Restriction map for pF1KB8658
 (Circular) (Six-base) MAPSORT of: pF1KB8658.seq  Check: 5705  from: 1  to: 4673



pF1KB8658  4673 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1668    1668 
   Size:       4673
 
AatII G_ACGT'C

Cuts at:   1536    1536 
   Size:       4673
 
AccI GT'mk_AC

Cuts at:    479     932    1530    1711     479 
   Size:        453     598     181    3441

  Fragments arranged by size: 

                3441     598     453     181
 
Acc65I G'GTAC_C

Cuts at:   1689    1689 
   Size:       4673
 
AclI AA'CG_TT

Cuts at:   4513    4513 
   Size:       4673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    947    2498    2930    3677    3945     947 
   Size:       1551     432     747     268    1675

  Fragments arranged by size: 

                1675    1551     747     432     268
 
AflIII A'CryG_T

Cuts at:   2025    3129    4106    2025 
   Size:       1104     977    2592

  Fragments arranged by size: 

                2592    1104     977
 
AgeI A'CCGG_T

Cuts at:   3061    3061 
   Size:       4673
 
AlwNI CAG_nnn'CTG

Cuts at:    412    1978    3545     412 
   Size:       1566    1567    1540

  Fragments arranged by size: 

                1567    1566    1540
 
ApaLI G'TGCA_C

Cuts at:   3443    3443 
   Size:       4673
 
ApoI r'AATT_y

Cuts at:   1677    4026    4282    1677 
   Size:       2349     256    2068

  Fragments arranged by size: 

                2349    2068     256
 
AseI AT'TA_AT

Cuts at:     20     503      20 
   Size:        483    4190
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
AvaI C'yCGr_G

Cuts at:   1693    1693 
   Size:       4673
 
BamHI G'GATC_C

Cuts at:   1698    1698 
   Size:       4673
 
BanI G'GyrC_C

Cuts at:   1689    2103    2351    2386    1689 
   Size:        414     248      35    3976

  Fragments arranged by size: 

                3976     414     248      35
 
BanII G_rGCy'C

Cuts at:    978    1687    2717     978 
   Size:        709    1030    2934

  Fragments arranged by size: 

                2934    1030     709
 
BbeI G_GCGC'C

Cuts at:   2107    2355    2107 
   Size:        248    4425
 
BbsI GAAGACnn'nnnn_

Cuts at:   1505    1505 
   Size:       4673
 
BciVI GTATCCnnnnn_n'

Cuts at:    638    2571    3332    4673     638 
   Size:       1933     761    1341     638

  Fragments arranged by size: 

                1933    1341     761     638
 
BclI T'GATC_A

Cuts at:   1353    2193    1353 
   Size:        840    3833
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4673
 
BglII A'GATC_T

Cuts at:    916    2188    4225     916 
   Size:       1272    2037    1364

  Fragments arranged by size: 

                2037    1364    1272
 
BlpI GC'TnA_GC

Cuts at:    145    1785     145 
   Size:       1640    3033
 
Bme1580I G_kGCm'C

Cuts at:   2298    2391    3447    2298 
   Size:         93    1056    3524

  Fragments arranged by size: 

                3524    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    800    1885    1966    2301    4445     800 
   Size:       1085      81     335    2144    1028

  Fragments arranged by size: 

                2144    1085    1028     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    532    3040     532 
   Size:       2508    2165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    953     953 
   Size:       4673
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1849    2728    3220    3518    1849 
   Size:        879     492     298    3004

  Fragments arranged by size: 

                3004     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    295    1292    4377     295 
   Size:        997    3085     591

  Fragments arranged by size: 

                3085     997     591
 
BsaAI yAC'GTr

Cuts at:   1150    2026    1150 
   Size:        876    3797
 
BsaBI GATnn'nnATC

Cuts at:      6    2187    4224       6 
   Size:       2181    2037     455

  Fragments arranged by size: 

                2181    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1533    2104    2352    3054    4550    1533 
   Size:        571     248     702    1496    1656

  Fragments arranged by size: 

                1656    1496     702     571     248
 
BsaWI w'CCGG_w

Cuts at:    157    1862    2383    3061    3335    3482    3893     157 
   Size:       1705     521     678     274     147     411     937

  Fragments arranged by size: 

                1705     937     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    163     193     387     417     756     786     163 
   Size:         30     194      30     339      30    4050

  Fragments arranged by size: 

                4050     339     194      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    184     761     968     989    1007    1058     184 
   Size:        577     207      21      18      51    3799

  Fragments arranged by size: 

                3799     577     207      51      21      18
 
BseYI C'CCAG_C

Cuts at:   2098    2856    3433    3929    2098 
   Size:        758     577     496    2842

  Fragments arranged by size: 

                2842     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1130    1130 
   Size:       4673
 
