Restriction map for
pF1KB8631
(Linear) (Six-base) MAPSORT of: KIBB8631.seq Check: 6903 from: 1 to: 1392
KIBB8631.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1380 1392
Size: 1380 12
AccI GT'mk_AC
Cuts at: 0 488 920 1113 1392
Size: 488 432 193 279
Fragments arranged by size:
488 432 279 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 602 653 935 1027 1392
Size: 143 459 51 282 92 365
Fragments arranged by size:
459 365 282 143 92 51
AleI CACnn'nnGTG
Cuts at: 0 607 1392
Size: 607 785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 1188 1392
Size: 150 1038 204
Fragments arranged by size:
1038 204 150
ApoI r'AATT_y
Cuts at: 0 638 1392
Size: 638 754
AvaI C'yCGr_G
Cuts at: 0 176 1392
Size: 176 1216
BamHI G'GATC_C
Cuts at: 0 225 1392
Size: 225 1167
BanII G_rGCy'C
Cuts at: 0 89 213 386 1392
Size: 89 124 173 1006
Fragments arranged by size:
1006 173 124 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 1392
Size: 93 1299
BbvCI CC'TCA_GC
Cuts at: 0 1307 1392
Size: 1307 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 1392
Size: 570 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1392
Size: 326 1066
Bpu10I CC'TnA_GC
Cuts at: 0 1307 1392
Size: 1307 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1071 1392
Size: 487 584 321
Fragments arranged by size:
584 487 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 1392
Size: 778 614
BsaAI yAC'GTr
Cuts at: 0 1098 1392
Size: 1098 294
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 71 101 270 300 1055 1085 1392
Size: 71 30 169 30 755 30 307
Fragments arranged by size:
755 307 169 71 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 1392
Size: 559 833
BseYI C'CCAG_C
Cuts at: 0 50 71 216 1392
Size: 50 21 145 1176
Fragments arranged by size:
1176 145 50 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1392
Size: 499 893
Bsp1286I G_dGCh'C
Cuts at: 0 89 213 386 1392
Size: 89 124 173 1006
Fragments arranged by size:
1006 173 124 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 1380 1392
Size: 521 859 12
Fragments arranged by size:
859 521 12
BsrDI GCAATG_nn'
Cuts at: 0 134 1392
Size: 134 1258
BsrFI r'CCGG_y
Cuts at: 0 7 493 1392
Size: 7 486 899
Fragments arranged by size:
899 486 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 522 1392
Size: 522 870
BstBI TT'CG_AA
Cuts at: 0 889 1392
Size: 889 503
BstYI r'GATC_y
Cuts at: 0 34 225 1125 1392
Size: 34 191 900 267
Fragments arranged by size:
900 267 191 34
Bsu36I CC'TnA_GG
Cuts at: 0 652 1392
Size: 652 740
BtgI C'CryG_G
Cuts at: 0 113 1392
Size: 113 1279
BtsI GCAGTG_nn'
Cuts at: 0 132 1392
Size: 132 1260
DrdI GACnn_nn'nnGTC
Cuts at: 0 373 928 1392
Size: 373 555 464
Fragments arranged by size:
555 464 373
EaeI y'GGCC_r
Cuts at: 0 288 1207 1392
Size: 288 919 185
Fragments arranged by size:
919 288 185
EarI CTCTTCn'nnn_
Cuts at: 0 53 893 1392
Size: 53 840 499
Fragments arranged by size:
840 499 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 326 602 653 935 1027 1392
Size: 143 183 276 51 282 92 365
Fragments arranged by size:
365 282 276 183 143 92 51
EcoO109I rG'GnC_Cy
Cuts at: 0 181 281 1392
Size: 181 100 1111
Fragments arranged by size:
1111 181 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1303 1392
Size: 1303 89
FspI TGC'GCA
Cuts at: 0 727 1392
Size: 727 665
FspAI rTGC'GCAy
Cuts at: 0 727 1392
Size: 727 665
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 430 462 777 809 894 926
Size: 71 32 327 32 315 32 85 32
Cuts at: 926 1055 1087 1392
Size: 129 32 305
Fragments arranged by size:
327 315 305 129 85 71 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 804 1392
Size: 804 588
MscI TGG'CCA
Cuts at: 0 290 1209 1392
Size: 290 919 183
Fragments arranged by size:
919 290 183
MslI CAynn'nnrTG
Cuts at: 0 409 607 1392
Size: 409 198 785
Fragments arranged by size:
785 409 198
MspA1I CmG'CkG
Cuts at: 0 71 216 346 443 1392
Size: 71 145 130 97 949
Fragments arranged by size:
949 145 130 97 71
NspI r_CATG'y
Cuts at: 0 726 1392
Size: 726 666
PpuMI rG'GwC_Cy
Cuts at: 0 181 281 1392
Size: 181 100 1111
Fragments arranged by size:
1111 181 100
PstI C_TGCA'G
Cuts at: 0 139 857 1164 1377 1392
Size: 139 718 307 213 15
Fragments arranged by size:
718 307 213 139 15
PvuII CAG'CTG
Cuts at: 0 71 346 443 1392
Size: 71 275 97 949
Fragments arranged by size:
949 275 97 71
ScaI AGT'ACT
Cuts at: 0 1048 1392
Size: 1048 344
SfcI C'TryA_G
Cuts at: 0 135 167 853 1160 1373 1392
Size: 135 32 686 307 213 19
Fragments arranged by size:
686 307 213 135 32 19
SmlI C'TyrA_G
Cuts at: 0 502 1050 1392
Size: 502 548 342
Fragments arranged by size:
548 502 342
SnaBI TAC'GTA
Cuts at: 0 1098 1392
Size: 1098 294
SphI G_CATG'C
Cuts at: 0 726 1392
Size: 726 666
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 1392
Size: 438 954
TatI w'GTAC_w
Cuts at: 0 272 1046 1328 1392
Size: 272 774 282 64
Fragments arranged by size:
774 282 272 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 1392
Size: 270 32 1090
Fragments arranged by size:
1090 270 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 1392
Size: 213 1179
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApoI AvaI BamHI
BanII BbsI BbvCI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI Eco57MI EcoO109I FalI FspI FspAI Hin4I
HincII MscI MslI MspA1I NspI PpuMI PstI PvuII
ScaI SfcI SmlI SnaBI SphI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BsaBI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI