Restriction map for pF1KB8672
 (Circular) (Six-base) MAPSORT of: pF1KB8672.seq  Check: 5622  from: 1  to: 4826



pF1KB8672  4826 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    296    1864     296 
   Size:       1568    3258
 
Acc65I G'GTAC_C

Cuts at:   1842    1842 
   Size:       4826
 
AclI AA'CG_TT

Cuts at:   4666    4666 
   Size:       4826
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1679    2651    3083    3436    4098    1679 
   Size:        972     432     353     662    2407

  Fragments arranged by size: 

                2407     972     662     432     353
 
AflIII A'CryG_T

Cuts at:   1400    2178    3978    4259    1400 
   Size:        778    1800     281    1967

  Fragments arranged by size: 

                1967    1800     778     281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    733     765     733 
   Size:         32    4794
 
AlwNI CAG_nnn'CTG

Cuts at:    319    1116    1545    2131    3569     319 
   Size:        797     429     586    1438    1576

  Fragments arranged by size: 

                1576    1438     797     586     429
 
ApaLI G'TGCA_C

Cuts at:   3664    3664 
   Size:       4826
 
ApoI r'AATT_y

Cuts at:    181     537     855    1830    4179    4435     181 
   Size:        356     318     975    2349     256     572

  Fragments arranged by size: 

                2349     975     572     356     318     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4826
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4826
 
AvaI C'yCGr_G

Cuts at:   1846    1846 
   Size:       4826
 
BamHI G'GATC_C

Cuts at:    151    1851     151 
   Size:       1700    3126
 
BanI G'GyrC_C

Cuts at:   1842    2256    2504    2539    1842 
   Size:        414     248      35    4129

  Fragments arranged by size: 

                4129     414     248      35
 
BanII G_rGCy'C

Cuts at:    266     980    1840    2870     266 
   Size:        714     860    1030    2222

  Fragments arranged by size: 

                2222    1030     860     714
 
BbeI G_GCGC'C

Cuts at:   2260    2508    2260 
   Size:        248    4578
 
BbsI GAAGACnn'nnnn_

Cuts at:    608     608 
   Size:       4826
 
BciVI GTATCCnnnnn_n'

Cuts at:    759    2724    3780    4826     759 
   Size:       1965    1056    1046     759

  Fragments arranged by size: 

                1965    1056    1046     759
 
BclI T'GATC_A

Cuts at:    512    2346     512 
   Size:       1834    2992
 
BglI GCCn_nnn'nGGC

Cuts at:    676     676 
   Size:       4826
 
BglII A'GATC_T

Cuts at:   2341    4378    2341 
   Size:       2037    2789
 
BlpI GC'TnA_GC

Cuts at:    974    1938     974 
   Size:        964    3862
 
Bme1580I G_kGCm'C

Cuts at:   1460    2451    2544    3668    1460 
   Size:        991      93    1124    2618

  Fragments arranged by size: 

                2618    1124     991      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    360     703    2038    2119    2454    4598     360 
   Size:        343    1335      81     335    2144     588

  Fragments arranged by size: 

                2144    1335     588     343     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3193    3193 
   Size:       4826
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    907     907 
   Size:       4826
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2002    2881    3595    3893    2002 
   Size:        879     714     298    2935

  Fragments arranged by size: 

                2935     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1011    4530    1011 
   Size:       3519    1307
 
BsaAI yAC'GTr

Cuts at:   2179    2179 
   Size:       4826
 
BsaBI GATnn'nnATC

Cuts at:      6    2340    4377       6 
   Size:       2334    2037     455

  Fragments arranged by size: 

                2334    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1490    2257    2505    3207    4703    1490 
   Size:        767     248     702    1496    1613

  Fragments arranged by size: 

                1613    1496     767     702     248
 
BsaWI w'CCGG_w

Cuts at:   2015    2536    3214    3625    3772    4046    2015 
   Size:        521     678     411     147     274    2795

  Fragments arranged by size: 

                2795     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    798    2251    3009    3674    4082     798 
   Size:       1453     758     665     408    1542

  Fragments arranged by size: 

                1542    1453     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    2368    2414    3644     104 
   Size:       2264      46    1230    1286

  Fragments arranged by size: 

                2264    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    266    1840    2618    2808    3668     266 
   Size:       1574     778     190     860    1424

  Fragments arranged by size: 

