Restriction map for pF1KB8820
 (Circular) (Six-base) MAPSORT of: pF1KB8820.seq  Check: 5617  from: 1  to: 4265



pF1KB8820  4265 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:   1303    1303 
   Size:       4265
 
Acc65I G'GTAC_C

Cuts at:   1281    1281 
   Size:       4265
 
AclI AA'CG_TT

Cuts at:   4105    4105 
   Size:       4265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1096    2090    2522    3269    3537    1096 
   Size:        994     432     747     268    1824

  Fragments arranged by size: 

                1824     994     747     432     268
 
AflIII A'CryG_T

Cuts at:   1617    2721    3698    1617 
   Size:       1104     977    2184

  Fragments arranged by size: 

                2184    1104     977
 
AgeI A'CCGG_T

Cuts at:   2653    2653 
   Size:       4265
 
AlwNI CAG_nnn'CTG

Cuts at:   1074    1570    3137    1074 
   Size:        496    1567    2202

  Fragments arranged by size: 

                2202    1567     496
 
ApaLI G'TGCA_C

Cuts at:   3035    3035 
   Size:       4265
 
ApoI r'AATT_y

Cuts at:   1269    3618    3874    1269 
   Size:       2349     256    1660

  Fragments arranged by size: 

                2349    1660     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4265
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4265
 
AvaI C'yCGr_G

Cuts at:    578    1285     578 
   Size:        707    3558
 
AvrII C'CTAG_G

Cuts at:    756     756 
   Size:       4265
 
BamHI G'GATC_C

Cuts at:   1290    1290 
   Size:       4265
 
BanI G'GyrC_C

Cuts at:   1106    1113    1281    1695    1943    1978    1106 
   Size:          7     168     414     248      35    3393

  Fragments arranged by size: 

                3393     414     248     168      35       7
 
BanII G_rGCy'C

Cuts at:   1279    2309    1279 
   Size:       1030    3235
 
BbeI G_GCGC'C

Cuts at:   1699    1947    1699 
   Size:        248    4017
 
BbsI GAAGACnn'nnnn_

Cuts at:    240     252     432     240 
   Size:         12     180    4073

  Fragments arranged by size: 

                4073     180      12
 
BbvCI CC'TCA_GC

Cuts at:    289     571     826     289 
   Size:        282     255    3728

  Fragments arranged by size: 

                3728     282     255
 
BciVI GTATCCnnnnn_n'

Cuts at:   2163    2924    4265    2163 
   Size:        761    1341    2163

  Fragments arranged by size: 

                2163    1341     761
 
BclI T'GATC_A

Cuts at:   1785    1785 
   Size:       4265
 
BglI GCCn_nnn'nGGC

Cuts at:    339     339 
   Size:       4265
 
BglII A'GATC_T

Cuts at:    353    1780    3817     353 
   Size:       1427    2037     801

  Fragments arranged by size: 

                2037    1427     801
 
BlpI GC'TnA_GC

Cuts at:    554    1377     554 
   Size:        823    3442
 
Bme1580I G_kGCm'C

Cuts at:   1890    1983    3039    1890 
   Size:         93    1056    3116

  Fragments arranged by size: 

                3116    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    250    1477    1558    1893    4037     250 
   Size:       1227      81     335    2144     478

  Fragments arranged by size: 

                2144    1227     478     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2632    2632 
   Size:       4265
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    559     973    1035     559 
   Size:        414      62    3789

  Fragments arranged by size: 

                3789     414      62
 
Bpu10I CC'TnA_GC

Cuts at:    289     571     826     289 
   Size:        282     255    3728

  Fragments arranged by size: 

                3728     282     255
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1441    2320    2812    3110    1441 
   Size:        879     492     298    2596

  Fragments arranged by size: 

                2596     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3969    3969 
   Size:       4265
 
BsaAI yAC'GTr

Cuts at:    330    1618     330 
   Size:       1288    2977
 
BsaBI GATnn'nnATC

Cuts at:      6    1779    3816       6 
   Size:       1773    2037     455

  Fragments arranged by size: 

                2037    1773     455
 
BsaHI Gr'CG_yC

Cuts at:   1696    1944    2646    4142    1696 
   Size:        248     702    1496    1819

  Fragments arranged by size: 

                1819    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1454    1975    2653    2927    3074    3485    1454 
   Size:        521     678     274     147     411    2234

  Fragments arranged by size: 

                2234     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    108     138     108 
   Size:         30    4235
 
