Restriction map for
pF1KB8661
(Circular) (Six-base) MAPSORT of: pF1KB8661.seq Check: 3824 from: 1 to: 4373
pF1KB8661 4373 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1411 1411
Size: 4373
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 2198 2630 2983 3645 332
Size: 1866 432 353 662 1060
Fragments arranged by size:
1866 1060 662 432 353
AflIII A'CryG_T
Cuts at: 184 1253 1725 3525 3806 184
Size: 1069 472 1800 281 751
Fragments arranged by size:
1800 1069 751 472 281
AleI CACnn'nnGTG
Cuts at: 895 895
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 1069 1678 3116 1069
Size: 609 1438 2326
Fragments arranged by size:
2326 1438 609
ApaLI G'TGCA_C
Cuts at: 3211 3211
Size: 4373
ApoI r'AATT_y
Cuts at: 1377 3726 3982 1377
Size: 2349 256 1768
Fragments arranged by size:
2349 1768 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 1393 1393
Size: 4373
BamHI G'GATC_C
Cuts at: 1398 1398
Size: 4373
BanI G'GyrC_C
Cuts at: 278 1389 1803 2051 2086 278
Size: 1111 414 248 35 2565
Fragments arranged by size:
2565 1111 414 248 35
BanII G_rGCy'C
Cuts at: 1110 1296 1344 1387 2417 1110
Size: 186 48 43 1030 3066
Fragments arranged by size:
3066 1030 186 48 43
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BbsI GAAGACnn'nnnn_
Cuts at: 313 313
Size: 4373
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3327 4373 2271
Size: 1056 1046 2271
Fragments arranged by size:
2271 1056 1046
BclI T'GATC_A
Cuts at: 690 1893 690
Size: 1203 3170
BglII A'GATC_T
Cuts at: 1888 3925 1888
Size: 2037 2336
BlpI GC'TnA_GC
Cuts at: 1485 1485
Size: 4373
Bme1580I G_kGCm'C
Cuts at: 283 1998 2091 3215 283
Size: 1715 93 1124 1441
Fragments arranged by size:
1715 1441 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 159 1585 1666 2001 4145 159
Size: 1426 81 335 2144 387
Fragments arranged by size:
2144 1426 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 1163 1280 305
Size: 858 117 3398
Fragments arranged by size:
3398 858 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1549 2428 3142 3440 1549
Size: 879 714 298 2482
Fragments arranged by size:
2482 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4077 4077
Size: 4373
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 881 1562 2083 2761 3172 3319 3593 881
Size: 681 521 678 411 147 274 1661
Fragments arranged by size:
1661 681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 586 616 586
Size: 30 4343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 647 1106 1109 647
Size: 459 3 3911
Fragments arranged by size:
3911 459 3
BseYI C'CCAG_C
Cuts at: 516 1798 2556 3221 3629 516
Size: 1282 758 665 408 1260
Fragments arranged by size:
1282 1260 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 919
Size: 4373
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3191 104
Size: 1811 46 1230 1286
Fragments arranged by size:
1811 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1110 1344 1387 2165 2355 3215 1110
Size: 234 43 778 190 860 2268
Fragments arranged by size:
2268 860 778 234 190 43
BsmI GAATG_Cn'
Cuts at: 244 244
Size: 4373
Bsp1286I G_dGCh'C
Cuts at: 283 1110 1296 1344 1387 1998 2091 2165 2355
Size: 827 186 48 43 611 93 74 190
Cuts at: 2355 2417 3215 283
Size: 62 798 1441
Fragments arranged by size:
1441 827 798 611 190 186 93 74
62 48 43
BspEI T'CCGG_A
Cuts at: 881 881
Size: 4373
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 364 1423 1637 1939 2320 334
Size: 30 1059 214 302 381 2387
Fragments arranged by size:
2387 1059 381 302 214 30
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1193 1217 2285 1193
Size: 24 1068 3281
Fragments arranged by size:
3281 1068 24
BsrFI r'CCGG_y
Cuts at: 2371 2552 2371
Size: 181 4192
BssSI C'ACGA_G
Cuts at: 2644 3352 2644
Size: 708 3665
BstAPI GCAn_nnn'nTGC
Cuts at: 1750 1750
Size: 4373
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstYI r'GATC_y
Cuts at: 1 669 878 1018 1398 1865 1888 2223 2469
Size: 668 209 140 380 467 23 335 246
Cuts at: 2469 2775 2787 2873 3925 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 668 467 449 380 335 306 246
209 140 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 916 1616 916
Size: 700 3673
DraI TTT'AAA
Cuts at: 417 1372 417
Size: 955 3418
DrdI GACnn_nn'nnGTC
Cuts at: 2079 3423 2079
