Restriction map for pF1KB8763
 (Circular) (Six-base) MAPSORT of: pF1KB8763.seq  Check: 7239  from: 1  to: 3746



pF1KB8763  3746 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    784     784 
   Size:       3746
 
Acc65I G'GTAC_C

Cuts at:    762     762 
   Size:       3746
 
AclI AA'CG_TT

Cuts at:   3586    3586 
   Size:       3746
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1571    2003    2750    3018    1571 
   Size:        432     747     268    2299

  Fragments arranged by size: 

                2299     747     432     268
 
AflIII A'CryG_T

Cuts at:   1098    2202    3179    1098 
   Size:       1104     977    1665

  Fragments arranged by size: 

                1665    1104     977
 
AgeI A'CCGG_T

Cuts at:   2134    2134 
   Size:       3746
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    505     505 
   Size:       3746
 
AlwNI CAG_nnn'CTG

Cuts at:    636    1051    2618     636 
   Size:        415    1567    1764

  Fragments arranged by size: 

                1764    1567     415
 
ApaI G_GGCC'C

Cuts at:    356     600     356 
   Size:        244    3502
 
ApaLI G'TGCA_C

Cuts at:    364     490    2516     364 
   Size:        126    2026    1594

  Fragments arranged by size: 

                2026    1594     126
 
ApoI r'AATT_y

Cuts at:    750    3099    3355     750 
   Size:       2349     256    1141

  Fragments arranged by size: 

                2349    1141     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3746
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3746
 
AvaI C'yCGr_G

Cuts at:    468     766     468 
   Size:        298    3448
 
BamHI G'GATC_C

Cuts at:    771     771 
   Size:       3746
 
BanI G'GyrC_C

Cuts at:    403     576     762    1176    1424    1459     403 
   Size:        173     186     414     248      35    2690

  Fragments arranged by size: 

                2690     414     248     186     173      35
 
BanII G_rGCy'C

Cuts at:    164     356     398     600     724     760    1790     164 
   Size:        192      42     202     124      36    1030    2120

  Fragments arranged by size: 

                2120    1030     202     192     124      42      36
 
BbeI G_GCGC'C

Cuts at:    580    1180    1428     580 
   Size:        600     248    2898

  Fragments arranged by size: 

                2898     600     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1644    2405    3746    1644 
   Size:        761    1341    1644

  Fragments arranged by size: 

                1644    1341     761
 
BclI T'GATC_A

Cuts at:   1266    1266 
   Size:       3746
 
BglI GCCn_nnn'nGGC

Cuts at:    168     168 
   Size:       3746
 
BglII A'GATC_T

Cuts at:   1261    3298    1261 
   Size:       2037    1709
 
BlpI GC'TnA_GC

Cuts at:    858     858 
   Size:       3746
 
Bme1580I G_kGCm'C

Cuts at:    235     356     368     406     494     600    1371    1464    2520 
   Size:        121      12      38      88     106     771      93    1056

Cuts at:   2520     235 
   Size:       1461

  Fragments arranged by size: 

                1461    1056     771     121     106      93      88      38
                  12
 
BmrI ACTGGGnnnn_n'

Cuts at:    357     958    1039    1374    3518     357 
   Size:        601      81     335    2144     585

  Fragments arranged by size: 

                2144     601     585     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2113    2113 
   Size:       3746
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    580     580 
   Size:       3746
 
Bpu10I CC'TnA_GC

Cuts at:    358     451     718     358 
   Size:         93     267    3386

  Fragments arranged by size: 

                3386     267      93
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    922    1801    2293    2591     922 
   Size:        879     492     298    2077

  Fragments arranged by size: 

                2077     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3450    3450 
   Size:       3746
 
BsaAI yAC'GTr

Cuts at:    570    1099     570 
   Size:        529    3217
 
BsaBI GATnn'nnATC

Cuts at:      6    1260    3297       6 
   Size:       1254    2037     455

  Fragments arranged by size: 

                2037    1254     455
 
BsaHI Gr'CG_yC

Cuts at:    332     577    1177    1425    2127    3623     332 
   Size:        245     600     248     702    1496     455

  Fragments arranged by size: 

                1496     702     600     455     248     245
 
BsaWI w'CCGG_w

Cuts at:    935    1456    2134    2408    2555    2966     935 
   Size:        521     678     274     147     411    1715

  Fragments arranged by size: 

                1715     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    706     736     706 
   Size:         30    3716
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    177     186     189     177 
   Size:          9       3    3734

  Fragments arranged by size: 

                3734       9       3
 
BseYI C'CCAG_C

Cuts at:    591    1171    1929    2506    3002     591 
   Size:        580     758     577     496    1335

  Fragments arranged by size: 

                1335     758     580     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     229     507     625    1288    1334    2542     104 
   Size:        125     278     118     663      46    1208    1308

  Fragments arranged by size: 