BseYI C'CCAG_C

Cuts at:   1690    2448    3025    3521    1690 
   Size:        758     577     496    2434

  Fragments arranged by size: 

                2434     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1807    1853    3061     104 
   Size:       1703      46    1208    1308

  Fragments arranged by size: 

                1703    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    472    1279    2057    2247    3039     472 
   Size:        807     778     190     792    1698

  Fragments arranged by size: 

                1698     807     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    636     636 
   Size:       4265
 
Bsp1286I G_dGCh'C

Cuts at:    472    1279    1890    1983    2057    2247    2309    3039     472 
   Size:        807     611      93      74     190      62     730    1698

  Fragments arranged by size: 

                1698     807     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3485    3485 
   Size:       4265
 
BspHI T'CATG_A

Cuts at:   3441    3441 
   Size:       4265
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    947    1315    1529    1831    2212     947 
   Size:        368     214     302     381    3000

  Fragments arranged by size: 

                3000     381     368     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2557    2611    4095    4260      50 
   Size:       2507      54    1484     165      55

  Fragments arranged by size: 

                2507    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2177    2177 
   Size:       4265
 
BsrFI r'CCGG_y

Cuts at:   2263    2444    2653    2263 
   Size:        181     209    3875

  Fragments arranged by size: 

                3875     209     181
 
BsrGI T'GTAC_A

Cuts at:    654     654 
   Size:       4265
 
BssSI C'ACGA_G

Cuts at:   2536    2894    2536 
   Size:        358    3907
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1642    1642 
   Size:       4265
 
BstBI TT'CG_AA

Cuts at:   2626    2626 
   Size:       4265
 
BstYI r'GATC_y

Cuts at:      1     353    1290    1757    1780    2115    2361    3373    3459 
   Size:        352     937     467      23     335     246    1012      86

Cuts at:   3459    3471    3817       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     937     467     449     352     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     422     107 
   Size:        315    3950
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    990    2215    2396    2559     990 
   Size:       1225     181     163    2696

  Fragments arranged by size: 

                2696    1225     181     163
 
BtsI GCAGTG_nn'

Cuts at:    116     233     400    1017    1121    1508     116 
   Size:        117     167     617     104     387    2873

  Fragments arranged by size: 

                2873     617     387     167     117     104
 
DraI TTT'AAA

Cuts at:    854    1264     854 
   Size:        410    3855
 
DrdI GACnn_nn'nnGTC

Cuts at:   1971    2829    1971 
   Size:        858    3407
 
EaeI y'GGCC_r

Cuts at:    110     371     626    1850    2024    2415    2442    3787     110 
   Size:        261     255    1224     174     391      27    1345     588

  Fragments arranged by size: 

                1345    1224     588     391     261     255     174      27
            
 
EagI C'GGCC_G

Cuts at:   1850    1850 
   Size:       4265
 
EarI CTCTTCn'nnn_

Cuts at:    736    2288    2498     736 
   Size:       1552     210    2503

  Fragments arranged by size: 

                2503    1552     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    357    1054    2473    2783    2929    4075     357 
   Size:        697    1419     310     146    1146     547

  Fragments arranged by size: 

                1419    1146     697     547     310     146
 
EcoICRI GAG'CTC

Cuts at:   1277    1277 
   Size:       4265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    559     973    1035    1096    2090    2522    3269    3537     559 
   Size:        414      62      61     994     432     747     268    1287

  Fragments arranged by size: 

                1287     994     747     432     414     268      62      61
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    564     564 
   Size:       4265
 
EcoO109I rG'GnC_Cy

Cuts at:    575    1404     575 
   Size:        829    3436
 
EcoRI G'AATT_C

Cuts at:   1269    1269 
   Size:       4265
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2284    2284 
   Size:       4265
 
FspI TGC'GCA

Cuts at:   2046    2046 
   Size:       4265
 
HaeII r_GCGC'y

Cuts at:   1699    1947    2969    3948    1699 
   Size:        248    1022     979    2016

  Fragments arranged by size: 

                2016    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1198    1230    2107    2137    2139    2169    1198 
   Size:         32     877      30       2      30    3294

  Fragments arranged by size: 

                3294     877      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1304    1304 
   Size:       4265
 
KasI G'GCGC_C

Cuts at:   1695    1943    1695 
   Size:        248    4017
 
KpnI G_GTAC'C

Cuts at:   1285    1285 
   Size:       4265
 
MluI A'CGCG_T

Cuts at:   3698    3698 
   Size:       4265
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    404     914    2936    3120     404 
   Size:        510    2022     184    1549

