Restriction map for pF1KB8603
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       AccI        AccII       AciI        AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BcgI        BciVI       
BclI        BglI        BglII       BlnI        BpmI        
BsaAI       BsaBI       BsaHI       BsaI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoT14I     FauI        FbaI        FseI        
FspI        Hin1I       HindIII     HpaI        HphI        
KasI        KspI        MamI        MluI        MluNI       
MscI        MspA1I      MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        110  GGTACC               G^GTACC
AccIII        671  TCCGGA               T^CCGGA
AflII         289  CTTAAG               C^TTAAG
AlwNI        1184  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1112  AGCGCT               AGC^GCT
Asp718I       110  GGTACC               G^GTACC
AspHI         939  GAGCAC               G[AT]GC[AT]^C
AvaI          932  CTCGAG               C^[CT]CG[GA]G
AvaII         354  GGACC                G^G[AT]CC
BamHI         550  GGATCC               G^GATCC
BanII         922  GGGCTC               G[GA]GC[CT]^C
BbsI          871  GAAGAC               GAAGAC(2/6)
BbvCI         713  CCTCAGC              CCTCAGC(-5/-2)
BcnI          427  CCCGG                CC^[GC]GG
BfaI          572  CTAG                 C^TAG
BfrI          289  CTTAAG               C^TTAAG
BlpI          309  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      309  GCTGAGC              GC^T[ACGT]AGC
BpuAI         871  GAAGAC               GAAGAC(2/6)
BsaJI         167  CCAGGG               C^C[ACGT][ACGT]GG
BsaWI         671  TCCGGA               [AT]^CCGG[AT]
BseAI         671  TCCGGA               T^CCGGA
BsgI          191  GTGCAG               GTGCAG(16/14)
BsiHKAI       939  GAGCAC               G[AT]GC[AT]^C
BsoBI         932  CTCGAG               C^[CT]CG[GA]G
BspEI         671  TCCGGA               T^CCGGA
BspMI         122  ACCTGC               ACCTGC(4/8)
BstAPI        376  GCATCACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         838  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         309  GCTGAGC              GC^T[ACGT]AGC
Eco47III     1112  AGCGCT               AGC^GCT
EcoRI         196  GAATTC               G^AATTC
EcoRV         784  GATATC               GAT^ATC
EcoT22I       374  ATGCAT               ATGCA^T
HaeII        1112  AGCGCT               [GA]GCGC^[CT]
HgaI          241  GACGC                GACGC(5/10)
HincII       1060  GTTGAC               GT[CT]^[GA]AC
HindII       1060  GTTGAC               GT[CT]^[GA]AC
KpnI          110  GGTACC               GGTAC^C
MaeI          572  CTAG                 C^TAG
MfeI         1164  CAATTG               C^AATTG
MroI          671  TCCGGA               T^CCGGA
MslI          471  CATTGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1164  CAATTG               C^AATTG
NciI          427  CCCGG                CC^[GC]GG
NsiI          374  ATGCAT               ATGCA^T
PaeR7I        932  CTCGAG               C^TCGAG
PleI          950  GACTC                GAGTC(4/5)
PstI          257  CTGCAG               CTGCA^G
SexAI        1049  ACCTGGT              A^CC[AT]GGT
XhoI          932  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI            4  GAATTT               [GA]^AATT[CT]
              196  GAATTC              
AfaI          111  GTAC                 GT^AC
             1217  GTAC                
ApoI            4  GAATTT               [GA]^AATT[CT]
              196  GAATTC              
Asp700I       418  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              679  GAACTGATTC          
BanI          110  GGTACC               G^G[CT][GA]CC
              975  GGTGCC              
BseRI         748  GAGGAG               GAGGAG(10/8)
              857  GAGGAG              
BsiYI         421  CCTGTTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1220  CCCACTTGAGG         
BslI          421  CCTGTTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1220  CCCACTTGAGG         
BsmFI         732  GGGAC                GGGAC(10/14)
              948  GGGAC               
BsmI          846  GAATGC               GAATGC(1/-1)
               65  GCATTC              
BspHI         911  TCATGA               T^CATGA
             1175  TCATGA              
Cfr13I        354  GGACC                G^G[ACGT]CC
             1075  GGGCC               
EarI          502  CTCTTC               CTCTTC(1/4)
              532  GAAGAG              
HaeIII        403  GGCC                 GG^CC
             1076  GGCC                
HapII         428  CCGG                 C^CGG
              672  CCGG                
HpaII         428  CCGG                 C^CGG
              672  CCGG                
Ksp632I       502  CTCTTC               CTCTTC(1/4)
              532  GAAGAG              
MaeII         332  ACGT                 A^CGT
              579  ACGT                
MspI          428  CCGG                 C^CGG
              672  CCGG                
RcaI          911  TCATGA               T^CATGA
             1175  TCATGA              
RsaI          111  GTAC                 GT^AC
             1217  GTAC                
SapI          532  GAAGAGC              GCTCTTC(1/4)
              501  GCTCTTC             
Sau96I        354  GGACC                G^G[ACGT]CC
             1075  GGGCC               
TaqI          855  TCGA                 T^CGA
              933  TCGA                
TfiI          262  GATTC                G^A[AT]TC
              684  GATTC               
TthHB8I       855  TCGA                 T^CGA
              933  TCGA                
XmnI          418  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              679  GAACTGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AluI        AlwI        BbvI        BmyI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       CfoI        DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HhaI        HinP1I      HinfI       
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      ScrFI       SfaNI       
SfcI        SmlI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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