                1574    1424     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    369     633     369 
   Size:        264    4562
 
Bsp1286I G_dGCh'C

Cuts at:    266     980    1460    1840    2451    2544    2618    2808    2870 
   Size:        714     480     380     611      93      74     190      62

Cuts at:   2870    3668     266 
   Size:        798    1424

  Fragments arranged by size: 

                1424     798     714     611     480     380     190      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   3258    3258 
   Size:       4826
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1876    2090    2392    2773    1876 
   Size:        214     302     381    3929

  Fragments arranged by size: 

                3929     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3118    3172    4656    4821      50 
   Size:       3068      54    1484     165      55

  Fragments arranged by size: 

                3068    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1493    1709    2738    1493 
   Size:        216    1029    3581

  Fragments arranged by size: 

                3581    1029     216
 
BsrFI r'CCGG_y

Cuts at:   2824    3005    2824 
   Size:        181    4645
 
BsrGI T'GTAC_A

Cuts at:   1590    1590 
   Size:       4826
 
BssSI C'ACGA_G

Cuts at:   3097    3805    3097 
   Size:        708    4118
 
BstAPI GCAn_nnn'nTGC

Cuts at:    532    2203     532 
   Size:       1671    3155
 
BstBI TT'CG_AA

Cuts at:   3187    3187 
   Size:       4826
 
BstXI CCAn_nnnn'nTGG

Cuts at:    759     759 
   Size:       4826
 
BstYI r'GATC_y

Cuts at:      1     151    1851    2318    2341    2676    2922    3228    3240 
   Size:        150    1700     467      23     335     246     306      12

Cuts at:   3240    3326    4378       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1700    1052     467     449     335     306     246     150
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    480     674     480 
   Size:        194    4632
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2776    2957    3120    2776 
   Size:        181     163    4482

  Fragments arranged by size: 

                4482     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2069    2069 
   Size:       4826
 
DraI TTT'AAA

Cuts at:   1825    1825 
   Size:       4826
 
DrdI GACnn_nn'nnGTC

Cuts at:   2532    3876    2532 
   Size:       1344    3482
 
EaeI y'GGCC_r

Cuts at:    158     542     684    2411    2585    2976    3003    4348     158 
   Size:        384     142    1727     174     391      27    1345     636

  Fragments arranged by size: 

                1727    1345     636     391     384     174     142      27
            
 
EagI C'GGCC_G

Cuts at:   2411    2411 
   Size:       4826
 
EarI CTCTTCn'nnn_

Cuts at:    824    2849    3059     824 
   Size:       2025     210    2591

  Fragments arranged by size: 

                2591    2025     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3034    3776    3922    4636    3034 
   Size:        742     146     714    3224

  Fragments arranged by size: 

                3224     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    264    1838     264 
   Size:       1574    3252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    907    1679    2651    3083    3436    4098     907 
   Size:        772     972     432     353     662    1635

  Fragments arranged by size: 

                1635     972     772     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:   1965    1965 
   Size:       4826
 
EcoRI G'AATT_C

Cuts at:   1830    1830 
   Size:       4826
 
EcoRV GAT'ATC

Cuts at:   1329    1329 
   Size:       4826
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    838    2845     838 
   Size:       2007    2819
 
FspI TGC'GCA

Cuts at:   2607    2607 
   Size:       4826
 
HaeII r_GCGC'y

Cuts at:   2260    2508    3738    4509    2260 
   Size:        248    1230     771    2577

  Fragments arranged by size: 

                2577    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    326     358     476     508    1318    1350    2668    2698    2700 
   Size:         32     118      32     810      32    1318      30       2

Cuts at:   2700    2730     326 
   Size:         30    2422

  Fragments arranged by size: 

                2422    1318     810     118      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    656    1865     656 
   Size:       1209    3617
 
HindIII A'AGCT_T

Cuts at:   1661    1661 
   Size:       4826
 
HpaI GTT'AAC

Cuts at:    656     656 
   Size:       4826
 
KasI G'GCGC_C

Cuts at:   2256    2504    2256 
   Size:        248    4578
 
KpnI G_GTAC'C

Cuts at:   1846    1846 
   Size:       4826
 
MluI A'CGCG_T

Cuts at:   4259    4259 
   Size:       4826
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3585    3769    3585 
   Size:        184    4642
 
