Restriction map for pF1KB8770
 (Linear) (Six-base) MAPSORT of: KIBB8770.seq  Check: 5355  from: 1  to: 702



KIBB8770.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AflII C'TTAA_G

Cuts at:      0     442     702 
   Size:        442     260
 
AflIII A'CryG_T

Cuts at:      0     692     702 
   Size:        692      10
 
ApaLI G'TGCA_C

Cuts at:      0     252     702 
   Size:        252     450
 
ApoI r'AATT_y

Cuts at:      0     271     702 
   Size:        271     431
 
AvaI C'yCGr_G

Cuts at:      0     160     583     702 
   Size:        160     423     119

  Fragments arranged by size: 

                 423     160     119
 
BanII G_rGCy'C

Cuts at:      0     658     702 
   Size:        658      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     628     702 
   Size:        628      74
 
BbvCI CC'TCA_GC

Cuts at:      0     676     702 
   Size:        676      26
 
BglII A'GATC_T

Cuts at:      0       5     702 
   Size:          5     697
 
Bme1580I G_kGCm'C

Cuts at:      0     256     702 
   Size:        256     446
 
BmgBI CAC'GTC

Cuts at:      0     695     702 
   Size:        695       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     252     702 
   Size:        252     450
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     702 
   Size:        652      50
 
Bpu10I CC'TnA_GC

Cuts at:      0      47     676     702 
   Size:         47     629      26

  Fragments arranged by size: 

                 629      47      26
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     301     702 
   Size:        226      75     401

  Fragments arranged by size: 

                 401     226      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     111     526     702 
   Size:        111     415     176

  Fragments arranged by size: 

                 415     176     111
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     564     594     702 
   Size:        564      30     108

  Fragments arranged by size: 

                 564     108      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     702 
   Size:        241     461
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     435     648     702 
   Size:        435     213      54

  Fragments arranged by size: 

                 435     213      54
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     256     518     658     702 
   Size:         56     200     262     140      44

  Fragments arranged by size: 

                 262     200     140      56      44
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     256     518     658     702 
   Size:         56     200     262     140      44

  Fragments arranged by size: 

                 262     200     140      56      44
 
BsrFI r'CCGG_y

Cuts at:      0     289     702 
   Size:        289     413
 
BstYI r'GATC_y

Cuts at:      0       5     702 
   Size:          5     697
 
BtgI C'CryG_G

Cuts at:      0     566     702 
   Size:        566     136
 
BtsI GCAGTG_nn'

Cuts at:      0     560     702 
   Size:        560     142
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     447     482     702 
   Size:        447      35     220

  Fragments arranged by size: 

                 447     220      35
 
EaeI y'GGCC_r

Cuts at:      0     569     702 
   Size:        569     133
 
EcoICRI GAG'CTC

Cuts at:      0     656     702 
   Size:        656      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     702 
   Size:        652      50
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      61     702 
   Size:         61     641
 
EcoO109I rG'GnC_Cy

Cuts at:      0     597     702 
   Size:        597     105
 
MfeI C'AATT_G

Cuts at:      0     259     702 
   Size:        259     443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     702 
   Size:        322     380
 
MscI TGG'CCA

Cuts at:      0     571     702 
   Size:        571     131
 
MspA1I CmG'CkG

Cuts at:      0     397     702 
   Size:        397     305
 
NaeI GCC'GGC

Cuts at:      0     291     702 
   Size:        291     411
 
NcoI C'CATG_G

Cuts at:      0     566     702 
   Size:        566     136
 
NgoMIV G'CCGG_C

Cuts at:      0     289     702 
   Size:        289     413
 
PasI CC'CwG_GG

Cuts at:      0     509     702 
   Size:        509     193
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     567     702 
   Size:        567     135
 
PpuMI rG'GwC_Cy

Cuts at:      0     597     702 
   Size:        597     105
 
PstI C_TGCA'G

Cuts at:      0     205     702 
   Size:        205     497
 
PvuII CAG'CTG

Cuts at:      0     397     702 
   Size:        397     305
 
SacI G_AGCT'C

Cuts at:      0     658     702 
   Size:        658      44
 
SfcI C'TryA_G

Cuts at:      0       9     201     378     702 
   Size:          9     192     177     324

  Fragments arranged by size: 

                 324     192     177       9
 
SmaI CCC'GGG

Cuts at:      0     162     702 
   Size:        162     540
 
SmlI C'TyrA_G

Cuts at:      0     241     316     442     702 
   Size:        241      75     126     260

  Fragments arranged by size: 

                 260     241     126      75
 
StyI C'CwwG_G

Cuts at:      0     566     702 
   Size:        566     136
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     151     702 
   Size:        151     551
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     564     596     702 
   Size:        564      32     106

  Fragments arranged by size: 

                 564     106      32
 
XmaI C'CCGG_G

Cuts at:      0     160     702 
   Size:        160     542
 

 Enzymes that do cut:

    AflII   AflIII    ApaLI     ApoI     AvaI    BanII     BbsI    BbvCI
    BglII Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BsrFI    BstYI     BtgI
     BtsI    CspCI     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I     MfeI
     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI
    PpuMI     PstI    PvuII     SacI     SfcI     SmaI     SmlI     StyI
    TaqII     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MslI     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com