Restriction map for
pF1KB8603
(Circular) (Six-base) MAPSORT of: pF1KB8603.seq Check: 5128 from: 1 to: 4349
pF1KB8603 4349 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 218 1365 218
Size: 1147 3202
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 2174 2606 2959 3621 901
Size: 1273 432 353 662 1629
Fragments arranged by size:
1629 1273 662 432 353
AfeI AGC'GCT
Cuts at: 1222 1222
Size: 4349
AflII C'TTAA_G
Cuts at: 397 397
Size: 4349
AflIII A'CryG_T
Cuts at: 1701 3501 3782 1701
Size: 1800 281 2268
Fragments arranged by size:
2268 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1109 1141 1109
Size: 32 4317
AlwNI CAG_nnn'CTG
Cuts at: 1297 1654 3092 1297
Size: 357 1438 2554
Fragments arranged by size:
2554 1438 357
ApaLI G'TGCA_C
Cuts at: 3187 3187
Size: 4349
ApoI r'AATT_y
Cuts at: 112 304 1353 3702 3958 112
Size: 192 1049 2349 256 503
Fragments arranged by size:
2349 1049 503 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 1040 1369 1040
Size: 329 4020
BamHI G'GATC_C
Cuts at: 658 1374 658
Size: 716 3633
BanI G'GyrC_C
Cuts at: 218 1083 1365 1779 2027 2062 218
Size: 865 282 414 248 35 2505
Fragments arranged by size:
2505 865 414 282 248 35
BanII G_rGCy'C
Cuts at: 1034 1363 2393 1034
Size: 329 1030 2990
Fragments arranged by size:
2990 1030 329
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 986 986
Size: 4349
BbvCI CC'TCA_GC
Cuts at: 822 822
Size: 4349
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3303 4349 2247
Size: 1056 1046 2247
Fragments arranged by size:
2247 1056 1046
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BfrBI ATG'CAT
Cuts at: 484 484
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 418 1461 418
Size: 1043 3306
Bme1580I G_kGCm'C
Cuts at: 1088 1974 2067 3191 1088
Size: 886 93 1124 2246
Fragments arranged by size:
2246 1124 886 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1561 1642 1977 4121 1063
Size: 498 81 335 2144 1291
Fragments arranged by size:
2144 1291 498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
Bpu10I CC'TnA_GC
Cuts at: 822 822
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1353 1525 2404 3118 3416 256
Size: 1097 172 879 714 298 1189
Fragments arranged by size:
1189 1097 879 714 298 172
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 779 1538 2059 2737 3148 3295 3569 779
Size: 759 521 678 411 147 274 1559
Fragments arranged by size:
1559 759 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 1294 1324 868
Size: 30 396 30 3893
Fragments arranged by size:
3893 396 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 980 871
Size: 109 4240
BseYI C'CCAG_C
Cuts at: 1774 2532 3197 3605 1774
Size: 758 665 408 2518
Fragments arranged by size:
2518 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 320
Size: 4349
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3167 104
Size: 1787 46 1230 1286
Fragments arranged by size:
1787 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1363 2141 2331 3191 1051
Size: 312 778 190 860 2209
Fragments arranged by size:
2209 860 778 312 190
BsmI GAATG_Cn'
Cuts at: 173 960 173
Size: 787 3562
Bsp1286I G_dGCh'C
Cuts at: 1034 1051 1088 1363 1974 2067 2141 2331 2393
Size: 17 37 275 611 93 74 190 62
Cuts at: 2393 3191 1034
Size: 798 2192
Fragments arranged by size:
2192 798 611 275 190 93 74 62
37 17
BspEI T'CCGG_A
Cuts at: 779 779
Size: 4349
BspHI T'CATG_A
Cuts at: 1019 1283 2781 1019
Size: 264 1498 2587
Fragments arranged by size:
2587 1498 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 1399 1613 1915 2296 239
Size: 1160 214 302 381 2292
Fragments arranged by size:
2292 1160 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 2347 2528 2347
Size: 181 4168
BssSI C'ACGA_G
Cuts at: 2620 3328 2620
Size: 708 3641
BstAPI GCAn_nnn'nTGC
Cuts at: 490 1726 490
Size: 1236 3113