MscI TGG'CCA

Cuts at:    544     686    2587     544 
   Size:        142    1901    2783

  Fragments arranged by size: 

                2783    1901     142
 
MslI CAynn'nnrTG

Cuts at:    436     458     757    2815    2942     436 
   Size:         22     299    2058     127    2320

  Fragments arranged by size: 

                2320    2058     299     127      22
 
MspA1I CmG'CkG

Cuts at:    852    1937    2251    2611    3395    3640     852 
   Size:       1085     314     360     784     245    2038

  Fragments arranged by size: 

                2038    1085     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3007    3007 
   Size:       4826
 
NarI GG'CG_CC

Cuts at:   2257    2505    2257 
   Size:        248    4578
 
NcoI C'CATG_G

Cuts at:    480     674     480 
   Size:        194    4632
 
NgoMIV G'CCGG_C

Cuts at:   3005    3005 
   Size:       4826
 
NspI r_CATG'y

Cuts at:   1404    1879    2379    3982    1404 
   Size:        475     500    1603    2248

  Fragments arranged by size: 

                2248    1603     500     475
 
PciI A'CATG_T

Cuts at:   1400    3978    1400 
   Size:       2578    2248
 
PfoI T'CCnGG_A

Cuts at:   1433    1433 
   Size:       4826
 
PmeI GTTT'AAAC

Cuts at:   1825    1825 
   Size:       4826
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3272    3304    3272 
   Size:         32    4794
 
PshAI GACnn'nnGTC

Cuts at:    292     292 
   Size:       4826
 
PsiI TTA'TAA

Cuts at:   1582    1582 
   Size:       4826
 
PstI C_TGCA'G

Cuts at:   1873    2558    1873 
   Size:        685    4141
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4826
 
PvuII CAG'CTG

Cuts at:    852    2251    2611     852 
   Size:       1399     360    3067

  Fragments arranged by size: 

                3067    1399     360
 
RsrII CG'GwC_CG

Cuts at:   3021    3021 
   Size:       4826
 
SacI G_AGCT'C

Cuts at:    266    1840     266 
   Size:       1574    3252
 
SalI G'TCGA_C

Cuts at:   1863    1863 
   Size:       4826
 
SapI GCTCTTCn'nnn_

Cuts at:   2849    3059    2849 
   Size:        210    4616
 
SbfI CC_TGCA'GG

Cuts at:   1873    1873 
   Size:       4826
 
ScaI AGT'ACT

Cuts at:    549    4343     549 
   Size:       3794    1032
 
SfcI C'TryA_G

Cuts at:     33    1178    1869    2554    3522    3713      33 
   Size:       1145     691     685     968     191    1146

  Fragments arranged by size: 

                1146    1145     968     691     685     191
 
SfoI GGC'GCC

Cuts at:   2258    2506    2258 
   Size:        248    4578
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4826
 
SmaI CCC'GGG

Cuts at:   1848    1848 
   Size:       4826
 
SmlI C'TyrA_G

Cuts at:   1981    2896    3610    3872    1981 
   Size:        915     714     262    2935

  Fragments arranged by size: 

                2935     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4826
 
SphI G_CATG'C

Cuts at:   1879    2379    1879 
   Size:        500    4326
 
SspI AAT'ATT

Cuts at:    531    1428     531 
   Size:        897    3929
 
StyI C'CwwG_G

Cuts at:    155     195     480     674    1960    2314     155 
   Size:         40     285     194    1286     354    2667

  Fragments arranged by size: 

                2667    1286     354     285     194      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    342     895    1498    2409    2542    3001    3210     342 
   Size:        553     603     911     133     459     209    1958

  Fragments arranged by size: 

                1958     911     603     553     459     209     133
 
TatI w'GTAC_w

Cuts at:    547    1590    4341     547 
   Size:       1043    2751    1032

  Fragments arranged by size: 

                2751    1043    1032
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4343    4375    4343 
   Size:         32    4794
 
Tth111I GACn'n_nGTC

Cuts at:   2623    2623 
   Size:       4826
 
XbaI T'CTAG_A

Cuts at:   1857    1857 
   Size:       4826
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2321    2321 
   Size:       4826
 
XmaI C'CCGG_G

Cuts at:   1846    1846 
   Size:       4826
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AloI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BspEI
   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
     FseI    FspAI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     XmnI     ZraI
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