Restriction map for pF1KB8737
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MaeI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NciI        NcoI        NdeI        
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AlwI          186  GATCC                GGATC(4/5)
AscI          139  GGCGCGCC             GG^CGCGCC
AspHI         277  GAGCTC               G[AT]GC[AT]^C
AvaI          390  CTCGGG               C^[CT]CG[GA]G
BalI          257  TGGCCA               TGG^CCA
BanII         277  GAGCTC               G[GA]GC[CT]^C
BglI          406  GCCACCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          277  GAGCTC               G[AGT]GC[ACT]^C
BseRI         274  GAGGAG               GAGGAG(10/8)
BsiEI         305  CGATCG               CG[GA][CT]^CG
BsiHKAI       277  GAGCTC               G[AT]GC[AT]^C
BsoBI         390  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      277  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I       90  TGTACA               T^GTACA
BspMI         248  ACCTGC               ACCTGC(4/8)
BsrDI         203  GCAATG               GCAATG(2/0)
BsrGI          90  TGTACA               T^GTACA
BstYI         185  AGATCC               [GA]^GATC[CT]
DdeI          182  CTGAG                C^T[ACGT]AG
EaeI          257  TGGCCA               [CT]^GGCC[GA]
EcoT14I        38  CCAAGG               C^C[AT][AT]GG
HinfI         233  GAATC                G^A[ACGT]TC
HphI          176  TCACC                GGTGA(8/7)
KspI            9  CCGCGG               CCGC^GG
MaeIII        301  GTGAC                ^GT[ACGT]AC
MflI          185  AGATCC               [GA]^GATC[CT]
MluNI         257  TGGCCA               TGG^CCA
MscI          257  TGGCCA               TGG^CCA
PstI          250  CTGCAG               CTGCA^G
PvuI          305  CGATCG               CGAT^CG
PvuII         253  CAGCTG               CAG^CTG
SacI          277  GAGCTC               GAGCT^C
SacII           9  CCGCGG               CCGC^GG
SfcI          250  CTGCAG               C^T[GA][CT]AG
SfiI          405  GGCCACCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         135  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI          90  TGTACA               T^GTACA
StuI          413  AGGCCT               AGG^CCT
StyI           38  CCAAGG               C^C[AT][AT]GG
TfiI          233  GAATC                G^A[AT]TC
Tsp45I        301  GTGAC                ^GT[GC]AC
XcmI          362  CCAGCGCCACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         185  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BanI          133  GGCGCC               G^G[CT][GA]CC
              384  GGCGCC              
BbeI          133  GGCGCC               GGCGC^C
              384  GGCGCC              
BbsI          225  GAAGAC               GAAGAC(2/6)
              357  GAAGAC              
BpuAI         225  GAAGAC               GAAGAC(2/6)
              357  GAAGAC              
BssHII         60  GCGCGC               G^CGCGC
              140  GCGCGC              
DpnII         186  GATC                 ^GATC
              306  GATC                
DsaI            9  CCGCGG               C^C[GA][CT]GG
              371  CCGTGG              
HgaI          228  GACGC                GACGC(5/10)
              320  GCGTC               
KasI          133  GGCGCC               G^GCGCC
              384  GGCGCC              
MboI          186  GATC                 ^GATC
              306  GATC                
MboII         225  GAAGA                GAAGA(8/7)
              357  GAAGA               
NaeI           64  GCCGGC               GCC^GGC
              136  GCCGGC              
NarI          133  GGCGCC               GG^CGCC
              384  GGCGCC              
NdeII         186  GATC                 ^GATC
              306  GATC                
NgoMIV         64  GCCGGC               G^CCGGC
              136  GCCGGC              
Sau3AI        186  GATC                 ^GATC
              306  GATC                
SfoI          133  GGCGCC               GGC^GCC
              384  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
BbvI        BpmI        BsaHI       BsaJI       BsiYI       
BslI        BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       ItaI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NlaIV       
RsaI        Sau96I      ScrFI       TseI        TspRI       
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