Restriction map for pF1KB8737
 (Circular) (Six-base) MAPSORT of: pF1KB8737.seq  Check: 1038  from: 1  to: 3542



pF1KB8737  3542 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    365     365 
   Size:       3542
 
AccI GT'mk_AC

Cuts at:    580     580 
   Size:       3542
 
Acc65I G'GTAC_C

Cuts at:    558     558 
   Size:       3542
 
AclI AA'CG_TT

Cuts at:   3382    3382 
   Size:       3542
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1367    1799    2546    2814    1367 
   Size:        432     747     268    2095

  Fragments arranged by size: 

                2095     747     432     268
 
AflIII A'CryG_T

Cuts at:    894    1998    2975     894 
   Size:       1104     977    1461

  Fragments arranged by size: 

                1461    1104     977
 
AgeI A'CCGG_T

Cuts at:   1930    1930 
   Size:       3542
 
AlwNI CAG_nnn'CTG

Cuts at:    847    2414     847 
   Size:       1567    1975
 
ApaLI G'TGCA_C

Cuts at:   2312    2312 
   Size:       3542
 
ApoI r'AATT_y

Cuts at:    546    2895    3151     546 
   Size:       2349     256     937

  Fragments arranged by size: 

                2349     937     256
 
AscI GG'CGCG_CC

Cuts at:    248     248 
   Size:       3542
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3542
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3542
 
AvaI C'yCGr_G

Cuts at:    498     562     498 
   Size:         64    3478
 
BamHI G'GATC_C

Cuts at:    567     567 
   Size:       3542
 
BanI G'GyrC_C

Cuts at:    241     492     558     972    1220    1255     241 
   Size:        251      66     414     248      35    2528

  Fragments arranged by size: 

                2528     414     251     248      66      35
 
BanII G_rGCy'C

Cuts at:    389     556    1586     389 
   Size:        167    1030    2345

  Fragments arranged by size: 

                2345    1030     167
 
BbeI G_GCGC'C

Cuts at:    245     496     976    1224     245 
   Size:        251     480     248    2563

  Fragments arranged by size: 

                2563     480     251     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    340     472     340 
   Size:        132    3410
 
BciVI GTATCCnnnnn_n'

Cuts at:   1440    2201    3542    1440 
   Size:        761    1341    1440

  Fragments arranged by size: 

                1440    1341     761
 
BclI T'GATC_A

Cuts at:   1062    1062 
   Size:       3542
 
BglI GCCn_nnn'nGGC

Cuts at:    520     520 
   Size:       3542
 
BglII A'GATC_T

Cuts at:   1057    3094    1057 
   Size:       2037    1505
 
BlpI GC'TnA_GC

Cuts at:    654     654 
   Size:       3542
 
Bme1580I G_kGCm'C

Cuts at:   1167    1260    2316    1167 
   Size:         93    1056    2393

  Fragments arranged by size: 

                2393    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    181     754     835    1170    3314     181 
   Size:        573      81     335    2144     409

  Fragments arranged by size: 

                2144     573     409     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1909    1909 
   Size:       3542
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    163     295     319     163 
   Size:        132      24    3386

  Fragments arranged by size: 

                3386     132      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    718    1597    2089    2387     718 
   Size:        879     492     298    1873

  Fragments arranged by size: 

                1873     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3246    3246 
   Size:       3542
 
BsaAI yAC'GTr

Cuts at:    895     895 
   Size:       3542
 
BsaBI GATnn'nnATC

Cuts at:      6    1056    3093       6 
   Size:       1050    2037     455

  Fragments arranged by size: 

                2037    1050     455
 
BsaHI Gr'CG_yC

Cuts at:    242     428     493     973    1221    1923    3419     242 
   Size:        186      65     480     248     702    1496     365

  Fragments arranged by size: 

                1496     702     480     365     248     186      65
 
BsaWI w'CCGG_w

Cuts at:    731    1252    1930    2204    2351    2762     731 
   Size:        521     678     274     147     411    1511

  Fragments arranged by size: 

                1511     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    397     397 
   Size:       3542
 
BseYI C'CCAG_C

Cuts at:    399     967    1725    2302    2798     399 
   Size:        568     758     577     496    1143

  Fragments arranged by size: 

                1143     758     577     568     496
 
BsiEI CG_ry'CG

Cuts at:    104     416    1084    1130    2338     104 
   Size:        312     668      46    1208    1308

