Restriction map for pF1KB8737
 (Linear) (Six-base) MAPSORT of: KIBB8737.seq  Check: 34  from: 1  to: 429



KIBB8737.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     257     429 
   Size:        257     172
 
AscI GG'CGCG_CC

Cuts at:      0     140     429 
   Size:        140     289
 
AvaI C'yCGr_G

Cuts at:      0     390     429 
   Size:        390      39
 
BanI G'GyrC_C

Cuts at:      0     133     384     429 
   Size:        133     251      45

  Fragments arranged by size: 

                 251     133      45
 
BanII G_rGCy'C

Cuts at:      0     281     429 
   Size:        281     148
 
BbeI G_GCGC'C

Cuts at:      0     137     388     429 
   Size:        137     251      41

  Fragments arranged by size: 

                 251     137      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     364     429 
   Size:        232     132      65

  Fragments arranged by size: 

                 232     132      65
 
BglI GCCn_nnn'nGGC

Cuts at:      0     412     429 
   Size:        412      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      73     429 
   Size:         73     356
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     187     211     429 
   Size:         55     132      24     218

  Fragments arranged by size: 

                 218     132      55      24
 
BsaHI Gr'CG_yC

Cuts at:      0     134     320     385     429 
   Size:        134     186      65      44

  Fragments arranged by size: 

                 186     134      65      44
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     429 
   Size:        289     140
 
BseYI C'CCAG_C

Cuts at:      0     291     429 
   Size:        291     138
 
BsiEI CG_ry'CG

Cuts at:      0     308     429 
   Size:        308     121
 
BsiHKAI G_wGCw'C

Cuts at:      0     281     429 
   Size:        281     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     281     429 
   Size:        281     148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     257     429 
   Size:        257     172
 
BsrDI GCAATG_nn'

Cuts at:      0     210     429 
   Size:        210     219
 
BsrFI r'CCGG_y

Cuts at:      0      64      95     136     429 
   Size:         64      31      41     293

  Fragments arranged by size: 

                 293      64      41      31
 
BsrGI T'GTAC_A

Cuts at:      0      90     429 
   Size:         90     339
 
BssHII G'CGCG_C

Cuts at:      0      60     140     429 
   Size:         60      80     289

  Fragments arranged by size: 

                 289      80      60
 
BstYI r'GATC_y

Cuts at:      0     185     429 
   Size:        185     244
 
BtgI C'CryG_G

Cuts at:      0       9     371     429 
   Size:          9     362      58

  Fragments arranged by size: 

                 362      58       9
 
BtsI GCAGTG_nn'

Cuts at:      0     167     429 
   Size:        167     262
 
EaeI y'GGCC_r

Cuts at:      0     257     429 
   Size:        257     172
 
EcoICRI GAG'CTC

Cuts at:      0     279     429 
   Size:        279     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     187     211     429 
   Size:         55     132      24     218

  Fragments arranged by size: 

                 218     132      55      24
 
HaeII r_GCGC'y

Cuts at:      0     137     368     388     429 
   Size:        137     231      20      41

  Fragments arranged by size: 

                 231     137      41      20
 
KasI G'GCGC_C

Cuts at:      0     133     384     429 
   Size:        133     251      45

  Fragments arranged by size: 

                 251     133      45
 
MscI TGG'CCA

Cuts at:      0     259     429 
   Size:        259     170
 
MspA1I CmG'CkG

Cuts at:      0      11     181     255     429 
   Size:         11     170      74     174

  Fragments arranged by size: 

                 174     170      74      11
 
NaeI GCC'GGC

Cuts at:      0      66     138     429 
   Size:         66      72     291

  Fragments arranged by size: 

                 291      72      66
 
NarI GG'CG_CC

Cuts at:      0     134     385     429 
   Size:        134     251      44

  Fragments arranged by size: 

                 251     134      44
 
NgoMIV G'CCGG_C

Cuts at:      0      64     136     429 
   Size:         64      72     293

  Fragments arranged by size: 

                 293      72      64
 
PasI CC'CwG_GG

Cuts at:      0     312     429 
   Size:        312     117
 
PfoI T'CCnGG_A

Cuts at:      0     188     429 
   Size:        188     241
 
PstI C_TGCA'G

Cuts at:      0     254     429 
   Size:        254     175
 
PvuI CG_AT'CG

Cuts at:      0     308     429 
   Size:        308     121
 
PvuII CAG'CTG

Cuts at:      0     255     429 
   Size:        255     174
 
SacI G_AGCT'C

Cuts at:      0     281     429 
   Size:        281     148
 
SacII CC_GC'GG

Cuts at:      0      12     429 
   Size:         12     417
 
SfcI C'TryA_G

Cuts at:      0     250     429 
   Size:        250     179
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     412     429 
   Size:        412      17
 
SfoI GGC'GCC

Cuts at:      0     135     386     429 
   Size:        135     251      43

  Fragments arranged by size: 

                 251     135      43
 
SgrAI Cr'CCGG_yG

Cuts at:      0     136     429 
   Size:        136     293
 
StuI AGG'CCT

Cuts at:      0     415     429 
   Size:        415      14
 
StyI C'CwwG_G

Cuts at:      0      38     429 
   Size:         38     391
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     424     429 
   Size:        424       5
 
TatI w'GTAC_w

Cuts at:      0      90     429 
   Size:         90     339
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     369     429 
   Size:        369      60
 

 Enzymes that do cut:

     AarI     AscI     AvaI     BanI    BanII     BbeI     BbsI     BglI
     BmrI     BpmI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BstYI     BtgI     BtsI
     EaeI  EcoICRI  Eco57MI    HaeII     KasI     MscI   MspA1I     NaeI
     NarI   NgoMIV     PasI     PfoI     PstI     PvuI    PvuII     SacI
    SacII     SfcI     SfiI     SfoI    SgrAI     StuI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com