Size: 1344 3029
EaeI y'GGCC_r
Cuts at: 502 990 1958 2132 2523 2550 3895 502
Size: 488 968 174 391 27 1345 980
Fragments arranged by size:
1345 980 968 488 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 1115 2396 2606 1115
Size: 1281 210 2882
Fragments arranged by size:
2882 1281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 3323 3469 4183 2581
Size: 742 146 714 2771
Fragments arranged by size:
2771 742 714 146
EcoICRI GAG'CTC
Cuts at: 1108 1342 1385 1108
Size: 234 43 4096
Fragments arranged by size:
4096 234 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 332 1163 1280 2198 2630 2983 3645 305
Size: 27 831 117 918 432 353 662 1033
Fragments arranged by size:
1033 918 831 662 432 353 117 27
EcoNI CCTnn'n_nnAGG
Cuts at: 138 1091 138
Size: 953 3420
EcoO109I rG'GnC_Cy
Cuts at: 1323 1512 1323
Size: 189 4184
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4373
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 1807 2055 3285 4056 1807
Size: 248 1230 771 2124
Fragments arranged by size:
2124 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1326 1358 2215 2245 2247 2277 1326
Size: 32 857 30 2 30 3422
Fragments arranged by size:
3422 857 32 30 30 2
HincII GTy'rAC
Cuts at: 444 1412 444
Size: 968 3405
HindIII A'AGCT_T
Cuts at: 742 742
Size: 4373
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 152 3132 3316 152
Size: 2980 184 1209
Fragments arranged by size:
2980 1209 184
MscI TGG'CCA
Cuts at: 2134 2134
Size: 4373
MslI CAynn'nnrTG
Cuts at: 895 2362 2489 895
Size: 1467 127 2779
Fragments arranged by size:
2779 1467 127
MspA1I CmG'CkG
Cuts at: 1183 1484 1798 2158 2942 3187 1183
Size: 301 314 360 784 245 2369
Fragments arranged by size:
2369 784 360 314 301 245
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NcoI C'CATG_G
Cuts at: 107 107
Size: 4373
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NspI r_CATG'y
Cuts at: 188 297 429 516 546 1257 1426 1926 3529
Size: 109 132 87 30 711 169 500 1603
Cuts at: 3529 188
Size: 1032
Fragments arranged by size:
1603 1032 711 500 169 132 109 87
30
PciI A'CATG_T
Cuts at: 184 1253 3525 184
Size: 1069 2272 1032
Fragments arranged by size:
2272 1069 1032
PflMI CCAn_nnn'nTGG
Cuts at: 1243 1266 1243
Size: 23 4350
PfoI T'CCnGG_A
Cuts at: 1092 1296 1092
Size: 204 4169
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 4341
PpuMI rG'GwC_Cy
Cuts at: 1323 1323
Size: 4373
PstI C_TGCA'G
Cuts at: 331 948 1128 1420 2105 331
Size: 617 180 292 685 2599
Fragments arranged by size:
2599 685 617 292 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 1183 1798 2158 1183
Size: 615 360 3398
Fragments arranged by size:
3398 615 360
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1110 1344 1387 1110
Size: 234 43 4096
Fragments arranged by size:
4096 234 43
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 1115 2396 2606 1115
Size: 1281 210 2882
Fragments arranged by size:
2882 1281 210
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 454 3890 454
Size: 3436 937
SfcI C'TryA_G
Cuts at: 33 327 665 731 944 1061 1124 1416 2101
Size: 294 338 66 213 117 63 292 685
Cuts at: 2101 3069 3260 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 338 294 292 213 191
117 66 63
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 1528 2443 3157 3419 1528
Size: 915 714 262 2482
Fragments arranged by size:
2482 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 297 516 1426 1926 297
Size: 219 910 500 2744
Fragments arranged by size:
2744 910 500 219
SspI AAT'ATT
Cuts at: 699 699
Size: 4373
StyI C'CwwG_G
Cuts at: 107 1507 1861 107
Size: 1400 354 2619
Fragments arranged by size:
2619 1400 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 600 1277 1956 2089 2548 2757 600
Size: 677 679 133 459 209 2216
Fragments arranged by size:
2216 679 677 459 209 133
TatI w'GTAC_w
Cuts at: 452 3888 452
Size: 3436 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 586 618 1241 1273 3890 3922 586
Size: 32 623 32 2617 32 1037
Fragments arranged by size:
2617 1037 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 1868 187
Size: 1681 2692
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
XmnI GAAnn'nnTTC
Cuts at: 1088 1088
Size: 4373
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI