Restriction map for pF1KB8785
 (Linear) (Six-base) MAPSORT of: KIBB8785.seq  Check: 7628  from: 1  to: 879



KIBB8785.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     274     879 
   Size:        274     605
 
AclI AA'CG_TT

Cuts at:      0      11     879 
   Size:         11     868
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     879 
   Size:        523     356
 
AflIII A'CryG_T

Cuts at:      0     384     879 
   Size:        384     495
 
AvaI C'yCGr_G

Cuts at:      0     188     879 
   Size:        188     691
 
BamHI G'GATC_C

Cuts at:      0      27     879 
   Size:         27     852
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     740     879 
   Size:        740     139
 
BbvCI CC'TCA_GC

Cuts at:      0     743     830     879 
   Size:        743      87      49

  Fragments arranged by size: 

                 743      87      49
 
BclI T'GATC_A

Cuts at:      0     806     879 
   Size:        806      73
 
Bme1580I G_kGCm'C

Cuts at:      0     425     879 
   Size:        425     454
 
BmtI G_CTAG'C

Cuts at:      0     230     879 
   Size:        230     649
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     879 
   Size:        820      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     743     830     879 
   Size:        743      87      49

  Fragments arranged by size: 

                 743      87      49
 
BsaAI yAC'GTr

Cuts at:      0     385     879 
   Size:        385     494
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     309     383     386     389     679     760     879 
   Size:        309      74       3       3     290      81     119

  Fragments arranged by size: 

                 309     290     119      81      74       3       3
 
BseYI C'CCAG_C

Cuts at:      0     378     879 
   Size:        378     501
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     879 
   Size:        749     130
 
Bsp1286I G_dGCh'C

Cuts at:      0     425     879 
   Size:        425     454
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     274     879 
   Size:        274     605
 
BsrDI GCAATG_nn'

Cuts at:      0     404     879 
   Size:        404     475
 
BsrGI T'GTAC_A

Cuts at:      0     368     879 
   Size:        368     511
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      24     411     879 
   Size:         24     387     468

  Fragments arranged by size: 

                 468     387      24
 
BstYI r'GATC_y

Cuts at:      0      27     502     879 
   Size:         27     475     377

  Fragments arranged by size: 

                 475     377      27
 
EaeI y'GGCC_r

Cuts at:      0       2     864     879 
   Size:          2     862      15

  Fragments arranged by size: 

                 862      15       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     656     879 
   Size:        656     223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     820     879 
   Size:        523     297      59

  Fragments arranged by size: 

                 523     297      59
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     459     879 
   Size:        459     420
 
EcoO109I rG'GnC_Cy

Cuts at:      0     753     879 
   Size:        753     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      15      19      47      51     879 
   Size:         15       4      28       4     828

  Fragments arranged by size: 

                 828      28      15       4       4
 
HincII GTy'rAC

Cuts at:      0     436     560     879 
   Size:        436     124     319

  Fragments arranged by size: 

                 436     319     124
 
MscI TGG'CCA

Cuts at:      0       4     866     879 
   Size:          4     862      13

  Fragments arranged by size: 

                 862      13       4
 
MspA1I CmG'CkG

Cuts at:      0     304     798     879 
   Size:        304     494      81

  Fragments arranged by size: 

                 494     304      81
 
NheI G'CTAG_C

Cuts at:      0     226     879 
   Size:        226     653
 
PfoI T'CCnGG_A

Cuts at:      0     590     879 
   Size:        590     289
 
PmlI CAC'GTG

Cuts at:      0     385     879 
   Size:        385     494
 
PpuMI rG'GwC_Cy

Cuts at:      0     753     879 
   Size:        753     126
 
PvuII CAG'CTG

Cuts at:      0     798     879 
   Size:        798      81
 
SanDI GG'GwC_CC

Cuts at:      0     753     879 
   Size:        753     126
 
SfcI C'TryA_G

Cuts at:      0     197     416     879 
   Size:        197     219     463

  Fragments arranged by size: 

                 463     219     197
 
SmaI CCC'GGG

Cuts at:      0     190     879 
   Size:        190     689
 
SrfI GCCC'GGGC

Cuts at:      0     190     879 
   Size:        190     689
 
TatI w'GTAC_w

Cuts at:      0     368     879 
   Size:        368     511
 
XmaI C'CCGG_G

Cuts at:      0     188     879 
   Size:        188     691
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AvaI    BamHI     BbsI    BbvCI
     BclI Bme1580I     BmtI     BpmI   Bpu10I    BsaAI    BseRI    BseYI
     BsgI Bsp1286I    BspMI    BsrDI    BsrGI    BstXI    BstYI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MscI   MspA1I
     NheI     PfoI     PmlI    PpuMI    PvuII    SanDI     SfcI     SmaI
     SrfI     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BplI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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