Restriction map for pF1KB8589
 (Linear) (Six-base) MAPSORT of: KIBB8589.seq  Check: 5887  from: 1  to: 1074



KIBB8589.orf

 With 182 enzymes: SgfI * 

                        July 2, 2008 13:23  ..

AatII G_ACGT'C

Cuts at:      0     483    1074 
   Size:        483     591
 
AccI GT'mk_AC

Cuts at:      0     219    1074 
   Size:        219     855
 
Acc65I G'GTAC_C

Cuts at:      0     828    1074 
   Size:        828     246
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1074 
   Size:        803     271
 
AflIII A'CryG_T

Cuts at:      0     124    1074 
   Size:        124     950
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     614     646    1074 
   Size:        614      32     428

  Fragments arranged by size: 

                 614     428      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1066    1074 
   Size:       1066       8
 
ApaLI G'TGCA_C

Cuts at:      0    1056    1074 
   Size:       1056      18
 
ApoI r'AATT_y

Cuts at:      0     227    1074 
   Size:        227     847
 
AvaI C'yCGr_G

Cuts at:      0     232    1074 
   Size:        232     842
 
BanI G'GyrC_C

Cuts at:      0     828    1074 
   Size:        828     246
 
BanII G_rGCy'C

Cuts at:      0     586    1074 
   Size:        586     488
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     638     734    1074 
   Size:        638      96     340

  Fragments arranged by size: 

                 638     340      96
 
BclI T'GATC_A

Cuts at:      0      21    1074 
   Size:         21    1053
 
BfrBI ATG'CAT

Cuts at:      0     310    1074 
   Size:        310     764
 
BglII A'GATC_T

Cuts at:      0    1033    1074 
   Size:       1033      41
 
Bme1580I G_kGCm'C

Cuts at:      0    1060    1074 
   Size:       1060      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1074 
   Size:        262     812
 
BsaHI Gr'CG_yC

Cuts at:      0     480    1074 
   Size:        480     594
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     614     644    1074 
   Size:        614      30     430

  Fragments arranged by size: 

                 614     430      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65    1074 
   Size:         65    1009
 
BsiEI CG_ry'CG

Cuts at:      0     969     982    1074 
   Size:        969      13      92

  Fragments arranged by size: 

                 969      92      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     213     586    1060    1074 
   Size:        213     373     474      14

  Fragments arranged by size: 

                 474     373     213      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     213     586    1060    1074 
   Size:        213     373     474      14

  Fragments arranged by size: 

                 474     373     213      14
 
BspHI T'CATG_A

Cuts at:      0     804    1074 
   Size:        804     270
 
BsrBI CCG'CTC

Cuts at:      0     598    1074 
   Size:        598     476
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     904    1074 
   Size:        904     170
 
BstYI r'GATC_y

Cuts at:      0    1033    1074 
   Size:       1033      41
 
ClaI AT'CG_AT

Cuts at:      0     969     978     993    1074 
   Size:        969       9      15      81

  Fragments arranged by size: 

                 969      81      15       9
 
DraIII CAC_nnn'GTG

Cuts at:      0     619    1074 
   Size:        619     455
 
EcoICRI GAG'CTC

Cuts at:      0     584    1074 
   Size:        584     490
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1074 
   Size:        803     271
 
EcoO109I rG'GnC_Cy

Cuts at:      0     656    1074 
   Size:        656     418
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     273    1074 
   Size:        273     801
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624     793     825     877     909    1074 
   Size:        592      32     169      32      52      32     165

  Fragments arranged by size: 

                 592     169     165      52      32      32      32
 
HincII GTy'rAC

Cuts at:      0     220     683    1074 
   Size:        220     463     391

  Fragments arranged by size: 

                 463     391     220
 
HpaI GTT'AAC

Cuts at:      0     683    1074 
   Size:        683     391
 
KpnI G_GTAC'C

Cuts at:      0     832    1074 
   Size:        832     242
 
MslI CAynn'nnrTG

Cuts at:      0     406    1074 
   Size:        406     668
 
MspA1I CmG'CkG

Cuts at:      0     769    1074 
   Size:        769     305
 
NsiI A_TGCA'T

Cuts at:      0     312    1074 
   Size:        312     762
 
NspI r_CATG'y

Cuts at:      0      39     128    1074 
   Size:         39      89     946

  Fragments arranged by size: 

                 946      89      39
 
PciI A'CATG_T

Cuts at:      0     124    1074 
   Size:        124     950
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     614     646    1074 
   Size:        614      32     428

  Fragments arranged by size: 

                 614     428      32
 
PstI C_TGCA'G

Cuts at:      0     900    1074 
   Size:        900     174
 
PvuI CG_AT'CG

Cuts at:      0     969     982    1074 
   Size:        969      13      92

  Fragments arranged by size: 

                 969      92      13
 
PvuII CAG'CTG

Cuts at:      0     769    1074 
   Size:        769     305
 
SacI G_AGCT'C

Cuts at:      0     586    1074 
   Size:        586     488
 
SalI G'TCGA_C

Cuts at:      0     218    1074 
   Size:        218     856
 
SfcI C'TryA_G

Cuts at:      0     896    1074 
   Size:        896     178
 
SmlI C'TyrA_G

Cuts at:      0     277    1074 
   Size:        277     797
 
SpeI A'CTAG_T

Cuts at:      0     358    1074 
   Size:        358     716
 
StuI AGG'CCT

Cuts at:      0    1044    1074 
   Size:       1044      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     323    1074 
   Size:        323     751
 
XbaI T'CTAG_A

Cuts at:      0    1036    1074 
   Size:       1036      38
 
ZraI GAC'GTC

Cuts at:      0     481    1074 
   Size:        481     593
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AloI    AlwNI    ApaLI
     ApoI     AvaI     BanI    BanII     BbsI     BclI    BfrBI    BglII
 Bme1580I    BpuEI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BsrBI   BstAPI    BstYI     ClaI   DraIII  EcoICRI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII     HpaI     KpnI     MslI   MspA1I
     NsiI     NspI     PciI     PpiI     PstI     PvuI    PvuII     SacI
     SalI     SfcI     SmlI     SpeI     StuI    TaqII     XbaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BseRI
    BseYI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI
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