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstYI r'GATC_y
Cuts at: 1 145 658 861 1374 1841 1864 2199 2445
Size: 144 513 203 513 467 23 335 246
Cuts at: 2445 2751 2763 2849 3901 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 513 513 467 449 335 306 246
203 144 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1592 566
Size: 1026 3323
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 3399 2055
Size: 1344 3005
EaeI y'GGCC_r
Cuts at: 1934 2108 2499 2526 3871 1934
Size: 174 391 27 1345 2412
Fragments arranged by size:
2412 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 616 635 2372 2582 616
Size: 19 1737 210 2383
Fragments arranged by size:
2383 1737 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 3299 3445 4159 2557
Size: 742 146 714 2747
Fragments arranged by size:
2747 742 714 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 2174 2606 2959 3621 901
Size: 1273 432 353 662 1629
Fragments arranged by size:
1629 1273 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1488 1488
Size: 4349
EcoRI G'AATT_C
Cuts at: 304 1353 304
Size: 1049 3300
EcoRV GAT'ATC
Cuts at: 894 894
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1224 1783 2031 3261 4032 1224
Size: 559 248 1230 771 1541
Fragments arranged by size:
1541 1230 771 559 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 411 443 868 900 1195 1227 2191 2221 2223
Size: 32 425 32 295 32 964 30 2
Cuts at: 2223 2253 411
Size: 30 2507
Fragments arranged by size:
2507 964 425 295 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1170 1388 1170
Size: 218 4131
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 222 1369 222
Size: 1147 3202
MfeI C'AATT_G
Cuts at: 1272 1272
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 1143 3108 3292 225
Size: 918 1965 184 1282
Fragments arranged by size:
1965 1282 918 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 583 2338 2465 583
Size: 1755 127 2467
Fragments arranged by size:
2467 1755 127
MspA1I CmG'CkG
Cuts at: 1460 1774 2134 2918 3163 1460
Size: 314 360 784 245 2646
Fragments arranged by size:
2646 784 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NsiI A_TGCA'T
Cuts at: 486 486
Size: 4349
NspI r_CATG'y
Cuts at: 1402 1902 3505 1402
Size: 500 1603 2246
Fragments arranged by size:
2246 1603 500
PciI A'CATG_T
Cuts at: 3501 3501
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 4317
PsiI TTA'TAA
Cuts at: 907 907
Size: 4349
PstI C_TGCA'G
Cuts at: 369 1396 2081 369
Size: 1027 685 2637
Fragments arranged by size:
2637 1027 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 1774 2134 1774
Size: 360 3989
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 616 635 2372 2582 616
Size: 19 1737 210 2383
Fragments arranged by size:
2383 1737 210 19
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SexAI A'CCwGG_T
Cuts at: 1157 1157
Size: 4349
SfcI C'TryA_G
Cuts at: 33 365 667 897 1392 2077 3045 3236 33
Size: 332 302 230 495 685 968 191 1146
Fragments arranged by size:
1146 968 685 495 332 302 230 191
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 235 397 1040 1332 1504 2419 3133 3395 235
Size: 162 643 292 172 915 714 262 1189
Fragments arranged by size:
1189 915 714 643 292 262 172 162
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 1483 1837 107
Size: 1376 354 2619
Fragments arranged by size:
2619 1376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1932 2065 2524 2733 1932
Size: 133 459 209 3548
Fragments arranged by size:
3548 459 209 133
TatI w'GTAC_w
Cuts at: 3864 3864
Size: 4349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XhoI C'TCGA_G
Cuts at: 1040 1040
Size: 4349
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
XmnI GAAnn'nnTTC
Cuts at: 530 791 530
Size: 261 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BpmI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI