Restriction map for pF1KB8777
 (Circular) (Six-base) MAPSORT of: pF1KB8777.seq  Check: 5721  from: 1  to: 3884



pF1KB8777  3884 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    374     374 
   Size:       3884
 
AccI GT'mk_AC

Cuts at:    922     922 
   Size:       3884
 
Acc65I G'GTAC_C

Cuts at:    900     900 
   Size:       3884
 
AclI AA'CG_TT

Cuts at:   3724    3724 
   Size:       3884
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    256     334     607     847    1709    2141    2888    3156     256 
   Size:         78     273     240     862     432     747     268     984

  Fragments arranged by size: 

                 984     862     747     432     273     268     240      78
            
 
AflIII A'CryG_T

Cuts at:   1236    2340    3317    1236 
   Size:       1104     977    1803

  Fragments arranged by size: 

                1803    1104     977
 
AgeI A'CCGG_T

Cuts at:   2272    2272 
   Size:       3884
 
AleI CACnn'nnGTG

Cuts at:    631     718     631 
   Size:         87    3797
 
AlwNI CAG_nnn'CTG

Cuts at:    241     366     672     692    1189    2756     241 
   Size:        125     306      20     497    1567    1369

  Fragments arranged by size: 

                1567    1369     497     306     125      20
 
ApaI G_GGCC'C

Cuts at:    878     878 
   Size:       3884
 
ApaLI G'TGCA_C

Cuts at:   2654    2654 
   Size:       3884
 
ApoI r'AATT_y

Cuts at:    888    3237    3493     888 
   Size:       2349     256    1279

  Fragments arranged by size: 

                2349    1279     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3884
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3884
 
AvaI C'yCGr_G

Cuts at:    155     283     870     904     155 
   Size:        128     587      34    3135

  Fragments arranged by size: 

                3135     587     128      34
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    517     550     517 
   Size:         33    3851
 
BamHI G'GATC_C

Cuts at:    488     909     488 
   Size:        421    3463
 
BanI G'GyrC_C

Cuts at:    112     196     221     682     812     900    1314    1562    1597 
   Size:         84      25     461     130      88     414     248      35

Cuts at:   1597     112 
   Size:       2399

  Fragments arranged by size: 

                2399     461     414     248     130      88      84      35
                  25
 
BanII G_rGCy'C

Cuts at:    878     898    1928     878 
   Size:         20    1030    2834

  Fragments arranged by size: 

                2834    1030      20
 
BbeI G_GCGC'C

Cuts at:    200    1318    1566     200 
   Size:       1118     248    2518

  Fragments arranged by size: 

                2518    1118     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    243     243 
   Size:       3884
 
BciVI GTATCCnnnnn_n'

Cuts at:   1782    2543    3884    1782 
   Size:        761    1341    1782

  Fragments arranged by size: 

                1782    1341     761
 
BclI T'GATC_A

Cuts at:   1404    1404 
   Size:       3884
 
BglI GCCn_nnn'nGGC

Cuts at:    409     409 
   Size:       3884
 
BglII A'GATC_T

Cuts at:    332    1399    3436     332 
   Size:       1067    2037     780

  Fragments arranged by size: 

                2037    1067     780
 
BlpI GC'TnA_GC

Cuts at:    996     996 
   Size:       3884
 
Bme1580I G_kGCm'C

Cuts at:    115     226     878    1509    1602    2658     115 
   Size:        111     652     631      93    1056    1341

  Fragments arranged by size: 

                1341    1056     652     631     111      93
 
BmgBI CAC'GTC

Cuts at:    720     720 
   Size:       3884
 
BmrI ACTGGGnnnn_n'

Cuts at:   1096    1177    1512    3656    1096 
   Size:         81     335    2144    1324

  Fragments arranged by size: 

                2144    1324     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2251    2251 
   Size:       3884
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    838     838 
   Size:       3884
 
Bpu10I CC'TnA_GC

Cuts at:    376     376 
   Size:       3884
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1060    1939    2431    2729    1060 
   Size:        879     492     298    2215

  Fragments arranged by size: 

                2215     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    153    3588     153 
   Size:       3435     449
 
BsaAI yAC'GTr

Cuts at:   1237    1237 
   Size:       3884
 
BsaBI GATnn'nnATC

Cuts at:      6    1398    3435       6 
   Size:       1392    2037     455

  Fragments arranged by size: 

                2037    1392     455
 
BsaHI Gr'CG_yC

Cuts at:    197     500    1315    1563    2265    3761     197 
   Size:        303     815     248     702    1496     320

  Fragments arranged by size: 

                1496     815     702     320     303     248
 
BsaWI w'CCGG_w

Cuts at:   1073    1594    2272    2546    2693    3104    1073 
   Size:        521     678     274     147     411    1853

  Fragments arranged by size: 

                1853     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    319     349     562     592     319 
   Size:         30     213      30    3611

  Fragments arranged by size: 

                3611     213      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    166     655     166 
   Size:        489    3395
 
BseYI C'CCAG_C

Cuts at:    838    1309    2067    2644    3140     838 
   Size:        471     758     577     496    1582

  Fragments arranged by size: 

                1582     758     577     496     471
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    172     352     532     655     172 
   Size:        180     180     123    3401

  Fragments arranged by size: 

                3401     180     180     123
 
BsiEI CG_ry'CG

Cuts at:    104    1426    1472    2680     104 
   Size:       1322      46    1208    1308

  Fragments arranged by size: 

                1322    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    311     898    1676    1866    2658     311 
   Size:        587     778     190     792    1537

  Fragments arranged by size: 

                1537     792     778     587     190
 
Bsp1286I G_dGCh'C

Cuts at:    115     226     311     878     898    1509    1602    1676    1866 
   Size:        111      85     567      20     611      93      74     190

Cuts at:   1866    1928    2658     115 
   Size:         62     730    1341

  Fragments arranged by size: 

                1341     730     611     567     190     111      93      85
                  74      62      20
 
BspEI T'CCGG_A

Cuts at:   3104    3104 
   Size:       3884
 
BspHI T'CATG_A

Cuts at:   3060    3060 
   Size:       3884
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    374     934    1148    1450    1831     374 
   Size:        560     214     302     381    2427

  Fragments arranged by size: 

                2427     560     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2176    2230    3714    3879      50 
   Size:       2126      54    1484     165      55

  Fragments arranged by size: 

                2126    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1796    1796 
   Size:       3884
 
BsrFI r'CCGG_y

Cuts at:    218     371    1882    2063    2272     218 
   Size:        153    1511     181     209    1830

  Fragments arranged by size: 

                1830    1511     209     181     153
 
BssSI C'ACGA_G

Cuts at:   2155    2513    2155 
   Size:        358    3526
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1261    1261 
   Size:       3884
 
BstBI TT'CG_AA

Cuts at:   2245    2245 
   Size:       3884
 
BstEII G'GTnAC_C

Cuts at:    732     732 
   Size:       3884
 
BstXI CCAn_nnnn'nTGG

Cuts at:    720     720 
   Size:       3884
 
BstYI r'GATC_y

Cuts at:      1     332     387     488     909    1376    1399    1734    1980 
   Size:        331      55     101     421     467      23     335     246

Cuts at:   1980    2992    3078    3090    3436       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     421     346     335     331     246
                 101      86      55      23      12
 
BtgI C'CryG_G

Cuts at:    107     755     107 
   Size:        648    3236
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1834    2015    2178    1834 
   Size:        181     163    3540

  Fragments arranged by size: 

                3540     181     163
 
BtsI GCAGTG_nn'

Cuts at:    549    1127     549 
   Size:        578    3306
 
DraI TTT'AAA

Cuts at:    883     883 
   Size:       3884
 
DrdI GACnn_nn'nnGTC

Cuts at:   1590    2448    1590 
   Size:        858    3026
 
EaeI y'GGCC_r

Cuts at:    296     440     722    1469    1643    2034    2061    3406     296 
   Size:        144     282     747     174     391      27    1345     774

  Fragments arranged by size: 

                1345     774     747     391     282     174     144      27
            
 
EagI C'GGCC_G

Cuts at:   1469    1469 
   Size:       3884
 
EarI CTCTTCn'nnn_

Cuts at:    175     184    1907    2117     175 
   Size:          9    1723     210    1942

  Fragments arranged by size: 

                1942    1723     210       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    411    2092    2402    2548    3694     411 
   Size:       1681     310     146    1146     601

  Fragments arranged by size: 

                1681    1146     601     310     146
 
EcoICRI GAG'CTC

Cuts at:    896     896 
   Size:       3884
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    256     334     607     838     847    1709    2141    2888    3156 
   Size:         78     273     231       9     862     432     747     268

Cuts at:   3156     256 
   Size:        984

  Fragments arranged by size: 

                 984     862     747     432     273     268     231      78
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:    274     568     274 
   Size:        294    3590
 
EcoO109I rG'GnC_Cy

Cuts at:    267     279     353     447     573     858     874    1023     267 
   Size:         12      74      94     126     285      16     149    3128

  Fragments arranged by size: 

                3128     285     149     126      94      74      16      12
            
 
EcoRI G'AATT_C

Cuts at:    888     888 
   Size:       3884
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1903    1903 
   Size:       3884
 
FspI TGC'GCA

Cuts at:   1665    1665 
   Size:       3884
 
HaeII r_GCGC'y

Cuts at:    200     521     845    1318    1566    2588    3567     200 
   Size:        321     324     473     248    1022     979     517

  Fragments arranged by size: 

                1022     979     517     473     324     321     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    586     618    1726    1756    1758    1788     586 
   Size:         32    1108      30       2      30    2682

