Restriction map for pF1KB8669
 (Linear) (Six-base) MAPSORT of: KIBB8669.seq  Check: 8782  from: 1  to: 1770



KIBB8669.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     759    1770 
   Size:        759    1011
 
Acc65I G'GTAC_C

Cuts at:      0     674     923    1770 
   Size:        674     249     847

  Fragments arranged by size: 

                 847     674     249
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1134    1465    1770 
   Size:       1134     331     305

  Fragments arranged by size: 

                1134     331     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0     508     586     711     871     969    1770 
   Size:        508      78     125     160      98     801

  Fragments arranged by size: 

                 801     508     160     125      98      78
 
ApaI G_GGCC'C

Cuts at:      0     865    1615    1770 
   Size:        865     750     155

  Fragments arranged by size: 

                 865     750     155
 
ApaLI G'TGCA_C

Cuts at:      0    1063    1770 
   Size:       1063     707
 
AvaI C'yCGr_G

Cuts at:      0     131    1522    1770 
   Size:        131    1391     248

  Fragments arranged by size: 

                1391     248     131
 
AvrII C'CTAG_G

Cuts at:      0     297    1770 
   Size:        297    1473
 
BamHI G'GATC_C

Cuts at:      0     956    1770 
   Size:        956     814
 
BanI G'GyrC_C

Cuts at:      0      95     240     341     660     674     891     923    1113 
   Size:         95     145     101     319      14     217      32     190

Cuts at:   1113    1337    1770 
   Size:        224     433

  Fragments arranged by size: 

                 433     319     224     217     190     145     101      95
                  32      14
 
BanII G_rGCy'C

Cuts at:      0     476     865    1304    1547    1615    1770 
   Size:        476     389     439     243      68     155

  Fragments arranged by size: 

                 476     439     389     243     155      68
 
BbeI G_GCGC'C

Cuts at:      0    1341    1770 
   Size:       1341     429
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1078    1080    1770 
   Size:       1078       2     690

  Fragments arranged by size: 

                1078     690       2
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     509    1770 
   Size:        509    1261
 
BclI T'GATC_A

Cuts at:      0    1424    1770 
   Size:       1424     346
 
BglI GCCn_nnn'nGGC

Cuts at:      0     329     365     842    1129    1610    1770 
   Size:        329      36     477     287     481     160

  Fragments arranged by size: 

                 481     477     329     287     160      36
 
BglII A'GATC_T

Cuts at:      0    1019    1290    1770 
   Size:       1019     271     480

  Fragments arranged by size: 

                1019     480     271
 
BlpI GC'TnA_GC

Cuts at:      0     428    1546    1770 
   Size:        428    1118     224

  Fragments arranged by size: 

                1118     428     224
 
Bme1580I G_kGCm'C

Cuts at:      0      39     245     344     633     865     894    1067    1118 
   Size:         39     206      99     289     232      29     173      51

Cuts at:   1118    1583    1615    1770 
   Size:        465      32     155

  Fragments arranged by size: 

                 465     289     232     206     173     155      99      51
                  39      32      29
 
BmgBI CAC'GTC

Cuts at:      0    1515    1770 
   Size:       1515     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     602     620     627    1656    1770 
   Size:        602      18       7    1029     114

  Fragments arranged by size: 

                1029     602     114      18       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     582    1293    1770 
   Size:        582     711     477

  Fragments arranged by size: 

                 711     582     477
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     794     893    1240    1275    1770 
   Size:        457     337      99     347      35     495

  Fragments arranged by size: 

                 495     457     347     337      99      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     470     557    1770 
   Size:        470      87    1213

  Fragments arranged by size: 

                1213     470      87
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      54    1770 
   Size:         54    1716
 
BsaAI yAC'GTr

Cuts at:      0    1410    1770 
   Size:       1410     360
 
BsaHI Gr'CG_yC

Cuts at:      0    1338    1440    1770 
   Size:       1338     102     330

  Fragments arranged by size: 

