Restriction map for pF1KB8782
 (Circular) (Six-base) MAPSORT of: pF1KB8782.seq  Check: 7103  from: 1  to: 3932



pF1KB8782  3932 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    970     970 
   Size:       3932
 
Acc65I G'GTAC_C

Cuts at:    214     948     214 
   Size:        734    3198
 
AclI AA'CG_TT

Cuts at:   3772    3772 
   Size:       3932
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1757    2189    2936    3204    1757 
   Size:        432     747     268    2485

  Fragments arranged by size: 

                2485     747     432     268
 
AfeI AGC'GCT

Cuts at:    823     823 
   Size:       3932
 
AflIII A'CryG_T

Cuts at:    638    1284    2388    3365     638 
   Size:        646    1104     977    1205

  Fragments arranged by size: 

                1205    1104     977     646
 
AgeI A'CCGG_T

Cuts at:   2320    2320 
   Size:       3932
 
AlwNI CAG_nnn'CTG

Cuts at:   1237    2804    1237 
   Size:       1567    2365
 
ApaLI G'TGCA_C

Cuts at:    325     502    2702     325 
   Size:        177    2200    1555

  Fragments arranged by size: 

                2200    1555     177
 
ApoI r'AATT_y

Cuts at:    936    3285    3541     936 
   Size:       2349     256    1327

  Fragments arranged by size: 

                2349    1327     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3932
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3932
 
AvaI C'yCGr_G

Cuts at:    646     808     952     646 
   Size:        162     144    3626

  Fragments arranged by size: 

                3626     162     144
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    198     231     198 
   Size:         33    3899
 
BamHI G'GATC_C

Cuts at:    957     957 
   Size:       3932
 
BanI G'GyrC_C

Cuts at:    214     469     773     948    1362    1610    1645     214 
   Size:        255     304     175     414     248      35    2501

  Fragments arranged by size: 

                2501     414     304     255     248     175      35
 
BanII G_rGCy'C

Cuts at:    541     946    1976     541 
   Size:        405    1030    2497

  Fragments arranged by size: 

                2497    1030     405
 
BbeI G_GCGC'C

Cuts at:    473     777    1366    1614     473 
   Size:        304     589     248    2791

  Fragments arranged by size: 

                2791     589     304     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    324     324 
   Size:       3932
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    436     470     687     721     723     757     436 
   Size:         34     217      34       2      34    3611

  Fragments arranged by size: 

                3611     217      34      34      34       2
 
BciVI GTATCCnnnnn_n'

Cuts at:   1830    2591    3932    1830 
   Size:        761    1341    1830

  Fragments arranged by size: 

                1830    1341     761
 
BclI T'GATC_A

Cuts at:   1452    1452 
   Size:       3932
 
BglII A'GATC_T

Cuts at:   1447    3484    1447 
   Size:       2037    1895
 
BlpI GC'TnA_GC

Cuts at:    535     627    1044     535 
   Size:         92     417    3423

  Fragments arranged by size: 

                3423     417      92
 
Bme1580I G_kGCm'C

Cuts at:    329     506    1557    1650    2706     329 
   Size:        177    1051      93    1056    1555

  Fragments arranged by size: 

                1555    1056    1051     177      93
 
BmgBI CAC'GTC

Cuts at:    330     330 
   Size:       3932
 
BmrI ACTGGGnnnn_n'

Cuts at:   1144    1225    1560    3704    1144 
   Size:         81     335    2144    1372

  Fragments arranged by size: 

                2144    1372     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2299    2299 
   Size:       3932
 
Bpu10I CC'TnA_GC

Cuts at:    607     607 
   Size:       3932
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    499    1108    1987    2479    2777     499 
   Size:        609     879     492     298    1654

  Fragments arranged by size: 

                1654     879     609     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3636    3636 
   Size:       3932
 
BsaAI yAC'GTr

Cuts at:   1285    1285 
   Size:       3932
 
BsaBI GATnn'nnATC

Cuts at:      6    1446    3483       6 
   Size:       1440    2037     455

  Fragments arranged by size: 