  Fragments arranged by size: 

                1308    1208     668     312      46
 
BsiHKAI G_wGCw'C

Cuts at:    389     556    1334    1524    2316     389 
   Size:        167     778     190     792    1615

  Fragments arranged by size: 

                1615     792     778     190     167
 
Bsp1286I G_dGCh'C

Cuts at:    389     556    1167    1260    1334    1524    1586    2316     389 
   Size:        167     611      93      74     190      62     730    1615

  Fragments arranged by size: 

                1615     730     611     190     167      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2762    2762 
   Size:       3542
 
BspHI T'CATG_A

Cuts at:   2718    2718 
   Size:       3542
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    365     592     806    1108    1489     365 
   Size:        227     214     302     381    2418

  Fragments arranged by size: 

                2418     381     302     227     214
 
BsrBI CCG'CTC

Cuts at:     50    1834    1888    3372    3537      50 
   Size:       1784      54    1484     165      55

  Fragments arranged by size: 

                1784    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    318    1454     318 
   Size:       1136    2406
 
BsrFI r'CCGG_y

Cuts at:    172     203     244    1540    1721    1930     172 
   Size:         31      41    1296     181     209    1784

  Fragments arranged by size: 

                1784    1296     209     181      41      31
 
BsrGI T'GTAC_A

Cuts at:    198     198 
   Size:       3542
 
BssHII G'CGCG_C

Cuts at:    168     248     168 
   Size:         80    3462
 
BssSI C'ACGA_G

Cuts at:   1813    2171    1813 
   Size:        358    3184
 
BstAPI GCAn_nnn'nTGC

Cuts at:    919     919 
   Size:       3542
 
BstBI TT'CG_AA

Cuts at:   1903    1903 
   Size:       3542
 
BstYI r'GATC_y

Cuts at:      1     293     567    1034    1057    1392    1638    2650    2736 
   Size:        292     274     467      23     335     246    1012      86

Cuts at:   2736    2748    3094       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     292     274     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    117     479     117 
   Size:        362    3180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1492    1673    1836    1492 
   Size:        181     163    3198

  Fragments arranged by size: 

                3198     181     163
 
BtsI GCAGTG_nn'

Cuts at:    275     785     275 
   Size:        510    3032
 
DraI TTT'AAA

Cuts at:    541     541 
   Size:       3542
 
DrdI GACnn_nn'nnGTC

Cuts at:   1248    2106    1248 
   Size:        858    2684
 
EaeI y'GGCC_r

Cuts at:    365    1127    1301    1692    1719    3064     365 
   Size:        762     174     391      27    1345     843

  Fragments arranged by size: 

                1345     843     762     391     174      27
 
EagI C'GGCC_G

Cuts at:   1127    1127 
   Size:       3542
 
EarI CTCTTCn'nnn_

Cuts at:   1565    1775    1565 
   Size:        210    3332
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1750    2060    2206    3352    1750 
   Size:        310     146    1146    1940

  Fragments arranged by size: 

                1940    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    387     554     387 
   Size:        167    3375
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    163     295     319    1367    1799    2546    2814     163 
   Size:        132      24    1048     432     747     268     891

  Fragments arranged by size: 

                1048     891     747     432     268     132      24
 
EcoO109I rG'GnC_Cy

Cuts at:    681     681 
   Size:       3542
 
EcoRI G'AATT_C

Cuts at:    546     546 
   Size:       3542
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1561    1561 
   Size:       3542
 
FspI TGC'GCA

Cuts at:   1323    1323 
   Size:       3542
 
HaeII r_GCGC'y

Cuts at:    245     476     496     976    1224    2246    3225     245 
   Size:        231      20     480     248    1022     979     562

  Fragments arranged by size: 

                1022     979     562     480     248     231      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1384    1414    1416    1446    1384 
   Size:         30       2      30    3480

  Fragments arranged by size: 

                3480      30      30       2
 
HincII GTy'rAC

Cuts at:    581     581 
   Size:       3542
 
KasI G'GCGC_C

Cuts at:    241     492     972    1220     241 
   Size:        251     480     248    2563

  Fragments arranged by size: 

                2563     480     251     248
 
KpnI G_GTAC'C

Cuts at:    562     562 
   Size:       3542
 
MluI A'CGCG_T

Cuts at:   2975    2975 
   Size:       3542
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2213    2397    2213 
   Size:        184    3358
 
