Restriction map for pF1KB8661
 (Linear) (Six-base) MAPSORT of: KIBB8661.seq  Check: 1197  from: 1  to: 1260



KIBB8661.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224    1260 
   Size:        224    1036
 
AflIII A'CryG_T

Cuts at:      0      76    1145    1260 
   Size:         76    1069     115

  Fragments arranged by size: 

                1069     115      76
 
AleI CACnn'nnGTG

Cuts at:      0     787    1260 
   Size:        787     473
 
AlwNI CAG_nnn'CTG

Cuts at:      0     961    1260 
   Size:        961     299
 
BanI G'GyrC_C

Cuts at:      0     170    1260 
   Size:        170    1090
 
BanII G_rGCy'C

Cuts at:      0    1002    1188    1236    1260 
   Size:       1002     186      48      24

  Fragments arranged by size: 

                1002     186      48      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     205    1260 
   Size:        205    1055
 
BclI T'GATC_A

Cuts at:      0     582    1260 
   Size:        582     678
 
Bme1580I G_kGCm'C

Cuts at:      0     175    1260 
   Size:        175    1085
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      51    1260 
   Size:         51    1209
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197    1055    1172    1260 
   Size:        197     858     117      88

  Fragments arranged by size: 

                 858     197     117      88
 
BsaWI w'CCGG_w

Cuts at:      0     773    1260 
   Size:        773     487
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     478     508    1260 
   Size:        478      30     752

  Fragments arranged by size: 

                 752     478      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     539     998    1001    1260 
   Size:        539     459       3     259

  Fragments arranged by size: 

                 539     459     259       3
 
BseYI C'CCAG_C

Cuts at:      0     408    1260 
   Size:        408     852
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1260 
   Size:        811     449
 
BsiHKAI G_wGCw'C

Cuts at:      0    1002    1236    1260 
   Size:       1002     234      24

  Fragments arranged by size: 

                1002     234      24
 
BsmI GAATG_Cn'

Cuts at:      0     136    1260 
   Size:        136    1124
 
Bsp1286I G_dGCh'C

Cuts at:      0     175    1002    1188    1236    1260 
   Size:        175     827     186      48      24

  Fragments arranged by size: 

                 827     186     175      48      24
 
BspEI T'CCGG_A

Cuts at:      0     773    1260 
   Size:        773     487
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     226     256    1260 
   Size:        226      30    1004

  Fragments arranged by size: 

                1004     226      30
 
BsrDI GCAATG_nn'

Cuts at:      0    1085    1109    1260 
   Size:       1085      24     151

  Fragments arranged by size: 

                1085     151      24
 
BstYI r'GATC_y

Cuts at:      0     561     770     910    1260 
   Size:        561     209     140     350

  Fragments arranged by size: 

                 561     350     209     140
 
BtsI GCAGTG_nn'

Cuts at:      0     808    1260 
   Size:        808     452
 
DraI TTT'AAA

Cuts at:      0     309    1260 
   Size:        309     951
 
EaeI y'GGCC_r

Cuts at:      0     394     882    1260 
   Size:        394     488     378

  Fragments arranged by size: 

                 488     394     378
 
EarI CTCTTCn'nnn_

Cuts at:      0    1007    1260 
   Size:       1007     253
 
EcoICRI GAG'CTC

Cuts at:      0    1000    1234    1260 
   Size:       1000     234      26

  Fragments arranged by size: 

                1000     234      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     224    1055    1172    1260 
   Size:        197      27     831     117      88

  Fragments arranged by size: 

                 831     197     117      88      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      30     983    1260 
   Size:         30     953     277

  Fragments arranged by size: 

                 953     277      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1215    1260 
   Size:       1215      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1218    1250    1260 
   Size:       1218      32      10

  Fragments arranged by size: 

                1218      32      10
 
HincII GTy'rAC

Cuts at:      0     336    1260 
   Size:        336     924
 
HindIII A'AGCT_T

Cuts at:      0     634    1260 
   Size:        634     626
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      44    1260 
   Size:         44    1216
 
MslI CAynn'nnrTG

Cuts at:      0     787    1260 
   Size:        787     473
 
MspA1I CmG'CkG

Cuts at:      0    1075    1260 
   Size:       1075     185
 
NspI r_CATG'y

Cuts at:      0      80     189     321     408     438    1149    1260 
   Size:         80     109     132      87      30     711     111

  Fragments arranged by size: 

                 711     132     111     109      87      80      30
 
PciI A'CATG_T

Cuts at:      0      76    1145    1260 
   Size:         76    1069     115

  Fragments arranged by size: 

                1069     115      76
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1135    1158    1260 
   Size:       1135      23     102

  Fragments arranged by size: 

                1135     102      23
 
PfoI T'CCnGG_A

Cuts at:      0     984    1188    1260 
   Size:        984     204      72

  Fragments arranged by size: 

                 984     204      72
 
PpuMI rG'GwC_Cy

Cuts at:      0    1215    1260 
   Size:       1215      45
 
PstI C_TGCA'G

Cuts at:      0     223     840    1020    1260 
   Size:        223     617     180     240

  Fragments arranged by size: 

                 617     240     223     180
 
PvuII CAG'CTG

Cuts at:      0    1075    1260 
   Size:       1075     185
 
SacI G_AGCT'C

Cuts at:      0    1002    1236    1260 
   Size:       1002     234      24

  Fragments arranged by size: 

                1002     234      24
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1007    1260 
   Size:       1007     253
 
ScaI AGT'ACT

Cuts at:      0     346    1260 
   Size:        346     914
 
SfcI C'TryA_G

Cuts at:      0     219     557     623     836     953    1016    1260 
   Size:        219     338      66     213     117      63     244

  Fragments arranged by size: 

                 338     244     219     213     117      66      63
 
SphI G_CATG'C

Cuts at:      0     189     408    1260 
   Size:        189     219     852

  Fragments arranged by size: 

                 852     219     189
 
SspI AAT'ATT

Cuts at:      0     591    1260 
   Size:        591     669
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     492    1169    1260 
   Size:        492     677      91

  Fragments arranged by size: 

                 677     492      91
 
TatI w'GTAC_w

Cuts at:      0     344    1260 
   Size:        344     916
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     478     510    1133    1165    1260 
   Size:        478      32     623      32      95

  Fragments arranged by size: 

                 623     478      95      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      79    1260 
   Size:         79    1181
 
XmnI GAAnn'nnTTC

Cuts at:      0     980    1260 
   Size:        980     280
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     BanI    BanII     BbsI     BclI
 Bme1580I     BmrI     BpmI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BstYI     BtsI
     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     NspI     PciI    PflMI
     PfoI    PpuMI     PstI    PvuII     SacI     SapI     ScaI     SfcI
     SphI     SspI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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