Restriction map for pF1KB8796
 (Circular) (Six-base) MAPSORT of: pF1KB8796.seq  Check: 7747  from: 1  to: 4112



pF1KB8796  4112 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    668    1150     668 
   Size:        482    3630
 
Acc65I G'GTAC_C

Cuts at:    653    1128     653 
   Size:        475    3637
 
AclI AA'CG_TT

Cuts at:   3952    3952 
   Size:       4112
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    142    1937    2369    3116    3384     142 
   Size:       1795     432     747     268     870

  Fragments arranged by size: 

                1795     870     747     432     268
 
AflIII A'CryG_T

Cuts at:   1005    1464    2568    3545    1005 
   Size:        459    1104     977    1572

  Fragments arranged by size: 

                1572    1104     977     459
 
AgeI A'CCGG_T

Cuts at:   2500    2500 
   Size:       4112
 
AlwNI CAG_nnn'CTG

Cuts at:    855    1417    2984     855 
   Size:        562    1567    1983

  Fragments arranged by size: 

                1983    1567     562
 
ApaLI G'TGCA_C

Cuts at:   2882    2882 
   Size:       4112
 
ApoI r'AATT_y

Cuts at:   1116    3465    3721    1116 
   Size:       2349     256    1507

  Fragments arranged by size: 

                2349    1507     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4112
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4112
 
AvaI C'yCGr_G

Cuts at:   1132    1132 
   Size:       4112
 
BamHI G'GATC_C

Cuts at:   1137    1137 
   Size:       4112
 
BanI G'GyrC_C

Cuts at:    653     764    1041    1075    1093    1128    1542    1790    1825 
   Size:        111     277      34      18      35     414     248      35

Cuts at:   1825     653 
   Size:       2940

  Fragments arranged by size: 

                2940     414     277     248     111      35      35      34
                  18
 
BanII G_rGCy'C

Cuts at:    508    1126    2156     508 
   Size:        618    1030    2464

  Fragments arranged by size: 

                2464    1030     618
 
BbeI G_GCGC'C

Cuts at:    768    1097    1546    1794     768 
   Size:        329     449     248    3086

  Fragments arranged by size: 

                3086     449     329     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    963     963 
   Size:       4112
 
BbvCI CC'TCA_GC

Cuts at:    238     814     238 
   Size:        576    3536
 
BciVI GTATCCnnnnn_n'

Cuts at:   2010    2771    4112    2010 
   Size:        761    1341    2010

  Fragments arranged by size: 

                2010    1341     761
 
BclI T'GATC_A

Cuts at:   1632    1632 
   Size:       4112
 
BglI GCCn_nnn'nGGC

Cuts at:    248     248 
   Size:       4112
 
BglII A'GATC_T

Cuts at:   1627    3664    1627 
   Size:       2037    2075
 
BlpI GC'TnA_GC

Cuts at:   1224    1224 
   Size:       4112
 
Bme1580I G_kGCm'C

Cuts at:   1737    1830    2886    1737 
   Size:         93    1056    2963

  Fragments arranged by size: 

                2963    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    393    1324    1405    1740    3884     393 
   Size:        931      81     335    2144     621

  Fragments arranged by size: 

                2144     931     621     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    424    1113    2479     424 
   Size:        689    1366    2057

  Fragments arranged by size: 

                2057    1366     689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    313     313 
   Size:       4112
 
Bpu10I CC'TnA_GC

Cuts at:    238     814     238 
   Size:        576    3536
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1288    2167    2659    2957    1288 
   Size:        879     492     298    2443

  Fragments arranged by size: 

                2443     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3816    3816 
   Size:       4112
 
BsaAI yAC'GTr

Cuts at:    650     723    1465     650 
   Size:         73     742    3297

  Fragments arranged by size: 

                3297     742      73
 
BsaBI GATnn'nnATC

Cuts at:      6    1626    3663       6 
   Size:       1620    2037     455

  Fragments arranged by size: 

                2037    1620     455
 
BsaHI Gr'CG_yC

Cuts at:    765    1094    1543    1791    2493    3989     765 
   Size:        329     449     248     702    1496     888

  Fragments arranged by size: 

                1496     888     702     449     329     248
 
BsaWI w'CCGG_w

Cuts at:    875    1301    1822    2500    2774    2921    3332     875 
   Size:        426     521     678     274     147     411    1655

  Fragments arranged by size: 

                1655     678     521     426     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    646     676     995    1025     646 
   Size:         30     319      30    3733

  Fragments arranged by size: 

                3733     319      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    226     409     226 
   Size:        183    3929
 
BseYI C'CCAG_C

Cuts at:    389     396     818    1045    1050    1537    2295    2872    3368 
   Size:          7     422     227       5     487     758     577     496