                1308    1208     663     278     125     118      46
 
BsiHKAI G_wGCw'C

Cuts at:    368     494     566     724     760    1538    1728    2520     368 
   Size:        126      72     158      36     778     190     792    1594

  Fragments arranged by size: 

                1594     792     778     190     158     126      72      36
            
 
Bsp1286I G_dGCh'C

Cuts at:    164     235     356     368     398     406     494     566     600 
   Size:         71     121      12      30       8      88      72      34

Cuts at:    600     724     760    1371    1464    1538    1728    1790    2520 
   Size:        124      36     611      93      74     190      62     730

Cuts at:   2520     164 
   Size:       1390

  Fragments arranged by size: 

                1390     730     611     190     124     121      93      88
                  74      72      71      62      36      34      30      12
                   8
 
BspEI T'CCGG_A

Cuts at:   2966    2966 
   Size:       3746
 
BspHI T'CATG_A

Cuts at:   2922    2922 
   Size:       3746
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    796    1010    1312    1693     796 
   Size:        214     302     381    2849

  Fragments arranged by size: 

                2849     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     381    2038    2092    3576    3741      50 
   Size:        331    1657      54    1484     165      55

  Fragments arranged by size: 

                1657    1484     331     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1658    1658 
   Size:       3746
 
BsrFI r'CCGG_y

Cuts at:    299     502    1744    1925    2134     299 
   Size:        203    1242     181     209    1911

  Fragments arranged by size: 

                1911    1242     209     203     181
 
BssHII G'CGCG_C

Cuts at:    217     312     320     387     217 
   Size:         95       8      67    3576

  Fragments arranged by size: 

                3576      95      67       8
 
BssSI C'ACGA_G

Cuts at:    376     477    2017    2375     376 
   Size:        101    1540     358    1747

  Fragments arranged by size: 

                1747    1540     358     101
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1123    1123 
   Size:       3746
 
BstBI TT'CG_AA

Cuts at:   2107    2107 
   Size:       3746
 
BstYI r'GATC_y

Cuts at:      1     771    1238    1261    1596    1842    2854    2940    2952 
   Size:        770     467      23     335     246    1012      86      12

Cuts at:   2952    3298       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     770     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     206     223     236     398     662     107 
   Size:         99      17      13     162     264    3191

  Fragments arranged by size: 

                3191     264     162      99      17      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1696    1877    2040    1696 
   Size:        181     163    3402

  Fragments arranged by size: 

                3402     181     163
 
BtsI GCAGTG_nn'

Cuts at:    989     989 
   Size:       3746
 
DraI TTT'AAA

Cuts at:    745     745 
   Size:       3746
 
DraIII CAC_nnn'GTG

Cuts at:    570     570 
   Size:       3746
 
DrdI GACnn_nn'nnGTC

Cuts at:    142    1452    2310     142 
   Size:       1310     858    1578

  Fragments arranged by size: 

                1578    1310     858
 
EaeI y'GGCC_r

Cuts at:    172     226     504     622     656    1331    1505    1896    1923 
   Size:         54     278     118      34     675     174     391      27

Cuts at:   1923    3268     172 
   Size:       1345     650

  Fragments arranged by size: 

                1345     675     650     391     278     174     118      54
                  34      27
 
EagI C'GGCC_G

Cuts at:    226     504     622    1331     226 
   Size:        278     118     709    2641

  Fragments arranged by size: 

                2641     709     278     118
 
EarI CTCTTCn'nnn_

Cuts at:    195     553    1769    1979     195 
   Size:        358    1216     210    1962

  Fragments arranged by size: 

                1962    1216     358     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1954    2264    2410    3556    1954 
   Size:        310     146    1146    2144

  Fragments arranged by size: 

                2144    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    722     758     722 
   Size:         36    3710
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    580    1571    2003    2750    3018     580 
   Size:        991     432     747     268    1308

  Fragments arranged by size: 

                1308     991     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    352     353     472     596     597     885     352 
   Size:          1     119     124       1     288    3213

  Fragments arranged by size: 

                3213     288     124     119       1       1
 
EcoRI G'AATT_C

Cuts at:    750     750 
   Size:       3746
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1765    1765 
   Size:       3746
 
FseI GG_CCGG'CC

Cuts at:    506     506 
   Size:       3746
 
FspI TGC'GCA

Cuts at:   1527    1527 
   Size:       3746
 
HaeII r_GCGC'y

Cuts at:    580     615    1180    1428    2450    3429     580 
   Size:         35     565     248    1022     979     897

  Fragments arranged by size: 

                1022     979     897     565     248      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1588    1618    1620    1650    1588 
   Size:         30       2      30    3684

  Fragments arranged by size: 

                3684      30      30       2
 
HincII GTy'rAC

Cuts at:    785     785 
   Size:       3746
 
KasI G'GCGC_C

Cuts at:    576    1176    1424     576 
   Size:        600     248    2898

  Fragments arranged by size: 

