Restriction map for pF1KB8782
 (Linear) (Six-base) MAPSORT of: KIBB8782.seq  Check: 7683  from: 1  to: 819



KIBB8782.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

Acc65I G'GTAC_C

Cuts at:      0     106     819 
   Size:        106     713
 
AfeI AGC'GCT

Cuts at:      0     715     819 
   Size:        715     104
 
AflIII A'CryG_T

Cuts at:      0     530     819 
   Size:        530     289
 
ApaLI G'TGCA_C

Cuts at:      0     217     394     819 
   Size:        217     177     425

  Fragments arranged by size: 

                 425     217     177
 
AvaI C'yCGr_G

Cuts at:      0     538     700     819 
   Size:        538     162     119

  Fragments arranged by size: 

                 538     162     119
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      90     123     819 
   Size:         90      33     696

  Fragments arranged by size: 

                 696      90      33
 
BanI G'GyrC_C

Cuts at:      0     106     361     665     819 
   Size:        106     255     304     154

  Fragments arranged by size: 

                 304     255     154     106
 
BanII G_rGCy'C

Cuts at:      0     433     819 
   Size:        433     386
 
BbeI G_GCGC'C

Cuts at:      0     365     669     819 
   Size:        365     304     150

  Fragments arranged by size: 

                 365     304     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     216     819 
   Size:        216     603
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     328     362     579     613     615     649     819 
   Size:        328      34     217      34       2      34     170

  Fragments arranged by size: 

                 328     217     170      34      34      34       2
 
BlpI GC'TnA_GC

Cuts at:      0     427     519     819 
   Size:        427      92     300

  Fragments arranged by size: 

                 427     300      92
 
Bme1580I G_kGCm'C

Cuts at:      0     221     398     819 
   Size:        221     177     421

  Fragments arranged by size: 

                 421     221     177
 
BmgBI CAC'GTC

Cuts at:      0     222     819 
   Size:        222     597
 
Bpu10I CC'TnA_GC

Cuts at:      0     499     819 
   Size:        499     320
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     819 
   Size:        391     428
 
BsaHI Gr'CG_yC

Cuts at:      0     362     596     666     819 
   Size:        362     234      70     153

  Fragments arranged by size: 

                 362     234     153      70
 
BseYI C'CCAG_C

Cuts at:      0      39     819 
   Size:         39     780
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     361     819 
   Size:        322      39     458

  Fragments arranged by size: 

                 458     322      39
 
BsiEI CG_ry'CG

Cuts at:      0     278     474     819 
   Size:        278     196     345

  Fragments arranged by size: 

                 345     278     196
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     398     505     548     605     686     819 
   Size:        221     177     107      43      57      81     133

  Fragments arranged by size: 

                 221     177     133     107      81      57      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     398     433     505     548     605     686     819 
   Size:        221     177      35      72      43      57      81     133

  Fragments arranged by size: 

                 221     177     133      81      72      57      43      35
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296     383     819 
   Size:        296      87     436

  Fragments arranged by size: 

                 436     296      87
 
BsrFI r'CCGG_y

Cuts at:      0     131     358     652     819 
   Size:        131     227     294     167

  Fragments arranged by size: 

                 294     227     167     131
 
BsrGI T'GTAC_A

Cuts at:      0     257     819 
   Size:        257     562
 
BssHII G'CGCG_C

Cuts at:      0     334     784     801     819 
   Size:        334     450      17      18

  Fragments arranged by size: 

                 450     334      18      17
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     216     300     819 
   Size:        216      84     519

  Fragments arranged by size: 

                 519     216      84
 
BstEII G'GTnAC_C

Cuts at:      0     615     819 
   Size:        615     204
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123     484     819 
   Size:        123     361     335

  Fragments arranged by size: 

                 361     335     123
 
BtgI C'CryG_G

Cuts at:      0     110     483     770     819 
   Size:        110     373     287      49

  Fragments arranged by size: 

                 373     287     110      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     633     819 
   Size:        633     186
 
DraIII CAC_nnn'GTG

Cuts at:      0     216     393     623     819 
   Size:        216     177     230     196

  Fragments arranged by size: 

                 230     216     196     177
 
EaeI y'GGCC_r

Cuts at:      0      83     113     275     471     819 
   Size:         83      30     162     196     348

  Fragments arranged by size: 

                 348     196     162      83      30
 
EagI C'GGCC_G

Cuts at:      0     275     471     819 
   Size:        275     196     348

  Fragments arranged by size: 

