Restriction map for
pF1KB8672
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FseI FspI HaeII HhaI HinP1I
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 187 GTCTAC GT^[AC][GT]AC
AcyI 1381 GGCGTC G[GA]^CG[CT]C
AflIII 1292 ACATGT A^C[GA][CT]GT
AspHI 154 GAGCTC G[AT]GC[AT]^C
BamHI 43 GGATCC G^GATCC
BbsI 493 GAAGAC GAAGAC(2/6)
BciVI 640 GTATCC GTATCC(6/5)
BclI 404 TGATCA T^GATCA
BcnI 1326 CCCGG CC^[GC]GG
BglI 562 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 865 GCTGAGC GC^T[ACGT]AGC
BpmI 778 CTGGAG CTGGAG(16/14)
Bpu1102I 865 GCTGAGC GC^T[ACGT]AGC
BpuAI 493 GAAGAC GAAGAC(2/6)
BsaHI 1381 GGCGTC G[GA]^CG[CT]C
BsaI 897 GGTCTC GGTCTC(1/5)
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1482 TGTACA T^GTACA
BspLU11I 1292 ACATGT A^CATGT
BsrGI 1482 TGTACA T^GTACA
BstAPI 418 GCAAATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 644 CCATGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 43 GGATCC [GA]^GATC[CT]
CelII 865 GCTGAGC GC^T[ACGT]AGC
EarI 721 GAAGAG CTCTTC(1/4)
EcoRV 1219 GATATC GAT^ATC
FauI 10 GCGGG CCCGC(4/6)
FbaI 404 TGATCA T^GATCA
HapII 1327 CCGG C^CGG
HgaI 1382 GCGTC GACGC(5/10)
Hin1I 1381 GGCGTC G[GA]^CG[CT]C
HincII 546 GTTAAC GT[CT]^[GA]AC
HindII 546 GTTAAC GT[CT]^[GA]AC
HindIII 1553 AAGCTT A^AGCTT
HpaI 546 GTTAAC GTT^AAC
HpaII 1327 CCGG C^CGG
Ksp632I 721 GAAGAG CTCTTC(1/4)
MflI 43 GGATCC [GA]^GATC[CT]
MspA1I 742 CAGCTG C[AC]G^C[GT]G
MspI 1327 CCGG C^CGG
NciI 1326 CCCGG CC^[GC]GG
NspI 1292 ACATGT [GA]CATG^[CT]
PshAI 180 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 742 CAGCTG CAG^CTG
SacI 154 GAGCTC GAGCT^C
ScaI 439 AGTACT AGT^ACT
SfcI 1070 CTGTAG C^T[GA][CT]AG
SspBI 1482 TGTACA T^GTACA
TaqI 1092 TCGA T^CGA
TthHB8I 1092 TCGA T^CGA
XhoII 43 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 44 GATCC GGATC(4/5)
43 GGATC
BalI 434 TGGCCA TGG^CCA
576 TGGCCA
BanII 154 GAGCTC G[GA]GC[CT]^C
868 GAGCCC
BbvI 1390 GCTGC GCAGC(8/12)
1437 GCTGC
BsmFI 1005 GGGAC GGGAC(10/14)
1324 GTCCC
BsmI 255 GAATGC GAATGC(1/-1)
519 GAATGC
BsrDI 1386 CATTGC GCAATG(2/0)
1594 GCAATG
DsaI 372 CCATGG C^C[GA][CT]GG
566 CCATGG
Fnu4HI 1390 GCTGC GC^[ACGT]GC
1437 GCTGC
HphI 238 GGTGA GGTGA(8/7)
980 TCACC
ItaI 1390 GCTGC GC^[ACGT]GC
1437 GCTGC
MaeII 659 ACGT A^CGT
1089 ACGT
MaeIII 239 GTGAC ^GT[ACGT]AC
1426 GTGAC
MluNI 434 TGGCCA TGG^CCA
576 TGGCCA
MscI 434 TGGCCA TGG^CCA
576 TGGCCA
NcoI 372 CCATGG C^CATGG
566 CCATGG
PleI 808 GACTC GAGTC(4/5)
1246 GAGTC
SspI 421 AATATT AAT^ATT
1318 AATATT
TseI 1390 GCTGC G^C[AT]GC
1437 GCTGC
Tsp45I 239 GTGAC ^GT[GC]AC
1426 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FokI HaeIII HinfI MaeI MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I Tsp509I