                2037    1440     455
 
BsaHI Gr'CG_yC

Cuts at:    470     704     774    1363    1611    2313    3809     470 
   Size:        234      70     589     248     702    1496     593

  Fragments arranged by size: 

                1496     702     593     589     248     234      70
 
BsaWI w'CCGG_w

Cuts at:   1121    1642    2320    2594    2741    3152    1121 
   Size:        521     678     274     147     411    1901

  Fragments arranged by size: 

                1901     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    147    1357    2115    2692    3188     147 
   Size:       1210     758     577     496     891

  Fragments arranged by size: 

                1210     891     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    430     469     430 
   Size:         39    3893
 
BsiEI CG_ry'CG

Cuts at:    104     386     582    1474    1520    2728     104 
   Size:        282     196     892      46    1208    1308

  Fragments arranged by size: 

                1308    1208     892     282     196      46
 
BsiHKAI G_wGCw'C

Cuts at:    329     506     613     656     713     794     946    1724    1914 
   Size:        177     107      43      57      81     152     778     190

Cuts at:   1914    2706     329 
   Size:        792    1555

  Fragments arranged by size: 

                1555     792     778     190     177     152     107      81
                  57      43
 
Bsp1286I G_dGCh'C

Cuts at:    329     506     541     613     656     713     794     946    1557 
   Size:        177      35      72      43      57      81     152     611

Cuts at:   1557    1650    1724    1914    1976    2706     329 
   Size:         93      74     190      62     730    1555

  Fragments arranged by size: 

                1555     730     611     190     177     152      93      81
                  74      72      62      57      43      35
 
BspEI T'CCGG_A

Cuts at:   3152    3152 
   Size:       3932
 
BspHI T'CATG_A

Cuts at:   3108    3108 
   Size:       3932
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    404     491     982    1196    1498    1879     404 
   Size:         87     491     214     302     381    2457

  Fragments arranged by size: 

                2457     491     381     302     214      87
 
BsrBI CCG'CTC

Cuts at:     50    2224    2278    3762    3927      50 
   Size:       2174      54    1484     165      55

  Fragments arranged by size: 

                2174    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1844    1844 
   Size:       3932
 
BsrFI r'CCGG_y

Cuts at:    239     466     760    1930    2111    2320     239 
   Size:        227     294    1170     181     209    1851

  Fragments arranged by size: 

                1851    1170     294     227     209     181
 
BsrGI T'GTAC_A

Cuts at:    365     365 
   Size:       3932
 
BssHII G'CGCG_C

Cuts at:    442     892     909     442 
   Size:        450      17    3465

  Fragments arranged by size: 

                3465     450      17
 
BssSI C'ACGA_G

Cuts at:   2203    2561    2203 
   Size:        358    3574
 
BstAPI GCAn_nnn'nTGC

Cuts at:    324     408    1309     324 
   Size:         84     901    2947

  Fragments arranged by size: 

                2947     901      84
 
BstBI TT'CG_AA

Cuts at:   2293    2293 
   Size:       3932
 
BstEII G'GTnAC_C

Cuts at:    723     723 
   Size:       3932
 
BstXI CCAn_nnnn'nTGG

Cuts at:    231     592     231 
   Size:        361    3571
 
BstYI r'GATC_y

Cuts at:      1     957    1424    1447    1782    2028    3040    3126    3138 
   Size:        956     467      23     335     246    1012      86      12

Cuts at:   3138    3484       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     956     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    218     591     878     218 
   Size:        373     287    3272

  Fragments arranged by size: 

                3272     373     287
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    741    1882    2063    2226     741 
   Size:       1141     181     163    2447

  Fragments arranged by size: 

                2447    1141     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1175    1175 
   Size:       3932
 
DraI TTT'AAA

Cuts at:    931     931 
   Size:       3932
 
DraIII CAC_nnn'GTG

Cuts at:    324     501     731     324 
   Size:        177     230    3525

  Fragments arranged by size: 

