Restriction map for pF1KB8718
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AgeI        
AhdI        AlwNI       Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BbrPI       BbsI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaI        BsiWI       BsmI        
Bsp1407I    BspDI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MfeI        MluI        MseI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          449  GTCTAC               GT^[AC][GT]AC
Alw44I        672  GTGCAC               G^TGCAC
ApaLI         672  GTGCAC               G^TGCAC
Asp700I       936  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1594  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1810  GGATCC               G^GATCC
BanII          34  GAGCTC               G[GA]GC[CT]^C
BbeI          394  GGCGCC               GGCGC^C
BbvCI        1711  GCTGAGG              CCTCAGC(-5/-2)
BfaI          679  CTAG                 C^TAG
BlpI          711  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      711  GCTCAGC              GC^T[ACGT]AGC
BsaBI         964  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1093  GAGACG               CGTCTC(1/5)
BspHI        1139  TCATGA               T^CATGA
BspMI         604  GCAGGT               ACCTGC(4/8)
BsrDI         125  GCAATG               GCAATG(2/0)
CelII         711  GCTCAGC              GC^T[ACGT]AGC
DraIII       1066  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1236  GAAGAG               CTCTTC(1/4)
EcoNI        1100  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1351  GTCAAC               GT[CT]^[GA]AC
HindII       1351  GTCAAC               GT[CT]^[GA]AC
KasI          394  GGCGCC               G^GCGCC
Ksp632I      1236  GAAGAG               CTCTTC(1/4)
MaeI          679  CTAG                 C^TAG
MamI          964  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1018  GCCGGC               GCC^GGC
NarI          394  GGCGCC               GG^CGCC
NgoMIV       1018  GCCGGC               G^CCGGC
PaeR7I        232  CTCGAG               C^TCGAG
PflFI        1594  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          906  GACTC                GAGTC(4/5)
PshAI        1368  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          520  CTGCAG               CTGCA^G
RcaI         1139  TCATGA               T^CATGA
SacI           34  GAGCTC               GAGCT^C
SapI         1236  GAAGAGC              GCTCTTC(1/4)
SfoI          394  GGCGCC               GGC^GCC
SmaI          110  CCCGGG               CCC^GGG
TfiI         1330  GATTC                G^A[AT]TC
Tth111I      1594  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          232  CTCGAG               C^TCGAG
XmaCI         110  CCCGGG               C^CCGGG
XmaI          110  CCCGGG               C^CCGGG
XmnI          936  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AatII        1261  GACGTC               GACGT^C
             1597  GACGTC              
AccIII        950  TCCGGA               T^CCGGA
             1739  TCCGGA              
BcgI          998  GCAACGTGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1241  GCAGCAAGGTCG        
BseAI         950  TCCGGA               T^CCGGA
             1739  TCCGGA              
BsiEI         657  CGACCG               CG[GA][CT]^CG
              699  CGACCG              
BsmAI         631  GAGAC                GTCTC(1/5)
             1093  GAGAC               
BspEI         950  TCCGGA               T^CCGGA
             1739  TCCGGA              
BspLU11I     1496  ACATGT               A^CATGT
             1787  ACATGT              
BsrFI         683  ACCGGC               [GA]^CCGG[CT]
             1018  GCCGGC              
BstYI        1614  GGATCT               [GA]^GATC[CT]
             1810  GGATCC              
Cfr10I        683  ACCGGC               [GA]^CCGG[CT]
             1018  GCCGGC              
DrdI          658  GACCGGATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1182  GACGGAGGTGTC        
DsaI          366  CCGTGG               C^C[GA][CT]GG
             1670  CCGTGG              
HaeII         158  AGCGCC               [GA]GCGC^[CT]
              394  GGCGCC              
HgaI          954  GACGC                GACGC(5/10)
             1095  GACGC               
HinfI         906  GACTC                G^A[ACGT]TC
             1330  GATTC               
MaeIII        811  GTCAC                ^GT[ACGT]AC
             1405  GTCAC               
MflI         1614  GGATCT               [GA]^GATC[CT]
             1810  GGATCC              
MroI          950  TCCGGA               T^CCGGA
             1739  TCCGGA              
PflMI         306  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              559  CCAAGTGCTGG         
PpuMI         227  AGGACCT              [GA]G^G[AT]CC[CT]
              419  GGGACCT             
SfcI          514  CTACAG               C^T[GA][CT]AG
              520  CTGCAG              
SmlI           37  CTCAAG               C^T[CT][GA]AG
              232  CTCGAG              
Tsp45I        811  GTCAC                ^GT[GC]AC
             1405  GTCAC               
Van91I        306  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              559  CCAAGTGCTGG         
XhoII        1614  GGATCT               [GA]^GATC[CT]
             1810  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AocI        AspHI       AvaI        
AvaII       BalI        BanI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeII       MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        TspRI       TthHB8I     
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