Cuts at:   3368     389 
   Size:       1133

  Fragments arranged by size: 

                1133     758     577     496     487     422     227       7
                   5
 
BsiEI CG_ry'CG

Cuts at:    104    1654    1700    2908     104 
   Size:       1550      46    1208    1308

  Fragments arranged by size: 

                1550    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    136     965    1126    1904    2094    2886     136 
   Size:        829     161     778     190     792    1362

  Fragments arranged by size: 

                1362     829     792     778     190     161
 
Bsp1286I G_dGCh'C

Cuts at:    136     508     965    1126    1737    1830    1904    2094    2156 
   Size:        372     457     161     611      93      74     190      62

Cuts at:   2156    2886     136 
   Size:        730    1362

  Fragments arranged by size: 

                1362     730     611     457     372     190     161      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:    875    3332     875 
   Size:       2457    1655
 
BspHI T'CATG_A

Cuts at:    473    3288     473 
   Size:       2815    1297
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1162    1376    1678    2059    1162 
   Size:        214     302     381    3215

  Fragments arranged by size: 

                3215     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     749    2404    2458    3942    4107      50 
   Size:        699    1655      54    1484     165      55

  Fragments arranged by size: 

                1655    1484     699     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2024    2024 
   Size:       4112
 
BsrFI r'CCGG_y

Cuts at:   1031    2110    2291    2500    1031 
   Size:       1079     181     209    2643

  Fragments arranged by size: 

                2643    1079     209     181
 
BssSI C'ACGA_G

Cuts at:   2383    2741    2383 
   Size:        358    3754
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1489    1489 
   Size:       4112
 
BstBI TT'CG_AA

Cuts at:   2473    2473 
   Size:       4112
 
BstEII G'GTnAC_C

Cuts at:    552     552 
   Size:       4112
 
BstXI CCAn_nnnn'nTGG

Cuts at:    383     801    1072     383 
   Size:        418     271    3423

  Fragments arranged by size: 

                3423     418     271
 
BstYI r'GATC_y

Cuts at:      1     368     728    1137    1604    1627    1962    2208    3220 
   Size:        367     360     409     467      23     335     246    1012

Cuts at:   3220    3306    3318    3664       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     409     367     360     346     335
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    117     733     117 
   Size:        616    3496
 
BtgI C'CryG_G

Cuts at:    107     557     107 
   Size:        450    3662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2062    2243    2406    2062 
   Size:        181     163    3768

  Fragments arranged by size: 

                3768     181     163
 
BtsI GCAGTG_nn'

Cuts at:    942    1355     942 
   Size:        413    3699
 
DraI TTT'AAA

Cuts at:   1111    1111 
   Size:       4112
 
DraIII CAC_nnn'GTG

Cuts at:    615     615 
   Size:       4112
 
DrdI GACnn_nn'nnGTC

Cuts at:   1818    2676    1818 
   Size:        858    3254
 
EaeI y'GGCC_r

Cuts at:   1697    1871    2262    2289    3634    1697 
   Size:        174     391      27    1345    2175

  Fragments arranged by size: 

                2175    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1697    1697 
   Size:       4112
 
EarI CTCTTCn'nnn_

Cuts at:    688    2135    2345     688 
   Size:       1447     210    2455

  Fragments arranged by size: 

                2455    1447     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2320    2630    2776    3922    2320 
   Size:        310     146    1146    2510

  Fragments arranged by size: 

                2510    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1124    1124 
   Size:       4112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     313    1937    2369    3116    3384     142 
   Size:        171    1624     432     747     268     870

  Fragments arranged by size: 

                1624     870     747     432     268     171
 
EcoO109I rG'GnC_Cy

Cuts at:    270     737     823    1251     270 
   Size:        467      86     428    3131

  Fragments arranged by size: 

                3131     467     428      86
 
EcoRI G'AATT_C

Cuts at:   1116    1116 
   Size:       4112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    804    2131     804 
   Size:       1327    2785
 
FspI TGC'GCA

Cuts at:   1893    1893 
   Size:       4112
 
HaeII r_GCGC'y

Cuts at:    768    1097    1546    1794    2816    3795     768 
   Size:        329     449     248    1022     979    1085

  Fragments arranged by size: 

                1085    1022     979     449     329     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1954    1984    1986    2016    1954 
   Size:         30       2      30    4050

  Fragments arranged by size: 

                4050      30      30       2
 
HincII GTy'rAC

Cuts at:   1151    1151 
   Size:       4112
 
KasI G'GCGC_C

Cuts at:    764    1093    1542    1790     764 
   Size:        329     449     248    3086

  Fragments arranged by size: 