                2898     600     248
 
KpnI G_GTAC'C

Cuts at:    766     766 
   Size:       3746
 
MluI A'CGCG_T

Cuts at:   3179    3179 
   Size:       3746
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2417    2601    2417 
   Size:        184    3562
 
MscI TGG'CCA

Cuts at:    658    1507     658 
   Size:        849    2897
 
MslI CAynn'nnrTG

Cuts at:    487     518    1735    1862     487 
   Size:         31    1217     127    2371

  Fragments arranged by size: 

                2371    1217     127      31
 
MspA1I CmG'CkG

Cuts at:    208     225     238     432     603     627     857    1171    1531 
   Size:         17      13     194     171      24     230     314     360

Cuts at:   1531    2544    2789     208 
   Size:       1013     245    1165

  Fragments arranged by size: 

                1165    1013     360     314     245     230     194     171
                  24      17      13
 
NaeI GCC'GGC

Cuts at:    504    1927     504 
   Size:       1423    2323
 
NarI GG'CG_CC

Cuts at:    577    1177    1425     577 
   Size:        600     248    2898

  Fragments arranged by size: 

                2898     600     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3746
 
NgoMIV G'CCGG_C

Cuts at:    502    1925     502 
   Size:       1423    2323
 
NotI GC'GGCC_GC

Cuts at:    622     622 
   Size:       3746
 
NspI r_CATG'y

Cuts at:    591     799    1299    2206     591 
   Size:        208     500     907    2131

  Fragments arranged by size: 

                2131     907     500     208
 
PciI A'CATG_T

Cuts at:   2202    2202 
   Size:       3746
 
PfoI T'CCnGG_A

Cuts at:    326     326 
   Size:       3746
 
PmeI GTTT'AAAC

Cuts at:    745     745 
   Size:       3746
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2885    2917    2885 
   Size:         32    3714
 
PpuMI rG'GwC_Cy

Cuts at:    472     472 
   Size:       3746
 
PspOMI G'GGCC_C

Cuts at:    352     596     352 
   Size:        244    3502
 
PstI C_TGCA'G

Cuts at:    632     793    1478     632 
   Size:        161     685    2900

  Fragments arranged by size: 

                2900     685     161
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3746
 
PvuII CAG'CTG

Cuts at:   1171    1531    1171 
   Size:        360    3386
 
RsrII CG'GwC_CG

Cuts at:   1941    1941 
   Size:       3746
 
SacI G_AGCT'C

Cuts at:    724     760     724 
   Size:         36    3710
 
SacII CC_GC'GG

Cuts at:    209     226     239     209 
   Size:         17      13    3716

  Fragments arranged by size: 

                3716      17      13
 
SalI G'TCGA_C

Cuts at:    783     783 
   Size:       3746
 
SanDI GG'GwC_CC

Cuts at:    472     472 
   Size:       3746
 
SapI GCTCTTCn'nnn_

Cuts at:   1769    1979    1769 
   Size:        210    3536
 
SbfI CC_TGCA'GG

Cuts at:    793     793 
   Size:       3746
 
ScaI AGT'ACT

Cuts at:   3263    3263 
   Size:       3746
 
SexAI A'CCwGG_T

Cuts at:    122     122 
   Size:       3746
 
SfcI C'TryA_G

Cuts at:     33     628     789    1474    2467    2658      33 
   Size:        595     161     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     595     191     161
 
SfoI GGC'GCC

Cuts at:    578    1178    1426     578 
   Size:        600     248    2898

  Fragments arranged by size: 

                2898     600     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3746
 
SmaI CCC'GGG

Cuts at:    768     768 
   Size:       3746
 
SmlI C'TyrA_G

Cuts at:    901    1816    2308    2570     901 
   Size:        915     492     262    2077

  Fragments arranged by size: 

                2077     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3746
 
SphI G_CATG'C

Cuts at:    591     799    1299     591 
   Size:        208     500    3038

  Fragments arranged by size: 

                3038     500     208
 
StyI C'CwwG_G

Cuts at:    107     880    1234     107 
   Size:        773     354    2619

  Fragments arranged by size: 

                2619     773     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1329    1462    1921    2130    1329 
   Size:        133     459     209    2945

  Fragments arranged by size: 

                2945     459     209     133
 
TatI w'GTAC_w

Cuts at:   3261    3261 
   Size:       3746
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3263    3295    3263 
   Size:         32    3714
 
Tth111I GACn'n_nGTC

Cuts at:   1543    1543 
   Size:       3746
 
XbaI T'CTAG_A

Cuts at:    777     777 
   Size:       3746
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    324    1241     324 
   Size:        917    2829
 
XmaI C'CCGG_G

Cuts at:    766     766 
   Size:       3746
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NspI     PciI     PfoI     PmeI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI    EcoNI    EcoRV    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PshAI     PsiI    PspXI     PsrI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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