                 348     275     196
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     747     819 
   Size:        747      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     723     764     819 
   Size:        723      41      55

  Fragments arranged by size: 

                 723      55      41
 
FspI TGC'GCA

Cuts at:      0     350     819 
   Size:        350     469
 
HaeII r_GCGC'y

Cuts at:      0     145     365     444     669     717     819 
   Size:        145     220      79     225      48     102

  Fragments arranged by size: 

                 225     220     145     102      79      48
 
HincII GTy'rAC

Cuts at:      0     159     819 
   Size:        159     660
 
KasI G'GCGC_C

Cuts at:      0     361     665     819 
   Size:        361     304     154

  Fragments arranged by size: 

                 361     304     154
 
KpnI G_GTAC'C

Cuts at:      0     110     819 
   Size:        110     709
 
MfeI C'AATT_G

Cuts at:      0      50     819 
   Size:         50     769
 
MluI A'CGCG_T

Cuts at:      0     530     819 
   Size:        530     289
 
MscI TGG'CCA

Cuts at:      0      85     115     819 
   Size:         85      30     704

  Fragments arranged by size: 

                 704      85      30
 
MslI CAynn'nnrTG

Cuts at:      0     121     238     265     819 
   Size:        121     117      27     554

  Fragments arranged by size: 

                 554     121     117      27
 
MspA1I CmG'CkG

Cuts at:      0     772     819 
   Size:        772      47
 
NaeI GCC'GGC

Cuts at:      0     360     654     819 
   Size:        360     294     165

  Fragments arranged by size: 

                 360     294     165
 
NarI GG'CG_CC

Cuts at:      0     362     666     819 
   Size:        362     304     153

  Fragments arranged by size: 

                 362     304     153
 
NcoI C'CATG_G

Cuts at:      0     483     819 
   Size:        483     336
 
NgoMIV G'CCGG_C

Cuts at:      0     358     652     819 
   Size:        358     294     167

  Fragments arranged by size: 

                 358     294     167
 
NotI GC'GGCC_GC

Cuts at:      0     275     819 
   Size:        275     544
 
NspI r_CATG'y

Cuts at:      0     630     819 
   Size:        630     189
 
PsiI TTA'TAA

Cuts at:      0      35     819 
   Size:         35     784
 
PspXI vC'TCGA_Gb

Cuts at:      0     700     819 
   Size:        700     119
 
PstI C_TGCA'G

Cuts at:      0     746     819 
   Size:        746      73
 
SacII CC_GC'GG

Cuts at:      0     773     819 
   Size:        773      46
 
SexAI A'CCwGG_T

Cuts at:      0     389     819 
   Size:        389     430
 
SfcI C'TryA_G

Cuts at:      0     742     819 
   Size:        742      77
 
SfoI GGC'GCC

Cuts at:      0     363     667     819 
   Size:        363     304     152

  Fragments arranged by size: 

                 363     304     152
 
SgrAI Cr'CCGG_yG

Cuts at:      0     358     819 
   Size:        358     461
 
SmlI C'TyrA_G

Cuts at:      0     406     700     819 
   Size:        406     294     119

  Fragments arranged by size: 

                 406     294     119
 
SphI G_CATG'C

Cuts at:      0     630     819 
   Size:        630     189
 
StuI AGG'CCT

Cuts at:      0     698     819 
   Size:        698     121
 
StyI C'CwwG_G

Cuts at:      0      71     483     819 
   Size:         71     412     336

  Fragments arranged by size: 

                 412     336      71
 
TatI w'GTAC_w

Cuts at:      0      98     257     819 
   Size:         98     159     562

  Fragments arranged by size: 

                 562     159      98
 
Tth111I GACn'n_nGTC

Cuts at:      0     154     819 
   Size:        154     665
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78     123     819 
   Size:         78      45     696

  Fragments arranged by size: 

                 696      78      45
 
XhoI C'TCGA_G

Cuts at:      0     700     819 
   Size:        700     119
 

 Enzymes that do cut:

   Acc65I     AfeI   AflIII    ApaLI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI     BcgI     BlpI Bme1580I    BmgBI   Bpu10I    BpuEI
    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI     BtgI    BtgZI   DraIII
     EaeI     EagI     EciI EcoO109I     FspI    HaeII   HincII     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NspI     PsiI    PspXI     PstI    SacII
    SexAI     SfcI     SfoI    SgrAI     SmlI     SphI     StuI     StyI
     TatI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI     DrdI     EarI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII     HpaI
     MmeI     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SgfI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     TstI     XbaI     XmaI     XmnI     ZraI
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