BsiEI CG_ry'CG

Cuts at:    104     116    2215    2261    3469     104 
   Size:         12    2099      46    1208    1308

  Fragments arranged by size: 

                2099    1308    1208      46      12
 
BsiHKAI G_wGCw'C

Cuts at:    384     978    1648    1687    2465    2655    3447     384 
   Size:        594     670      39     778     190     792    1610

  Fragments arranged by size: 

                1610     792     778     670     594     190      39
 
Bsp1286I G_dGCh'C

Cuts at:    384     978    1648    1687    2298    2391    2465    2655    2717 
   Size:        594     670      39     611      93      74     190      62

Cuts at:   2717    3447     384 
   Size:        730    1610

  Fragments arranged by size: 

                1610     730     670     611     594     190      93      74
                  62      39
 
BspEI T'CCGG_A

Cuts at:   3893    3893 
   Size:       4673
 
BspHI T'CATG_A

Cuts at:   3849    3849 
   Size:       4673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1668    1723    1937    2239    2620    1668 
   Size:         55     214     302     381    3721

  Fragments arranged by size: 

                3721     381     302     214      55
 
BsrBI CCG'CTC

Cuts at:     50     149    2965    3019    4503    4668      50 
   Size:         99    2816      54    1484     165      55

  Fragments arranged by size: 

                2816    1484     165      99      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1173    2585    1173 
   Size:       1412    3261
 
BsrFI r'CCGG_y

Cuts at:    115    2671    2852    3061     115 
   Size:       2556     181     209    1727

  Fragments arranged by size: 

                2556    1727     209     181
 
BssSI C'ACGA_G

Cuts at:   2944    3302    2944 
   Size:        358    4315
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2050    2050 
   Size:       4673
 
BstBI TT'CG_AA

Cuts at:   3034    3034 
   Size:       4673
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1189    1189 
   Size:       4673
 
BstYI r'GATC_y

Cuts at:      1     916    1698    2165    2188    2523    2769    3781    3867 
   Size:        915     782     467      23     335     246    1012      86

Cuts at:   3867    3879    4225       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     915     782     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    553     553 
   Size:       4673
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    206     344    1400    2623    2804    2967     206 
   Size:        138    1056    1223     181     163    1912

  Fragments arranged by size: 

                1912    1223    1056     181     163     138
 
BtsI GCAGTG_nn'

Cuts at:   1031    1240    1626    1916    1031 
   Size:        209     386     290    3788

  Fragments arranged by size: 

                3788     386     290     209
 
DraI TTT'AAA

Cuts at:   1672    1672 
   Size:       4673
 
DraIII CAC_nnn'GTG

Cuts at:   1372    1372 
   Size:       4673
 
DrdI GACnn_nn'nnGTC

Cuts at:   2379    3237    2379 
   Size:        858    3815
 
EaeI y'GGCC_r

Cuts at:    113    2258    2432    2823    2850    4195     113 
   Size:       2145     174     391      27    1345     591

  Fragments arranged by size: 

                2145    1345     591     391     174      27
 
EagI C'GGCC_G

Cuts at:    113    2258     113 
   Size:       2145    2528
 
EarI CTCTTCn'nnn_

Cuts at:    170     435    1248    2696    2906     170 
   Size:        265     813    1448     210    1937

  Fragments arranged by size: 

                1937    1448     813     265     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2881    3191    3337    4483    2881 
   Size:        310     146    1146    3071

  Fragments arranged by size: 

                3071    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    976    1685     976 
   Size:        709    3964
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    947     953    2498    2930    3677    3945     947 
   Size:          6    1545     432     747     268    1675

  Fragments arranged by size: 

                1675    1545     747     432     268       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1073    1073 
   Size:       4673
 
EcoO109I rG'GnC_Cy

Cuts at:    903    1812     903 
   Size:        909    3764
 
EcoRI G'AATT_C

Cuts at:   1677    1677 
   Size:       4673
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2692    2692 
   Size:       4673
 
FspI TGC'GCA

Cuts at:   2454    2454 
   Size:       4673
 
HaeII r_GCGC'y

Cuts at:    132    1082    2107    2355    3377    4356     132 
   Size:        950    1025     248    1022     979     449

  Fragments arranged by size: 

                1025    1022     979     950     449     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2515    2545    2547    2577    2515 
   Size:         30       2      30    4611

  Fragments arranged by size: 

                4611      30      30       2
 
HincII GTy'rAC

Cuts at:   1712    1712 
   Size:       4673
 
HindIII A'AGCT_T

Cuts at:    620     620 
   Size:       4673
 
KasI G'GCGC_C

Cuts at:   2103    2351    2103 
   Size:        248    4425
 
KpnI G_GTAC'C

Cuts at:   1693    1693 
   Size:       4673
 
MfeI C'AATT_G

Cuts at:    824     824 
   Size:       4673
 
MluI A'CGCG_T

Cuts at:   4106    4106 
   Size:       4673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    119    1045    3344    3528     119 
   Size:        926    2299     184    1264