                3525     230     177
 
DrdI GACnn_nn'nnGTC

Cuts at:   1638    2496    1638 
   Size:        858    3074
 
EaeI y'GGCC_r

Cuts at:    191     221     383     579    1517    1691    2082    2109    3454 
   Size:         30     162     196     938     174     391      27    1345

Cuts at:   3454     191 
   Size:        669

  Fragments arranged by size: 

                1345     938     669     391     196     174     162      30
                  27
 
EagI C'GGCC_G

Cuts at:    383     579    1517     383 
   Size:        196     938    2798

  Fragments arranged by size: 

                2798     938     196
 
EarI CTCTTCn'nnn_

Cuts at:   1955    2165    1955 
   Size:        210    3722
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    855    2140    2450    2596    3742     855 
   Size:       1285     310     146    1146    1045

  Fragments arranged by size: 

                1285    1146    1045     310     146
 
EcoICRI GAG'CTC

Cuts at:    944     944 
   Size:       3932
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1757    2189    2936    3204    1757 
   Size:        432     747     268    2485

  Fragments arranged by size: 

                2485     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    831     872    1071     831 
   Size:         41     199    3692

  Fragments arranged by size: 

                3692     199      41
 
EcoRI G'AATT_C

Cuts at:    936     936 
   Size:       3932
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1951    1951 
   Size:       3932
 
FspI TGC'GCA

Cuts at:    458    1713     458 
   Size:       1255    2677
 
HaeII r_GCGC'y

Cuts at:    253     473     552     777     825    1366    1614    2636    3615 
   Size:        220      79     225      48     541     248    1022     979

Cuts at:   3615     253 
   Size:        570

  Fragments arranged by size: 

                1022     979     570     541     248     225     220      79
                  48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1774    1804    1806    1836    1774 
   Size:         30       2      30    3870

  Fragments arranged by size: 

                3870      30      30       2
 
HincII GTy'rAC

Cuts at:    267     971     267 
   Size:        704    3228
 
KasI G'GCGC_C

Cuts at:    469     773    1362    1610     469 
   Size:        304     589     248    2791

  Fragments arranged by size: 

                2791     589     304     248
 
KpnI G_GTAC'C

Cuts at:    218     952     218 
   Size:        734    3198
 
MfeI C'AATT_G

Cuts at:    158     158 
   Size:       3932
 
MluI A'CGCG_T

Cuts at:    638    3365     638 
   Size:       2727    1205
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2603    2787    2603 
   Size:        184    3748
 
MscI TGG'CCA

Cuts at:    193     223    1693     193 
   Size:         30    1470    2432

  Fragments arranged by size: 

                2432    1470      30
 
MslI CAynn'nnrTG

Cuts at:    229     346     373    1921    2048     229 
   Size:        117      27    1548     127    2113

  Fragments arranged by size: 

                2113    1548     127     117      27
 
MspA1I CmG'CkG

Cuts at:    880    1043    1357    1717    2730    2975     880 
   Size:        163     314     360    1013     245    1837

  Fragments arranged by size: 

                1837    1013     360     314     245     163
 
NaeI GCC'GGC

Cuts at:    468     762    2113     468 
   Size:        294    1351    2287

  Fragments arranged by size: 

                2287    1351     294
 
NarI GG'CG_CC

Cuts at:    470     774    1363    1611     470 
   Size:        304     589     248    2791

  Fragments arranged by size: 

                2791     589     304     248
 
NcoI C'CATG_G

Cuts at:    591     591 
   Size:       3932
 
NgoMIV G'CCGG_C

Cuts at:    466     760    2111     466 
   Size:        294    1351    2287

  Fragments arranged by size: 

                2287    1351     294
 
NotI GC'GGCC_GC

Cuts at:    383     383 
   Size:       3932
 
NspI r_CATG'y

Cuts at:    738     985    1485    2392     738 
   Size:        247     500     907    2278