  Fragments arranged by size: 

                2022    1549     510     184
 
MscI TGG'CCA

Cuts at:    373    2026     373 
   Size:       1653    2612
 
MslI CAynn'nnrTG

Cuts at:    421    2254    2381     421 
   Size:       1833     127    2305

  Fragments arranged by size: 

                2305    1833     127
 
MspA1I CmG'CkG

Cuts at:    293    1145    1376    1690    2050    3063    3308     293 
   Size:        852     231     314     360    1013     245    1250

  Fragments arranged by size: 

                1250    1013     852     360     314     245     231
 
NaeI GCC'GGC

Cuts at:   2446    2446 
   Size:       4265
 
NarI GG'CG_CC

Cuts at:   1696    1944    1696 
   Size:        248    4017
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4265
 
NgoMIV G'CCGG_C

Cuts at:   2444    2444 
   Size:       4265
 
NspI r_CATG'y

Cuts at:   1318    1818    2725    1318 
   Size:        500     907    2858

  Fragments arranged by size: 

                2858     907     500
 
PciI A'CATG_T

Cuts at:   2721    2721 
   Size:       4265
 
PflMI CCAn_nnn'nTGG

Cuts at:    611     611 
   Size:       4265
 
PmeI GTTT'AAAC

Cuts at:   1264    1264 
   Size:       4265
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3404    3436    3404 
   Size:         32    4233
 
PpuMI rG'GwC_Cy

Cuts at:    575     575 
   Size:       4265
 
PstI C_TGCA'G

Cuts at:    217     867    1312    1997     217 
   Size:        650     445     685    2485

  Fragments arranged by size: 

                2485     685     650     445
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4265
 
PvuII CAG'CTG

Cuts at:   1145    1690    2050    1145 
   Size:        545     360    3360

  Fragments arranged by size: 

                3360     545     360
 
RsrII CG'GwC_CG

Cuts at:   2460    2460 
   Size:       4265
 
SacI G_AGCT'C

Cuts at:   1279    1279 
   Size:       4265
 
SalI G'TCGA_C

Cuts at:   1302    1302 
   Size:       4265
 
SapI GCTCTTCn'nnn_

Cuts at:    736    2288    2498     736 
   Size:       1552     210    2503

  Fragments arranged by size: 

                2503    1552     210
 
SbfI CC_TGCA'GG

Cuts at:   1312    1312 
   Size:       4265
 
ScaI AGT'ACT

Cuts at:   3782    3782 
   Size:       4265
 
SfcI C'TryA_G

Cuts at:     33     213     264     863    1308    1993    2986    3177      33 
   Size:        180      51     599     445     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     599     445     191     180      51
            
 
SfoI GGC'GCC

Cuts at:   1697    1945    1697 
   Size:        248    4017
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4265
 
SmaI CCC'GGG

Cuts at:    580    1287     580 
   Size:        707    3558
 
SmlI C'TyrA_G

Cuts at:   1420    2335    2827    3089    1420 
   Size:        915     492     262    2596

  Fragments arranged by size: 

                2596     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4265
 
SphI G_CATG'C

Cuts at:   1318    1818    1318 
   Size:        500    3765
 
StuI AGG'CCT

Cuts at:   1132    1132 
   Size:       4265
 
StyI C'CwwG_G

Cuts at:    107     674     756     869    1200    1399    1753     107 
   Size:        567      82     113     331     199     354    2619

  Fragments arranged by size: 

                2619     567     354     331     199     113      82
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1848    1981    2440    2649    1848 
   Size:        133     459     209    3464

  Fragments arranged by size: 

                3464     459     209     133
 
TatI w'GTAC_w

Cuts at:    654    3780     654 
   Size:       3126    1139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    108     140    3782    3814     108 
   Size:         32    3642      32     559

  Fragments arranged by size: 

                3642     559      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2062    2062 
   Size:       4265
 
XbaI T'CTAG_A

Cuts at:    975    1296     975 
   Size:        321    3944
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    642    1760     642 
   Size:       1118    3147
 
XmaI C'CCGG_G

Cuts at:    578    1285     578 
   Size:        707    3558
 
XmnI GAAnn'nnTTC

Cuts at:    730    1081     730 
   Size:        351    3914
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI     BcgI    BfrBI    BmgBI     BmtI    BseRI
     BsgI    BsiWI     BsmI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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