Restriction map for pF1KB8817
 (Linear) (Six-base) MAPSORT of: KIBB8817.seq  Check: 2476  from: 1  to: 1143



KIBB8817.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     309     458    1143 
   Size:        309     149     685

  Fragments arranged by size: 

                 685     309     149
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1143 
   Size:        919     224
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     393     891    1143 
   Size:         22     371     498     252

  Fragments arranged by size: 

                 498     371     252      22
 
ApoI r'AATT_y

Cuts at:      0     229    1143 
   Size:        229     914
 
BanI G'GyrC_C

Cuts at:      0     375    1143 
   Size:        375     768
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     417    1143 
   Size:        417     726
 
BclI T'GATC_A

Cuts at:      0     224    1143 
   Size:        224     919
 
BglI GCCn_nnn'nGGC

Cuts at:      0     274     535    1143 
   Size:        274     261     608

  Fragments arranged by size: 

                 608     274     261
 
BglII A'GATC_T

Cuts at:      0     285     476    1143 
   Size:        285     191     667

  Fragments arranged by size: 

                 667     285     191
 
Bme1580I G_kGCm'C

Cuts at:      0     380     531    1143 
   Size:        380     151     612

  Fragments arranged by size: 

                 612     380     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1108    1143 
   Size:        776     332      35

  Fragments arranged by size: 

                 776     332      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     107    1143 
   Size:        107    1036
 
BsaBI GATnn'nnATC

Cuts at:      0     577    1143 
   Size:        577     566
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643    1143 
   Size:        643     500
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35    1143 
   Size:         35    1108
 
BsiHKAI G_wGCw'C

Cuts at:      0     949    1143 
   Size:        949     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     380     531     949    1143 
   Size:        380     151     418     194

  Fragments arranged by size: 

                 418     380     194     151
 
BsrDI GCAATG_nn'

Cuts at:      0     263    1143 
   Size:        263     880
 
BsrFI r'CCGG_y

Cuts at:      0     440    1143 
   Size:        440     703
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     807    1143 
   Size:        807     336
 
BstYI r'GATC_y

Cuts at:      0     285     476    1143 
   Size:        285     191     667

  Fragments arranged by size: 

                 667     285     191
 
DraIII CAC_nnn'GTG

Cuts at:      0     305     375    1143 
   Size:        305      70     768

  Fragments arranged by size: 

                 768     305      70
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     886    1143 
   Size:        886     257
 
EaeI y'GGCC_r

Cuts at:      0     548    1143 
   Size:        548     595
 
EarI CTCTTCn'nnn_

Cuts at:      0     628    1143 
   Size:        628     515
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776     919    1108    1143 
   Size:        776     143     189      35

  Fragments arranged by size: 

                 776     189     143      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     355     390     888    1143 
   Size:        355      35     498     255

  Fragments arranged by size: 

                 498     355     255      35
 
FspI TGC'GCA

Cuts at:      0     244    1143 
   Size:        244     899
 
FspAI rTGC'GCAy

Cuts at:      0     244    1143 
   Size:        244     899
 
HincII GTy'rAC

Cuts at:      0     873    1143 
   Size:        873     270
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1143 
   Size:       1016     127
 
MscI TGG'CCA

Cuts at:      0     550    1143 
   Size:        550     593
 
MslI CAynn'nnrTG

Cuts at:      0     802    1143 
   Size:        802     341
 
MspA1I CmG'CkG

Cuts at:      0     839    1022    1032    1143 
   Size:        839     183      10     111

  Fragments arranged by size: 

                 839     183     111      10
 
NspI r_CATG'y

Cuts at:      0     243    1143 
   Size:        243     900
 
PasI CC'CwG_GG

Cuts at:      0     387    1143 
   Size:        387     756
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39     393     891    1143 
   Size:         39     354     498     252

  Fragments arranged by size: 

                 498     354     252      39
 
PfoI T'CCnGG_A

Cuts at:      0     557     584     753     890    1143 
   Size:        557      27     169     137     253

  Fragments arranged by size: 

                 557     253     169     137      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     355     888    1143 
   Size:        355     533     255

  Fragments arranged by size: 

                 533     355     255
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     137     169     976    1008    1143 
   Size:        137      32     807      32     135

  Fragments arranged by size: 

                 807     137     135      32      32
 
PvuII CAG'CTG

Cuts at:      0     839    1143 
   Size:        839     304
 
SapI GCTCTTCn'nnn_

Cuts at:      0     628    1143 
   Size:        628     515
 
SexAI A'CCwGG_T

Cuts at:      0     371    1143 
   Size:        371     772
 
SfcI C'TryA_G

Cuts at:      0     654     993    1143 
   Size:        654     339     150

  Fragments arranged by size: 

                 654     339     150
 
SphI G_CATG'C

Cuts at:      0     243    1143 
   Size:        243     900
 
StyI C'CwwG_G

Cuts at:      0     206    1143 
   Size:        206     937
 
TatI w'GTAC_w

Cuts at:      0     399     607     905    1143 
   Size:        399     208     298     238

  Fragments arranged by size: 

                 399     298     238     208
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     276     751     804    1143 
   Size:        276     475      53     339

  Fragments arranged by size: 

                 475     339     276      53
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     BanI    BciVI     BclI     BglI
    BglII Bme1580I     BpmI   Bpu10I    BsaBI    BseRI     BsgI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI    BstXI    BstYI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I     FspI    FspAI   HincII     MmeI     MscI
     MslI   MspA1I     NspI     PasI    PflMI     PfoI    PpuMI     PsrI
    PvuII     SapI    SexAI     SfcI     SphI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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