Restriction map for pF1KB8736
 (Linear) (Six-base) MAPSORT of: KIBB8736.seq  Check: 6429  from: 1  to: 1200



KIBB8736.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     789     957    1041    1200 
   Size:         84     705     168      84     159

  Fragments arranged by size: 

                 705     168     159      84      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0     707    1200 
   Size:        707     493
 
ApoI r'AATT_y

Cuts at:      0      25     263     550    1200 
   Size:         25     238     287     650

  Fragments arranged by size: 

                 650     287     238      25
 
BanI G'GyrC_C

Cuts at:      0     659    1200 
   Size:        659     541
 
BanII G_rGCy'C

Cuts at:      0     176     210     985    1200 
   Size:        176      34     775     215

  Fragments arranged by size: 

                 775     215     176      34
 
BbeI G_GCGC'C

Cuts at:      0     663    1200 
   Size:        663     537
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     141    1200 
   Size:        141    1059
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     836    1088    1200 
   Size:        836     252     112

  Fragments arranged by size: 

                 836     252     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     701     785     869     953    1037    1121    1200 
   Size:        112     589      84      84      84      84      84      79

  Fragments arranged by size: 

                 589     112      84      84      84      84      84      79
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     439     477    1200 
   Size:        439      38     723

  Fragments arranged by size: 

                 723     439      38
 
BsaHI Gr'CG_yC

Cuts at:      0     660    1200 
   Size:        660     540
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     814    1200 
   Size:        814     386
 
BsiHKAI G_wGCw'C

Cuts at:      0     985    1200 
   Size:        985     215
 
BsmI GAATG_Cn'

Cuts at:      0     512     703     787     955    1200 
   Size:        512     191      84     168     245

  Fragments arranged by size: 

                 512     245     191     168      84
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     824    1200 
   Size:        824     376
 
Bsp1286I G_dGCh'C

Cuts at:      0     176     210     985    1200 
   Size:        176      34     775     215

  Fragments arranged by size: 

                 775     215     176      34
 
BstYI r'GATC_y

Cuts at:      0     529    1200 
   Size:        529     671
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     684    1200 
   Size:        684     516
 
BtsI GCAGTG_nn'

Cuts at:      0     708     792    1200 
   Size:        708      84     408

  Fragments arranged by size: 

                 708     408      84
 
EaeI y'GGCC_r

Cuts at:      0     110     360    1200 
   Size:        110     250     840

  Fragments arranged by size: 

                 840     250     110
 
EarI CTCTTCn'nnn_

Cuts at:      0     217     708    1200 
   Size:        217     491     492

  Fragments arranged by size: 

                 492     491     217
 
EcoICRI GAG'CTC

Cuts at:      0     983    1200 
   Size:        983     217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     112     701     785     789     869     953     957 
   Size:         84      28     589      84       4      80      84       4

Cuts at:    957    1037    1041    1121    1200 
   Size:         80       4      80      79

  Fragments arranged by size: 

                 589      84      84      84      80      80      80      79
                  28       4       4       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69    1136    1200 
   Size:         69    1067      64

  Fragments arranged by size: 

                1067      69      64
 
EcoRI G'AATT_C

Cuts at:      0      25     550    1200 
   Size:         25     525     650

  Fragments arranged by size: 

                 650     525      25
 
HaeII r_GCGC'y

Cuts at:      0     663    1200 
   Size:        663     537
 
HincII GTy'rAC

Cuts at:      0     413     501    1200 
   Size:        413      88     699

  Fragments arranged by size: 

                 699     413      88
 
HindIII A'AGCT_T

Cuts at:      0     474    1200 
   Size:        474     726
 
HpaI GTT'AAC

Cuts at:      0     501    1200 
   Size:        501     699
 
KasI G'GCGC_C

Cuts at:      0     659    1200 
   Size:        659     541
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     137    1200 
   Size:        137    1063
 
MscI TGG'CCA

Cuts at:      0     112     362    1200 
   Size:        112     250     838

  Fragments arranged by size: 

                 838     250     112
 
MslI CAynn'nnrTG

Cuts at:      0     517    1200 
   Size:        517     683
 
MspA1I CmG'CkG

Cuts at:      0      51    1200 
   Size:         51    1149
 
NarI GG'CG_CC

Cuts at:      0     660    1200 
   Size:        660     540
 
NdeI CA'TA_TG

Cuts at:      0     141    1200 
   Size:        141    1059
 
PpuMI rG'GwC_Cy

Cuts at:      0      69    1200 
   Size:         69    1131
 
PsiI TTA'TAA

Cuts at:      0     452     536    1200 
   Size:        452      84     664

  Fragments arranged by size: 

                 664     452      84
 
PstI C_TGCA'G

Cuts at:      0     326    1200 
   Size:        326     874
 
PvuII CAG'CTG

Cuts at:      0      51    1200 
   Size:         51    1149
 
SacI G_AGCT'C

Cuts at:      0     985    1200 
   Size:        985     215
 
SapI GCTCTTCn'nnn_

Cuts at:      0     708    1200 
   Size:        708     492
 
SfcI C'TryA_G

Cuts at:      0      75     322     834    1200 
   Size:         75     247     512     366

  Fragments arranged by size: 

                 512     366     247      75
 
SfoI GGC'GCC

Cuts at:      0     661    1200 
   Size:        661     539
 
StyI C'CwwG_G

Cuts at:      0     239    1200 
   Size:        239     961
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     608    1200 
   Size:        608     592
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     886     918    1200 
   Size:        886      32     282

  Fragments arranged by size: 

                 886     282      32
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI    BanII     BbeI    BciVI     BplI
     BpmI   Bpu10I    BsaHI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BstYI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    HaeII   HincII  HindIII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NdeI    PpuMI     PsiI     PstI    PvuII
     SacI     SapI     SfcI     SfoI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I     KpnI     MfeI     MluI     NaeI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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