  Fragments arranged by size: 

                2682    1108      32      30      30       2
 
HincII GTy'rAC

Cuts at:    923     923 
   Size:       3884
 
KasI G'GCGC_C

Cuts at:    196    1314    1562     196 
   Size:       1118     248    2518

  Fragments arranged by size: 

                2518    1118     248
 
KpnI G_GTAC'C

Cuts at:    904     904 
   Size:       3884
 
MluI A'CGCG_T

Cuts at:   3317    3317 
   Size:       3884
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2555    2739    2555 
   Size:        184    3700
 
MscI TGG'CCA

Cuts at:    724    1645     724 
   Size:        921    2963
 
MslI CAynn'nnrTG

Cuts at:    631     718    1873    2000     631 
   Size:         87    1155     127    2515

  Fragments arranged by size: 

                2515    1155     127      87
 
MspA1I CmG'CkG

Cuts at:    995    1309    1669    2682    2927     995 
   Size:        314     360    1013     245    1952

  Fragments arranged by size: 

                1952    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2065    2065 
   Size:       3884
 
NarI GG'CG_CC

Cuts at:    197    1315    1563     197 
   Size:       1118     248    2518

  Fragments arranged by size: 

                2518    1118     248
 
NcoI C'CATG_G

Cuts at:    107     755     107 
   Size:        648    3236
 
NgoMIV G'CCGG_C

Cuts at:   2063    2063 
   Size:       3884
 
NspI r_CATG'y

Cuts at:    937    1437    2344     937 
   Size:        500     907    2477

  Fragments arranged by size: 

                2477     907     500
 
PasI CC'CwG_GG

Cuts at:    504     504 
   Size:       3884
 
PciI A'CATG_T

Cuts at:   2340    2340 
   Size:       3884
 
PfoI T'CCnGG_A

Cuts at:    344     344 
   Size:       3884
 
PmeI GTTT'AAAC

Cuts at:    883     883 
   Size:       3884
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3023    3055    3023 
   Size:         32    3852
 
PpuMI rG'GwC_Cy

Cuts at:    279     858     279 
   Size:        579    3305
 
PspOMI G'GGCC_C

Cuts at:    874     874 
   Size:       3884
 
PstI C_TGCA'G

Cuts at:    259     569     697     931    1616     259 
   Size:        310     128     234     685    2527

  Fragments arranged by size: 

                2527     685     310     234     128
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3884
 
PvuII CAG'CTG

Cuts at:   1309    1669    1309 
   Size:        360    3524
 
RsrII CG'GwC_CG

Cuts at:   2079    2079 
   Size:       3884
 
SacI G_AGCT'C

Cuts at:    898     898 
   Size:       3884
 
SalI G'TCGA_C

Cuts at:    921     921 
   Size:       3884
 
SapI GCTCTTCn'nnn_

Cuts at:   1907    2117    1907 
   Size:        210    3674
 
SbfI CC_TGCA'GG

Cuts at:    569     931     569 
   Size:        362    3522
 
ScaI AGT'ACT

Cuts at:   3401    3401 
   Size:       3884
 
SfcI C'TryA_G

Cuts at:     33     255     565     693     927    1612    2605    2796      33 
   Size:        222     310     128     234     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     310     234     222     191     128
            
 
SfoI GGC'GCC

Cuts at:    198    1316    1564     198 
   Size:       1118     248    2518

  Fragments arranged by size: 

                2518    1118     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3884
 
SmaI CCC'GGG

Cuts at:    157     872     906     157 
   Size:        715      34    3135

  Fragments arranged by size: 

                3135     715      34
 
SmlI C'TyrA_G

Cuts at:   1039    1954    2446    2708    1039 
   Size:        915     492     262    2215

  Fragments arranged by size: 

                2215     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3884
 
SphI G_CATG'C

Cuts at:    937    1437     937 
   Size:        500    3384
 
StyI C'CwwG_G

Cuts at:    107     494     755     854    1018    1372     107 
   Size:        387     261      99     164     354    2619

  Fragments arranged by size: 

                2619     387     354     261     164      99
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1467    1600    2059    2268    1467 
   Size:        133     459     209    3083

  Fragments arranged by size: 

                3083     459     209     133
 
TatI w'GTAC_w

Cuts at:    776    3399     776 
   Size:       2623    1261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    562     594    3401    3433     562 
   Size:         32    2807      32    1013

  Fragments arranged by size: 

                2807    1013      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1681    1681 
   Size:       3884
 
XbaI T'CTAG_A

Cuts at:    915     915 
   Size:       3884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    501    1379     501 
   Size:        878    3006
 
XmaI C'CCGG_G

Cuts at:    155     870     904     155 
   Size:        715      34    3135

  Fragments arranged by size: 

                3135     715      34
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI    AvrII
    BbvCI     BcgI    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmlI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com