Restriction map for pF1KB8672
 (Linear) (Six-base) MAPSORT of: KIBB8672.seq  Check: 4591  from: 1  to: 1713



KIBB8672.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     188    1713 
   Size:        188    1525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1571    1713 
   Size:       1571     142
 
AflIII A'CryG_T

Cuts at:      0    1292    1713 
   Size:       1292     421
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     625     657    1713 
   Size:        625      32    1056

  Fragments arranged by size: 

                1056     625      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     211    1008    1437    1713 
   Size:        211     797     429     276

  Fragments arranged by size: 

                 797     429     276     211
 
ApoI r'AATT_y

Cuts at:      0      73     429     747    1713 
   Size:         73     356     318     966

  Fragments arranged by size: 

                 966     356     318      73
 
BamHI G'GATC_C

Cuts at:      0      43    1713 
   Size:         43    1670
 
BanII G_rGCy'C

Cuts at:      0     158     872    1713 
   Size:        158     714     841

  Fragments arranged by size: 

                 841     714     158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     500    1713 
   Size:        500    1213
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     651    1713 
   Size:        651    1062
 
BclI T'GATC_A

Cuts at:      0     404    1713 
   Size:        404    1309
 
BglI GCCn_nnn'nGGC

Cuts at:      0     568    1713 
   Size:        568    1145
 
BlpI GC'TnA_GC

Cuts at:      0     866    1713 
   Size:        866     847
 
Bme1580I G_kGCm'C

Cuts at:      0    1352    1713 
   Size:       1352     361
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     252     595    1713 
   Size:        252     343    1118

  Fragments arranged by size: 

                1118     343     252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1713 
   Size:        799     914
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     903    1713 
   Size:        903     810
 
BsaHI Gr'CG_yC

Cuts at:      0    1382    1713 
   Size:       1382     331
 
BseYI C'CCAG_C

Cuts at:      0     690    1713 
   Size:        690    1023
 
BsiHKAI G_wGCw'C

Cuts at:      0     158    1713 
   Size:        158    1555
 
BsmI GAATG_Cn'

Cuts at:      0     261     525    1713 
   Size:        261     264    1188

  Fragments arranged by size: 

                1188     264     261
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     872    1352    1713 
   Size:        158     714     480     361

  Fragments arranged by size: 

                 714     480     361     158
 
BsrDI GCAATG_nn'

Cuts at:      0    1385    1601    1713 
   Size:       1385     216     112

  Fragments arranged by size: 

                1385     216     112
 
BsrGI T'GTAC_A

Cuts at:      0    1482    1713 
   Size:       1482     231
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     424    1713 
   Size:        424    1289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     651    1713 
   Size:        651    1062
 
BstYI r'GATC_y

Cuts at:      0      43    1713 
   Size:         43    1670
 
BtgI C'CryG_G

Cuts at:      0     372     566    1713 
   Size:        372     194    1147

  Fragments arranged by size: 

                1147     372     194
 
EaeI y'GGCC_r

Cuts at:      0      50     434     576    1713 
   Size:         50     384     142    1137

  Fragments arranged by size: 

                1137     384     142      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     716    1713 
   Size:        716     997
 
EcoICRI GAG'CTC

Cuts at:      0     156    1713 
   Size:        156    1557
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1571    1713 
   Size:        799     772     142

  Fragments arranged by size: 

                 799     772     142
 
EcoRV GAT'ATC

Cuts at:      0    1221    1713 
   Size:       1221     492
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     730    1713 
   Size:        730     983
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     218     250     368     400    1210    1242    1713 
   Size:        218      32     118      32     810      32     471

  Fragments arranged by size: 

                 810     471     218     118      32      32      32
 
HincII GTy'rAC

Cuts at:      0     548    1713 
   Size:        548    1165
 
HindIII A'AGCT_T

Cuts at:      0    1553    1713 
   Size:       1553     160
 
HpaI GTT'AAC

Cuts at:      0     548    1713 
   Size:        548    1165
 
MscI TGG'CCA

Cuts at:      0     436     578    1713 
   Size:        436     142    1135

  Fragments arranged by size: 

                1135     436     142
 
MslI CAynn'nnrTG

Cuts at:      0     328     350     649    1713 
   Size:        328      22     299    1064

  Fragments arranged by size: 

                1064     328     299      22
 
MspA1I CmG'CkG

Cuts at:      0     744    1713 
   Size:        744     969
 
NcoI C'CATG_G

Cuts at:      0     372     566    1713 
   Size:        372     194    1147

  Fragments arranged by size: 

                1147     372     194
 
NspI r_CATG'y

Cuts at:      0    1296    1713 
   Size:       1296     417
 
PciI A'CATG_T

Cuts at:      0    1292    1713 
   Size:       1292     421
 
PfoI T'CCnGG_A

Cuts at:      0    1325    1713 
   Size:       1325     388
 
PshAI GACnn'nnGTC

Cuts at:      0     184    1713 
   Size:        184    1529
 
PsiI TTA'TAA

Cuts at:      0    1474    1713 
   Size:       1474     239
 
PvuII CAG'CTG

Cuts at:      0     744    1713 
   Size:        744     969
 
SacI G_AGCT'C

Cuts at:      0     158    1713 
   Size:        158    1555
 
ScaI AGT'ACT

Cuts at:      0     441    1713 
   Size:        441    1272
 
SfcI C'TryA_G

Cuts at:      0    1070    1713 
   Size:       1070     643
 
SspI AAT'ATT

Cuts at:      0     423    1320    1713 
   Size:        423     897     393

  Fragments arranged by size: 

                 897     423     393
 
StyI C'CwwG_G

Cuts at:      0      47      87     372     566    1713 
   Size:         47      40     285     194    1147

  Fragments arranged by size: 

                1147     285     194      47      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234     787    1390    1713 
   Size:        234     553     603     323

  Fragments arranged by size: 

                 603     553     323     234
 
TatI w'GTAC_w

Cuts at:      0     439    1482    1713 
   Size:        439    1043     231

  Fragments arranged by size: 

                1043     439     231
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AloI    AlwNI     ApoI    BamHI    BanII
     BbsI    BciVI     BclI     BglI     BlpI Bme1580I     BmrI     BpmI
     BsaI    BsaHI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI
   BstAPI    BstXI    BstYI     BtgI     EaeI     EarI  EcoICRI  Eco57MI
    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI     MscI     MslI
   MspA1I     NcoI     NspI     PciI     PfoI    PshAI     PsiI    PvuII
     SacI     ScaI     SfcI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BfrBI    BglII
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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