                3086     449     329     248
 
KpnI G_GTAC'C

Cuts at:    657    1132     657 
   Size:        475    3637
 
MluI A'CGCG_T

Cuts at:   3545    3545 
   Size:       4112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2783    2967    2783 
   Size:        184    3928
 
MscI TGG'CCA

Cuts at:   1873    1873 
   Size:       4112
 
MslI CAynn'nnrTG

Cuts at:    562     799    2101    2228     562 
   Size:        237    1302     127    2446

  Fragments arranged by size: 

                2446    1302     237     127
 
MspA1I CmG'CkG

Cuts at:    539     818    1223    1537    1897    2910    3155     539 
   Size:        279     405     314     360    1013     245    1496

  Fragments arranged by size: 

                1496    1013     405     360     314     279     245
 
NaeI GCC'GGC

Cuts at:   1033    2293    1033 
   Size:       1260    2852
 
NarI GG'CG_CC

Cuts at:    765    1094    1543    1791     765 
   Size:        329     449     248    3086

  Fragments arranged by size: 

                3086     449     329     248
 
NcoI C'CATG_G

Cuts at:    107     557     107 
   Size:        450    3662
 
NgoMIV G'CCGG_C

Cuts at:   1031    2291    1031 
   Size:       1260    2852
 
NspI r_CATG'y

Cuts at:   1009    1165    1665    2572    1009 
   Size:        156     500     907    2549

  Fragments arranged by size: 

                2549     907     500     156
 
PasI CC'CwG_GG

Cuts at:    699     699 
   Size:       4112
 
PciI A'CATG_T

Cuts at:   1005    2568    1005 
   Size:       1563    2549
 
PmeI GTTT'AAAC

Cuts at:   1111    1111 
   Size:       4112
 
PmlI CAC'GTG

Cuts at:    650     650 
   Size:       4112
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3251    3283    3251 
   Size:         32    4080
 
PpuMI rG'GwC_Cy

Cuts at:    270     737     823     270 
   Size:        467      86    3559

  Fragments arranged by size: 

                3559     467      86
 
PstI C_TGCA'G

Cuts at:    851    1040    1159    1844     851 
   Size:        189     119     685    3119

  Fragments arranged by size: 

                3119     685     189     119
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4112
 
PvuII CAG'CTG

Cuts at:    818    1537    1897     818 
   Size:        719     360    3033

  Fragments arranged by size: 

                3033     719     360
 
RsrII CG'GwC_CG

Cuts at:   2307    2307 
   Size:       4112
 
SacI G_AGCT'C

Cuts at:   1126    1126 
   Size:       4112
 
SalI G'TCGA_C

Cuts at:   1149    1149 
   Size:       4112
 
SanDI GG'GwC_CC

Cuts at:    823     823 
   Size:       4112
 
SapI GCTCTTCn'nnn_

Cuts at:   2135    2345    2135 
   Size:        210    3902
 
SbfI CC_TGCA'GG

Cuts at:   1159    1159 
   Size:       4112
 
ScaI AGT'ACT

Cuts at:   3629    3629 
   Size:       4112
 
SexAI A'CCwGG_T

Cuts at:    338     338 
   Size:       4112
 
SfcI C'TryA_G

Cuts at:     33     447     847    1036    1155    1840    2833    3024      33 
   Size:        414     400     189     119     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     414     400     191     189     119
            
 
SfoI GGC'GCC

Cuts at:    766    1095    1544    1792     766 
   Size:        329     449     248    3086

  Fragments arranged by size: 

                3086     449     329     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4112
 
SmaI CCC'GGG

Cuts at:   1134    1134 
   Size:       4112
 
SmlI C'TyrA_G

Cuts at:   1267    2182    2674    2936    1267 
   Size:        915     492     262    2443

  Fragments arranged by size: 

                2443     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4112
 
SphI G_CATG'C

Cuts at:   1165    1665    1165 
   Size:        500    3612
 
StuI AGG'CCT

Cuts at:    184     184 
   Size:       4112
 
StyI C'CwwG_G

Cuts at:    107     557    1246    1600     107 
   Size:        450     689     354    2619

  Fragments arranged by size: 

                2619     689     450     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    381    1059    1695    1828    2287    2496     381 
   Size:        678     636     133     459     209    1997

  Fragments arranged by size: 

                1997     678     636     459     209     133
 
TatI w'GTAC_w

Cuts at:   3627    3627 
   Size:       4112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3629    3661    3629 
   Size:         32    4080
 
Tth111I GACn'n_nGTC

Cuts at:   1909    1909 
   Size:       4112
 
XbaI T'CTAG_A

Cuts at:   1143    1143 
   Size:       4112
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1607    1607 
   Size:       4112
 
XmaI C'CCGG_G

Cuts at:   1132    1132 
   Size:       4112
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII  BstZ17I     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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