm'C

Cuts at:   2058    2151    3207    2058 
   Size:         93    1056    3284

  Fragments arranged by size: 

                3284    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    978    1190    1645    1726    2061    4205     978 
   Size:        212     455      81     335    2144    1206

  Fragments arranged by size: 

                2144    1206     455     335     212      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2800    2800 
   Size:       4433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1609    2488    2980    3278    1609 
   Size:        879     492     298    2764

  Fragments arranged by size: 

                2764     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4137    4137 
   Size:       4433
 
BsaAI yAC'GTr

Cuts at:   1786    1786 
   Size:       4433
 
BsaBI GATnn'nnATC

Cuts at:      6    1947    3984       6 
   Size:       1941    2037     455

  Fragments arranged by size: 

                2037    1941     455
 
BsaHI Gr'CG_yC

Cuts at:   1864    2112    2814    4310    1864 
   Size:        248     702    1496    1987

  Fragments arranged by size: 

                1987    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1622    2143    2821    3095    3242    3653    1622 
   Size:        521     678     274     147     411    2402

  Fragments arranged by size: 

                2402     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    159     189     923     953     159 
   Size:         30     734      30    3639

  Fragments arranged by size: 

                3639     734      30      30
 
BseYI C'CCAG_C

Cuts at:   1858    2616    3193    3689    1858 
   Size:        758     577     496    2602

  Fragments arranged by size: 

                2602     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1975    2021    3229     104 
   Size:       1871      46    1208    1308

  Fragments arranged by size: 

                1871    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1447    2225    2415    3207    1447 
   Size:        778     190     792    2673

  Fragments arranged by size: 

                2673     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1187    1447    2058    2151    2225    2415    2477    3207    1187 
   Size:        260     611      93      74     190      62     730    2413

  Fragments arranged by size: 

                2413     730     611     260     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3653    3653 
   Size:       4433
 
BspHI T'CATG_A

Cuts at:   3609    3609 
   Size:       4433
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1483    1697    1999    2380    1483 
   Size:        214     302     381    3536

  Fragments arranged by size: 

                3536     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2725    2779    4263    4428      50 
   Size:       2675      54    1484     165      55

  Fragments arranged by size: 

                2675    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1429    2345    1429 
   Size:        916    3517
 
BsrFI r'CCGG_y

Cuts at:   2431    2612    2821    2431 
   Size:        181     209    4043

  Fragments arranged by size: 

                4043     209     181
 
BsrGI T'GTAC_A

Cuts at:   1306    1306 
   Size:       4433
 
BssSI C'ACGA_G

Cuts at:   2704    3062    2704 
   Size:        358    4075
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1810    1810 
   Size:       4433
 
BstBI TT'CG_AA

Cuts at:   2794    2794 
   Size:       4433
 
BstXI CCAn_nnnn'nTGG

Cuts at:    333     968     333 
   Size:        635    3798
 
BstYI r'GATC_y

Cuts at:      1    1458    1925    1948    2283    2529    3541    3627    3639 
   Size:       1457     467      23     335     246    1012      86      12

Cuts at:   3639    3985       1 
   Size:        346     449

  Fragments arranged by size: 

                1457    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     401     107 
   Size:        294    4139
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2383    2564    2727    2383 
   Size:        181     163    4089

  Fragments arranged by size: 

                4089     181     163
 
BtsI GCAGTG_nn'

Cuts at:    415     557    1676     415 
   Size:        142    1119    3172

  Fragments arranged by size: 

                3172    1119     142
 
ClaI AT'CG_AT

Cuts at:    360    1325     360 
   Size:        965    3468
 
DraI TTT'AAA

Cuts at:   1432    1432 
   Size:       4433
 
DrdI GACnn_nn'nnGTC

Cuts at:   1088    1156    2139    2997    1088 
   Size:         68     983     858    2524

  Fragments arranged by size: 

                2524     983     858      68
 
EaeI y'GGCC_r

Cuts at:   2018    2192    2583    2610    3955    2018 
   Size:        174     391      27    1345    2496

  Fragments arranged by size: 

                2496    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2018    2018 
   Size:       4433
 
EarI CTCTTCn'nnn_

Cuts at:    450    1103    2456    2666     450 
   Size:        653    1353     210    2217

  Fragments arranged by size: 

                2217    1353     653     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2641    2951    3097    4243    2641 
   Size:        310     146    1146    2831

  Fragments arranged by size: 

                2831    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1445    1445 
   Size:       4433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    431     928    1384    2258    2690    3437    3705     431 
   Size:        497     456     874     432     747     268    1159

  Fragments arranged by size: 

                1159     874     747     497     456     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    730    1203    1572     730 
   Size:        473     369    3591

  Fragments arranged by size: 

                3591     473     369
 
EcoRI G'AATT_C

Cuts at:   1437    1437 
   Size:       4433
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2452    2452 
   Size:       4433
 
FspI TGC'GCA

Cuts at:   2214    2214 
   Size:       4433
 
HaeII r_GCGC'y

Cuts at:   1214    1867    2115    3137    4116    1214 
   Size:        653     248    1022     979    1531

