Restriction map for pF1KB8816
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       BstUI       BstXI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        Ksp632I     
KspI        MamI        MluI        MroI        MslI        
MvnI        NaeI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        224  GGTACC               G^GTACC
AccI          604  GTATAC               GT^[AC][GT]AC
AcyI           55  GGCGCC               G[GA]^CG[CT]C
AlwNI         784  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          845  GGGCCC               GGGCC^C
Asp718I       224  GGTACC               G^GTACC
BalI         1097  TGGCCA               TGG^CCA
BamHI         525  GGATCC               G^GATCC
BbeI           55  GGCGCC               GGCGC^C
BbvCI         115  GCTGAGG              CCTCAGC(-5/-2)
BsaHI          55  GGCGCC               G[GA]^CG[CT]C
BsaI          490  GGTCTC               GGTCTC(1/5)
BsgI          658  GTGCAG               GTGCAG(16/14)
BsmAI         491  GTCTC                GTCTC(1/5)
BsmI          576  GAATGC               GAATGC(1/-1)
BsrDI         200  GCAATG               GCAATG(2/0)
Bst1107I      604  GTATAC               GTA^TAC
BstEII         27  GGTGACC              G^GT[ACGT]ACC
BstPI          27  GGTGACC              G^GT[ACGT]ACC
BstZ17I       604  GTATAC               GTA^TAC
DraI         1008  TTTAAA               TTT^AAA
DraII         844  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EaeI         1097  TGGCCA               [CT]^GGCC[GA]
EcoO109I      844  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I        27  GGTGACC              G^GT[ACGT]ACC
EcoT14I       232  CCTTGG               C^C[AT][AT]GG
EcoT22I       455  ATGCAT               ATGCA^T
FauI          760  GCGGG                CCCGC(4/6)
HaeII          55  GGCGCC               [GA]GCGC^[CT]
HapII         297  CCGG                 C^CGG
Hin1I          55  GGCGCC               G[GA]^CG[CT]C
HpaII         297  CCGG                 C^CGG
KasI           55  GGCGCC               G^GCGCC
KpnI          224  GGTACC               GGTAC^C
MaeII         303  ACGT                 A^CGT
MfeI          497  CAATTG               C^AATTG
MluNI        1097  TGGCCA               TGG^CCA
MscI         1097  TGGCCA               TGG^CCA
MspA1I        758  CAGCGG               C[AC]G^C[GT]G
MspI          297  CCGG                 C^CGG
MunI          497  CAATTG               C^AATTG
NarI           55  GGCGCC               GG^CGCC
NdeI         1114  CATATG               CA^TATG
NsiI          455  ATGCAT               ATGCA^T
NspI          716  ACATGC               [GA]CATG^[CT]
SfaNI         142  GATGC                GCATC(5/9)
SfoI           55  GGCGCC               GGC^GCC
SmaI          296  CCCGGG               CCC^GGG
StuI           36  AGGCCT               AGG^CCT
StyI          232  CCTTGG               C^C[AT][AT]GG
TspRI         863  CACTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         296  CCCGGG               C^CCGGG
XmaI          296  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI           32  CCGC                 CCGC(-3/-1)
              760  GCGG                
AfaI          225  GTAC                 GT^AC
              853  GTAC                
Asp700I       898  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              975  GAATCAATTC          
AvaI          296  CCCGGG               C^[CT]CG[GA]G
              521  CTCGGG              
BanI           55  GGCGCC               G^G[CT][GA]CC
              224  GGTACC              
BcnI          296  CCCGG                CC^[GC]GG
              297  CCGGG               
BsoBI         296  CCCGGG               C^[CT]CG[GA]G
              521  CTCGGG              
BsrI          176  ACTGG                ACTGG(1/-1)
              864  ACTGG               
BstF5I        132  GGATG                GGATG(2/0)
              138  GGATG               
BstYI         124  GGATCT               [GA]^GATC[CT]
              525  GGATCC              
CfoI           56  GCGC                 GCG^C
              591  GCGC                
FokI          132  GGATG                GGATG(9/13)
              138  GGATG               
HhaI           56  GCGC                 GCG^C
              591  GCGC                
HinP1I         56  GCGC                 G^CGC
              591  GCGC                
MflI          124  GGATCT               [GA]^GATC[CT]
              525  GGATCC              
NciI          296  CCCGG                CC^[GC]GG
              297  CCGGG               
RsaI          225  GTAC                 GT^AC
              853  GTAC                
SmlI          359  CTCAAG               C^T[CT][GA]AG
              567  CTTGAG              
Tsp45I         28  GTGAC                ^GT[GC]AC
             1055  GTCAC               
XhoII         124  GGATCT               [GA]^GATC[CT]
              525  GGATCC              
XmnI          898  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              975  GAATCAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BanII       
BbvI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      HinfI       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TfiI        
Tru9I       TseI        Tsp509I     
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