                1338     330     102
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     754    1770 
   Size:        754    1016
 
BseYI C'CCAG_C

Cuts at:      0     236     378     529     678     701     719     730    1770 
   Size:        236     142     151     149      23      18      11    1040

  Fragments arranged by size: 

                1040     236     151     149     142      23      18      11
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1129    1770 
   Size:       1129     641
 
BsiEI CG_ry'CG

Cuts at:      0    1334    1638    1770 
   Size:       1334     304     132

  Fragments arranged by size: 

                1334     304     132
 
BsiHKAI G_wGCw'C

Cuts at:      0    1067    1304    1646    1688    1770 
   Size:       1067     237     342      42      82

  Fragments arranged by size: 

                1067     342     237      82      42
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1090    1770 
   Size:       1090     680
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     245     344     476     633     865     894    1067 
   Size:         39     206      99     132     157     232      29     173

Cuts at:   1067    1118    1304    1547    1583    1615    1646    1688    1770 
   Size:         51     186     243      36      32      31      42      82

  Fragments arranged by size: 

                 243     232     206     186     173     157     132      99
                  82      51      42      39      36      32      31      29
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     636     759    1034    1770 
   Size:        636     123     275     736

  Fragments arranged by size: 

                 736     636     275     123
 
BsrBI CCG'CTC

Cuts at:      0     854    1251    1770 
   Size:        854     397     519

  Fragments arranged by size: 

                 854     519     397
 
BsrFI r'CCGG_y

Cuts at:      0      29     845    1770 
   Size:         29     816     925

  Fragments arranged by size: 

                 925     816      29
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     832    1770 
   Size:        832     938
 
BstYI r'GATC_y

Cuts at:      0     956    1019    1290    1770 
   Size:        956      63     271     480

  Fragments arranged by size: 

                 956     480     271      63
 
BtgI C'CryG_G

Cuts at:      0    1328    1556    1679    1770 
   Size:       1328     228     123      91

  Fragments arranged by size: 

                1328     228     123      91
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1393    1536    1770 
   Size:       1393     143     234

  Fragments arranged by size: 

                1393     234     143
 
BtsI GCAGTG_nn'

Cuts at:      0     275     527     741    1770 
   Size:        275     252     214    1029

  Fragments arranged by size: 

                1029     275     252     214
 
DraIII CAC_nnn'GTG

Cuts at:      0     898    1770 
   Size:        898     872
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1012    1770 
   Size:       1012     758
 
EaeI y'GGCC_r

Cuts at:      0     366     524     843     900    1121    1194    1226    1331 
   Size:        366     158     319      57     221      73      32     105

Cuts at:   1331    1635    1661    1770 
   Size:        304      26     109

  Fragments arranged by size: 

                 366     319     304     221     158     109     105      73
                  57      32      26
 
EagI C'GGCC_G

Cuts at:      0    1331    1635    1770 
   Size:       1331     304     135

  Fragments arranged by size: 

                1331     304     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     220    1770 
   Size:        220    1550
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     347     864    1770 
   Size:        347     517     906

  Fragments arranged by size: 

                 906     517     347
 
EcoICRI GAG'CTC

Cuts at:      0    1302    1770 
   Size:       1302     468
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     794     893    1134    1240    1275    1465    1770 
   Size:        457     337      99     241     106      35     190     305

  Fragments arranged by size: 

                 457     337     305     241     190     106      99      35
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     301     861     966    1612    1770 
   Size:        301     560     105     646     158

  Fragments arranged by size: 

                 646     560     301     158     105
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1309    1770 
   Size:       1309     461
 
FspI TGC'GCA

Cuts at:      0     289    1770 
   Size:        289    1481
 
HaeII r_GCGC'y

Cuts at:      0     747    1341    1504    1770 
   Size:        747     594     163     266

  Fragments arranged by size: 