  Fragments arranged by size: 

                2299    1264     926     184
 
MscI TGG'CCA

Cuts at:   2434    2434 
   Size:       4673
 
MslI CAynn'nnrTG

Cuts at:   1544    2662    2789    1544 
   Size:       1118     127    3428

  Fragments arranged by size: 

                3428    1118     127
 
MspA1I CmG'CkG

Cuts at:   1784    2098    2458    3471    3716    1784 
   Size:        314     360    1013     245    2741

  Fragments arranged by size: 

                2741    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    117    2854     117 
   Size:       2737    1936
 
NarI GG'CG_CC

Cuts at:   2104    2352    2104 
   Size:        248    4425
 
NcoI C'CATG_G

Cuts at:    553     553 
   Size:       4673
 
NdeI CA'TA_TG

Cuts at:   1161    1161 
   Size:       4673
 
NgoMIV G'CCGG_C

Cuts at:    115    2852     115 
   Size:       2737    1936
 
NspI r_CATG'y

Cuts at:   1726    2226    3133    1726 
   Size:        500     907    3266

  Fragments arranged by size: 

                3266     907     500
 
PciI A'CATG_T

Cuts at:   3129    3129 
   Size:       4673
 
PflMI CCAn_nnn'nTGG

Cuts at:    391     391 
   Size:       4673
 
PfoI T'CCnGG_A

Cuts at:    969     969 
   Size:       4673
 
PmeI GTTT'AAAC

Cuts at:   1672    1672 
   Size:       4673
 
PmlI CAC'GTG

Cuts at:   1150    1150 
   Size:       4673
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3812    3844    3812 
   Size:         32    4641
 
PpuMI rG'GwC_Cy

Cuts at:    903     903 
   Size:       4673
 
PshAI GACnn'nnGTC

Cuts at:    466    1509     466 
   Size:       1043    3630
 
PstI C_TGCA'G

Cuts at:   1665    1720    2405    1665 
   Size:         55     685    3933

  Fragments arranged by size: 

                3933     685      55
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4673
 
PvuII CAG'CTG

Cuts at:   2098    2458    2098 
   Size:        360    4313
 
RsrII CG'GwC_CG

Cuts at:   2868    2868 
   Size:       4673
 
SacI G_AGCT'C

Cuts at:    978    1687     978 
   Size:        709    3964
 
SalI G'TCGA_C

Cuts at:   1710    1710 
   Size:       4673
 
SapI GCTCTTCn'nnn_

Cuts at:   2696    2906    2696 
   Size:        210    4463
 
SbfI CC_TGCA'GG

Cuts at:   1720    1720 
   Size:       4673
 
ScaI AGT'ACT

Cuts at:   4190    4190 
   Size:       4673
 
SexAI A'CCwGG_T

Cuts at:    564     564 
   Size:       4673
 
SfcI C'TryA_G

Cuts at:     33     359     455    1661    1716    2401    3394    3585      33 
   Size:        326      96    1206      55     685     993     191    1121

  Fragments arranged by size: 

                1206    1121     993     685     326     191      96      55
            
 
SfoI GGC'GCC

Cuts at:   2105    2353    2105 
   Size:        248    4425
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
SmaI CCC'GGG

Cuts at:   1695    1695 
   Size:       4673
 
SmlI C'TyrA_G

Cuts at:   1828    2743    3235    3497    1828 
   Size:        915     492     262    3004

  Fragments arranged by size: 

                3004     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4673
 
SphI G_CATG'C

Cuts at:   1726    2226    1726 
   Size:        500    4173
 
StyI C'CwwG_G

Cuts at:    553    1807    2161     553 
   Size:       1254     354    3065

  Fragments arranged by size: 

                3065    1254     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    319     826    1280    2256    2389    2848    3057     319 
   Size:        507     454     976     133     459     209    1935

  Fragments arranged by size: 

                1935     976     507     459     454     209     133
 
TatI w'GTAC_w

Cuts at:   4188    4188 
   Size:       4673
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    163     195     975    1007    4190    4222     163 
   Size:         32     780      32    3183      32     614

  Fragments arranged by size: 

                3183     780     614      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2470    2470 
   Size:       4673
 
XbaI T'CTAG_A

Cuts at:   1704    1704 
   Size:       4673
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2168    2168 
   Size:       4673
 
XmaI C'CCGG_G

Cuts at:   1693    1693 
   Size:       4673
 
ZraI GAC'GTC

Cuts at:   1534    1534 
   Size:       4673
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI     NheI     NotI
     NruI     NsiI     PacI     PasI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI
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