  Fragments arranged by size: 

                2278     907     500     247
 
PciI A'CATG_T

Cuts at:   2388    2388 
   Size:       3932
 
PmeI GTTT'AAAC

Cuts at:    931     931 
   Size:       3932
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3071    3103    3071 
   Size:         32    3900
 
PsiI TTA'TAA

Cuts at:    143     143 
   Size:       3932
 
PspXI vC'TCGA_Gb

Cuts at:    808     808 
   Size:       3932
 
PstI C_TGCA'G

Cuts at:    854     979    1664     854 
   Size:        125     685    3122

  Fragments arranged by size: 

                3122     685     125
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3932
 
PvuII CAG'CTG

Cuts at:   1357    1717    1357 
   Size:        360    3572
 
RsrII CG'GwC_CG

Cuts at:   2127    2127 
   Size:       3932
 
SacI G_AGCT'C

Cuts at:    946     946 
   Size:       3932
 
SacII CC_GC'GG

Cuts at:    881     881 
   Size:       3932
 
SalI G'TCGA_C

Cuts at:    969     969 
   Size:       3932
 
SapI GCTCTTCn'nnn_

Cuts at:   1955    2165    1955 
   Size:        210    3722
 
SbfI CC_TGCA'GG

Cuts at:    979     979 
   Size:       3932
 
ScaI AGT'ACT

Cuts at:   3449    3449 
   Size:       3932
 
SexAI A'CCwGG_T

Cuts at:    497     497 
   Size:       3932
 
SfcI C'TryA_G

Cuts at:     33     850     975    1660    2653    2844      33 
   Size:        817     125     685     993     191    1121

  Fragments arranged by size: 

                1121     993     817     685     191     125
 
SfoI GGC'GCC

Cuts at:    471     775    1364    1612     471 
   Size:        304     589     248    2791

  Fragments arranged by size: 

                2791     589     304     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3932
 
SgrAI Cr'CCGG_yG

Cuts at:    466     466 
   Size:       3932
 
SmaI CCC'GGG

Cuts at:    954     954 
   Size:       3932
 
SmlI C'TyrA_G

Cuts at:    514     808    1087    2002    2494    2756     514 
   Size:        294     279     915     492     262    1690

  Fragments arranged by size: 

                1690     915     492     294     279     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3932
 
SphI G_CATG'C

Cuts at:    738     985    1485     738 
   Size:        247     500    3185

  Fragments arranged by size: 

                3185     500     247
 
StuI AGG'CCT

Cuts at:    806     806 
   Size:       3932
 
StyI C'CwwG_G

Cuts at:    179     591    1066    1420     179 
   Size:        412     475     354    2691

  Fragments arranged by size: 

                2691     475     412     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1515    1648    2107    2316    1515 
   Size:        133     459     209    3131

  Fragments arranged by size: 

                3131     459     209     133
 
TatI w'GTAC_w

Cuts at:    206     365    3447     206 
   Size:        159    3082     691

  Fragments arranged by size: 

                3082     691     159
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3449    3481    3449 
   Size:         32    3900
 
Tth111I GACn'n_nGTC

Cuts at:    262    1729     262 
   Size:       1467    2465
 
XbaI T'CTAG_A

Cuts at:    963     963 
   Size:       3932
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    186     231    1427     186 
   Size:         45    1196    2691

  Fragments arranged by size: 

                2691    1196      45
 
XhoI C'TCGA_G

Cuts at:    808     808 
   Size:       3932
 
XmaI C'CCGG_G

Cuts at:    952     952 
   Size:       3932
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI     PciI
     PmeI     PpiI     PsiI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII    BbvCI    BfrBI     BglI     BmtI     BpmI    BsaXI
    BseRI    BsiWI     BsmI    BsmBI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI     NheI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI   PspOMI     PsrI    SanDI     SfiI    SnaBI     SrfI     SspI
     SwaI     XmnI     ZraI
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