                 747     594     266     163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510    1159    1191    1770 
   Size:        478      32     649      32     579

  Fragments arranged by size: 

                 649     579     478      32      32
 
KasI G'GCGC_C

Cuts at:      0    1337    1770 
   Size:       1337     433
 
KpnI G_GTAC'C

Cuts at:      0     678     927    1770 
   Size:        678     249     843

  Fragments arranged by size: 

                 843     678     249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1770 
   Size:       1016     754
 
MscI TGG'CCA

Cuts at:      0     368    1663    1770 
   Size:        368    1295     107

  Fragments arranged by size: 

                1295     368     107
 
MslI CAynn'nnrTG

Cuts at:      0     154     395     401    1555    1770 
   Size:        154     241       6    1154     215

  Fragments arranged by size: 

                1154     241     215     154       6
 
MspA1I CmG'CkG

Cuts at:      0     529     945    1770 
   Size:        529     416     825

  Fragments arranged by size: 

                 825     529     416
 
NaeI GCC'GGC

Cuts at:      0      31     847    1770 
   Size:         31     816     923

  Fragments arranged by size: 

                 923     816      31
 
NarI GG'CG_CC

Cuts at:      0    1338    1770 
   Size:       1338     432
 
NgoMIV G'CCGG_C

Cuts at:      0      29     845    1770 
   Size:         29     816     925

  Fragments arranged by size: 

                 925     816      29
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     871     948    1770 
   Size:        871      77     822

  Fragments arranged by size: 

                 871     822      77
 
PfoI T'CCnGG_A

Cuts at:      0     223    1217    1770 
   Size:        223     994     553

  Fragments arranged by size: 

                 994     553     223
 
PspOMI G'GGCC_C

Cuts at:      0     861    1611    1770 
   Size:        861     750     159

  Fragments arranged by size: 

                 861     750     159
 
PstI C_TGCA'G

Cuts at:      0     513     965    1770 
   Size:        513     452     805

  Fragments arranged by size: 

                 805     513     452
 
PvuII CAG'CTG

Cuts at:      0     945    1770 
   Size:        945     825
 
SacI G_AGCT'C

Cuts at:      0    1304    1770 
   Size:       1304     466
 
SbfI CC_TGCA'GG

Cuts at:      0     965    1770 
   Size:        965     805
 
SexAI A'CCwGG_T

Cuts at:      0     670    1770 
   Size:        670    1100
 
SfcI C'TryA_G

Cuts at:      0     509     961    1770 
   Size:        509     452     809

  Fragments arranged by size: 

                 809     509     452
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     365     842    1129    1770 
   Size:        365     477     287     641

  Fragments arranged by size: 

                 641     477     365     287
 
SfoI GGC'GCC

Cuts at:      0    1339    1770 
   Size:       1339     431
 
SmaI CCC'GGG

Cuts at:      0     133    1770 
   Size:        133    1637
 
StuI AGG'CCT

Cuts at:      0     323    1417    1770 
   Size:        323    1094     353

  Fragments arranged by size: 

                1094     353     323
 
StyI C'CwwG_G

Cuts at:      0     297     983    1058    1770 
   Size:        297     686      75     712

  Fragments arranged by size: 

                 712     686     297      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     128     159     193     228     352     678     715    1770 
   Size:        128      31      34      35     124     326      37    1055

  Fragments arranged by size: 

                1055     326     128     124      37      35      34      31
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     352    1770 
   Size:        352    1418
 
XmaI C'CCGG_G

Cuts at:      0     131    1770 
   Size:        131    1639
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     ApaI    ApaLI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
    PflMI     PfoI   PspOMI     PstI    PvuII     SacI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmaI     StuI     StyI    TaqII     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI     BaeI
    BbvCI     BcgI    BfrBI     BmtI    BpuEI    BsaBI    BsaWI    BsaXI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
    EcoNI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     ScaI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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