Restriction map for pF1KB8782
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AlwI        AlwNI       
AocI        ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AvrII       BamHI       
BbrPI       BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BpmI        
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrI        BssSI       Bst1107I    BstBI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        Eam1105I    EarI        Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        Ksp632I     MaeI        MamI        
MflI        MroI        MseI        NdeI        NheI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TfiI        Tru9I       TspRI       Van91I      XbaI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        106  GGTACC               G^GTACC
AflIII        530  ACGCGT               A^C[GA][CT]GT
Aor51HI       713  AGCGCT               AGC^GCT
Asp718I       106  GGTACC               G^GTACC
AspI          151  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         129  GGACC                G^G[AT]CC
AviII         348  TGCGCA               TGC^GCA
BanII         429  GAGCCC               G[GA]GC[CT]^C
BbsI          223  GTCTTC               GAAGAC(2/6)
BpuAI         223  GTCTTC               GAAGAC(2/6)
BsmAI           8  GAGAC                GTCTC(1/5)
BsmFI         809  GGGAC                GGGAC(10/14)
Bsp1407I      257  TGTACA               T^GTACA
BsrGI         257  TGTACA               T^GTACA
BstEII        615  GGTCACC              G^GT[ACGT]ACC
BstPI         615  GGTCACC              G^GT[ACGT]ACC
Eco47III      713  AGCGCT               AGC^GCT
EcoO65I       615  GGTCACC              G^GT[ACGT]ACC
FspI          348  TGCGCA               TGC^GCA
HincII        157  GTCAAC               GT[CT]^[GA]AC
HindII        157  GTCAAC               GT[CT]^[GA]AC
KpnI          106  GGTACC               GGTAC^C
KspI          770  CCGCGG               CCGC^GG
MboII         224  TCTTC                GAAGA(8/7)
MfeI           50  CAATTG               C^AATTG
MluI          530  ACGCGT               A^CGCGT
MspA1I        770  CCGCGG               C[AC]G^C[GT]G
MunI           50  CAATTG               C^AATTG
NcoI          483  CCATGG               C^CATGG
NotI          274  GCGGCCGC             GC^GGCCGC
NspI          626  GCATGC               [GA]CATG^[CT]
PaeR7I        700  CTCGAG               C^TCGAG
PflFI         151  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          742  CTGCAG               CTGCA^G
SacII         770  CCGCGG               CCGC^GG
SexAI         389  ACCTGGT              A^CC[AT]GGT
SfcI          742  CTGCAG               C^T[GA][CT]AG
SgrAI         357  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          626  GCATGC               GCATG^C
SspBI         257  TGTACA               T^GTACA
StuI          696  AGGCCT               AGG^CCT
Tsp509I        51  AATT                 ^AATT
Tth111I       151  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          700  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

Alw44I        217  GTGCAC               G^TGCAC
              394  GTGCAC              
ApaLI         217  GTGCAC               G^TGCAC
              394  GTGCAC              
AvaI          538  CCCGAG               C^[CT]CG[GA]G
              700  CTCGAG              
BalI           83  TGGCCA               TGG^CCA
              113  TGGCCA              
BbeI          361  GGCGCC               GGCGC^C
              665  GGCGCC              
BlpI          426  GCTGAGC              GC^T[ACGT]AGC
              518  GCTCAGC             
Bpu1102I      426  GCTGAGC              GC^T[ACGT]AGC
              518  GCTCAGC             
BsgI          376  CTGCAC               GTGCAG(16/14)
              301  GTGCAG              
BsiEI         275  CGGCCG               CG[GA][CT]^CG
              471  CGGCCG              
BsiYI         185  CCATCCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              766  CCCTCCGCGGG         
BslI          185  CCATCCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              766  CCCTCCGCGGG         
BsoBI         538  CCCGAG               C^[CT]CG[GA]G
              700  CTCGAG              
BspMI         287  ACCTGC               ACCTGC(4/8)
              374  ACCTGC              
BstAPI        210  GCACATCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              294  GCAAGCCGTGC         
BstXI         116  CCATCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              477  CCAGGCCCATGG        
CelII         426  GCTGAGC              GC^T[ACGT]AGC
              518  GCTCAGC             
DraII         722  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              763  AGGCCCT             
EagI          275  CGGCCG               C^GGCCG
              471  CGGCCG              
EclXI         275  CGGCCG               C^GGCCG
              471  CGGCCG              
Eco52I        275  CGGCCG               C^GGCCG
              471  CGGCCG              
EcoO109I      722  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              763  AGGCCCT             
EcoT14I        71  CCAAGG               C^C[AT][AT]GG
              483  CCATGG              
HinfI         640  GACTC                G^A[ACGT]TC
              811  GACTC               
KasI          361  GGCGCC               G^GCGCC
              665  GGCGCC              
MaeIII        534  GTCAC                ^GT[ACGT]AC
              616  GTCAC               
MluNI          83  TGGCCA               TGG^CCA
              113  TGGCCA              
MscI           83  TGGCCA               TGG^CCA
              113  TGGCCA              
NaeI          358  GCCGGC               GCC^GGC
              652  GCCGGC              
NarI          361  GGCGCC               GG^CGCC
              665  GGCGCC              
NgoMIV        358  GCCGGC               G^CCGGC
              652  GCCGGC              
PleI          640  GACTC                GAGTC(4/5)
              811  GACTC               
SfaNI         317  GCATC                GCATC(5/9)
              554  GCATC               
SfoI          361  GGCGCC               GGC^GCC
              665  GGCGCC              
SmlI          406  CTCAAG               C^T[CT][GA]AG
              700  CTCGAG              
StyI           71  CCAAGG               C^C[AT][AT]GG
              483  CCATGG              
Tsp45I        534  GTCAC                ^GT[GC]AC
              616  GTCAC               
XcmI           71  CCAAGGTGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              116  CCATCAAGGTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       BanI        BbvI        BcgI        BcnI        
BmyI        BsaHI       BsaJI       BsiHKAI     Bsp1286I    
BsrFI       BssHII      BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraIII      DsaI        EaeI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeII       
MboI        MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TaqI        TseI        TthHB8I     
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