  Fragments arranged by size: 

                1531    1022     979     653     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    612     644    2275    2305    2307    2337     612 
   Size:         32    1631      30       2      30    2708

  Fragments arranged by size: 

                2708    1631      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1472    1472 
   Size:       4433
 
KasI G'GCGC_C

Cuts at:   1863    2111    1863 
   Size:        248    4185
 
KpnI G_GTAC'C

Cuts at:   1028    1453    1028 
   Size:        425    4008
 
MluI A'CGCG_T

Cuts at:   3866    3866 
   Size:       4433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    145     314     860    3104    3288     145 
   Size:        169     546    2244     184    1290

  Fragments arranged by size: 

                2244    1290     546     184     169
 
MscI TGG'CCA

Cuts at:   2194    2194 
   Size:       4433
 
MslI CAynn'nnrTG

Cuts at:    350     479     680    2422    2549     350 
   Size:        129     201    1742     127    2234

  Fragments arranged by size: 

                2234    1742     201     129     127
 
MspA1I CmG'CkG

Cuts at:    317    1544    1858    2218    3231    3476     317 
   Size:       1227     314     360    1013     245    1274

  Fragments arranged by size: 

                1274    1227    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2614    2614 
   Size:       4433
 
NarI GG'CG_CC

Cuts at:   1864    2112    1864 
   Size:        248    4185
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4433
 
NgoMIV G'CCGG_C

Cuts at:   2612    2612 
   Size:       4433
 
NspI r_CATG'y

Cuts at:   1486    1986    2893    1486 
   Size:        500     907    3026

  Fragments arranged by size: 

                3026     907     500
 
PciI A'CATG_T

Cuts at:   2889    2889 
   Size:       4433
 
PmeI GTTT'AAAC

Cuts at:   1432    1432 
   Size:       4433
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3572    3604    3572 
   Size:         32    4401
 
PpuMI rG'GwC_Cy

Cuts at:    730    1203     730 
   Size:        473    3960
 
PsiI TTA'TAA

Cuts at:   1199    1379    1199 
   Size:        180    4253
 
PstI C_TGCA'G

Cuts at:    878    1277    1480    2165     878 
   Size:        399     203     685    3146

  Fragments arranged by size: 

                3146     685     399     203
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4433
 
PvuII CAG'CTG

Cuts at:    317    1858    2218     317 
   Size:       1541     360    2532

  Fragments arranged by size: 

                2532    1541     360
 
RsrII CG'GwC_CG

Cuts at:   2628    2628 
   Size:       4433
 
SacI G_AGCT'C

Cuts at:   1447    1447 
   Size:       4433
 
SalI G'TCGA_C

Cuts at:   1470    1470 
   Size:       4433
 
SapI GCTCTTCn'nnn_

Cuts at:   2456    2666    2456 
   Size:        210    4223
 
SbfI CC_TGCA'GG

Cuts at:   1480    1480 
   Size:       4433
 
ScaI AGT'ACT

Cuts at:   1397    3950    1397 
   Size:       2553    1880
 
SexAI A'CCwGG_T

Cuts at:    755     755 
   Size:       4433
 
SfcI C'TryA_G

Cuts at:     33     874    1086    1273    1476    2161    3154    3345      33 
   Size:        841     212     187     203     685     993     191    1121

  Fragments arranged by size: 

                1121     993     841     685     212     203     191     187
            
 
SfoI GGC'GCC

Cuts at:   1865    2113    1865 
   Size:        248    4185
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4433
 
SmaI CCC'GGG

Cuts at:   1455    1455 
   Size:       4433
 
SmlI C'TyrA_G

Cuts at:   1588    2503    2995    3257    1588 
   Size:        915     492     262    2764

  Fragments arranged by size: 

                2764     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     160      67 
   Size:         93    4340
 
SphI G_CATG'C

Cuts at:   1486    1986    1486 
   Size:        500    3933
 
SspI AAT'ATT

Cuts at:    129     129 
   Size:       4433
 
StyI C'CwwG_G

Cuts at:    107    1567    1921     107 
   Size:       1460     354    2619

  Fragments arranged by size: 

                2619    1460     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2016    2149    2608    2817    2016 
   Size:        133     459     209    3632

  Fragments arranged by size: 

                3632     459     209     133
 
TatI w'GTAC_w

Cuts at:   1306    1395    3948    1306 
   Size:         89    2553    1791

  Fragments arranged by size: 

                2553    1791      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    159     191    3950    3982     159 
   Size:         32    3759      32     610

  Fragments arranged by size: 

                3759     610      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2230    2230 
   Size:       4433
 
XbaI T'CTAG_A

Cuts at:   1464    1464 
   Size:       4433
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    855    1928     855 
   Size:       1073    3360
 
XmaI C'CCGG_G

Cuts at:   1453    1453 
   Size:       4433
 
XmnI GAAnn'nnTTC

Cuts at:   1232    1232 
   Size:       4433
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI
     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BseRI     BsgI    BsiWI     BsmI
    BsmBI   BssHII   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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