MscI TGG'CCA

Cuts at:    367    1303     367 
   Size:        936    2606
 
MslI CAynn'nnrTG

Cuts at:   1531    1658    1531 
   Size:        127    3415
 
MspA1I CmG'CkG

Cuts at:    119     289     363     653     967    1327    2340    2585     119 
   Size:        170      74     290     314     360    1013     245    1076

  Fragments arranged by size: 

                1076    1013     360     314     290     245     170      74
            
 
NaeI GCC'GGC

Cuts at:    174     246    1723     174 
   Size:         72    1477    1993

  Fragments arranged by size: 

                1993    1477      72
 
NarI GG'CG_CC

Cuts at:    242     493     973    1221     242 
   Size:        251     480     248    2563

  Fragments arranged by size: 

                2563     480     251     248
 
NgoMIV G'CCGG_C

Cuts at:    172     244    1721     172 
   Size:         72    1477    1993

  Fragments arranged by size: 

                1993    1477      72
 
NspI r_CATG'y

Cuts at:    595    1095    2002     595 
   Size:        500     907    2135

  Fragments arranged by size: 

                2135     907     500
 
PasI CC'CwG_GG

Cuts at:    420     420 
   Size:       3542
 
PciI A'CATG_T

Cuts at:   1998    1998 
   Size:       3542
 
PfoI T'CCnGG_A

Cuts at:    296     296 
   Size:       3542
 
PmeI GTTT'AAAC

Cuts at:    541     541 
   Size:       3542
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2681    2713    2681 
   Size:         32    3510
 
PstI C_TGCA'G

Cuts at:    362     589    1274     362 
   Size:        227     685    2630

  Fragments arranged by size: 

                2630     685     227
 
PvuI CG_AT'CG

Cuts at:    104     416     104 
   Size:        312    3230
 
PvuII CAG'CTG

Cuts at:    363     967    1327     363 
   Size:        604     360    2578

  Fragments arranged by size: 

                2578     604     360
 
RsrII CG'GwC_CG

Cuts at:   1737    1737 
   Size:       3542
 
SacI G_AGCT'C

Cuts at:    389     556     389 
   Size:        167    3375
 
SacII CC_GC'GG

Cuts at:    120     120 
   Size:       3542
 
SalI G'TCGA_C

Cuts at:    579     579 
   Size:       3542
 
SapI GCTCTTCn'nnn_

Cuts at:   1565    1775    1565 
   Size:        210    3332
 
SbfI CC_TGCA'GG

Cuts at:    589     589 
   Size:       3542
 
ScaI AGT'ACT

Cuts at:   3059    3059 
   Size:       3542
 
SfcI C'TryA_G

Cuts at:     33     358     585    1270    2263    2454      33 
   Size:        325     227     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     325     227     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    520     520 
   Size:       3542
 
SfoI GGC'GCC

Cuts at:    243     494     974    1222     243 
   Size:        251     480     248    2563

  Fragments arranged by size: 

                2563     480     251     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3542
 
SgrAI Cr'CCGG_yG

Cuts at:    244     244 
   Size:       3542
 
SmaI CCC'GGG

Cuts at:    564     564 
   Size:       3542
 
SmlI C'TyrA_G

Cuts at:    697    1612    2104    2366     697 
   Size:        915     492     262    1873

  Fragments arranged by size: 

                1873     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3542
 
SphI G_CATG'C

Cuts at:    595    1095     595 
   Size:        500    3042
 
StuI AGG'CCT

Cuts at:    523     523 
   Size:       3542
 
StyI C'CwwG_G

Cuts at:    146     676    1030     146 
   Size:        530     354    2658

  Fragments arranged by size: 

                2658     530     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    532    1125    1258    1717    1926     532 
   Size:        593     133     459     209    2148

  Fragments arranged by size: 

                2148     593     459     209     133
 
TatI w'GTAC_w

Cuts at:    198    3057     198 
   Size:       2859     683
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3059    3091    3059 
   Size:         32    3510
 
Tth111I GACn'n_nGTC

Cuts at:   1339    1339 
   Size:       3542
 
XbaI T'CTAG_A

Cuts at:    573     573 
   Size:       3542
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    477    1037     477 
   Size:        560    2982
 
XmaI C'CCGG_G

Cuts at:    562     562 
   Size:       3542
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI     PciI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsaXI     BsgI    BsiWI     BsmI    BsmBI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SanDI    SexAI    SnaBI     SrfI     SspI     SwaI
     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com