Restriction map for pF1KB8587
 (Linear) (Six-base) MAPSORT of: KIBB8587.seq  Check: 4762  from: 1  to: 711



KIBB8587.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     604     711 
   Size:        248     356     107

  Fragments arranged by size: 

                 356     248     107
 
AhdI GACnn_n'nnGTC

Cuts at:      0     276     711 
   Size:        276     435
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     413     445     711 
   Size:        413      32     266

  Fragments arranged by size: 

                 413     266      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     533     711 
   Size:        533     178
 
ApaI G_GGCC'C

Cuts at:      0      69     711 
   Size:         69     642
 
ApoI r'AATT_y

Cuts at:      0     367     423     553     711 
   Size:        367      56     130     158

  Fragments arranged by size: 

                 367     158     130      56
 
AvaI C'yCGr_G

Cuts at:      0      68     711 
   Size:         68     643
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     520     553     711 
   Size:        520      33     158

  Fragments arranged by size: 

                 520     158      33
 
BanII G_rGCy'C

Cuts at:      0      22      69     407     711 
   Size:         22      47     338     304

  Fragments arranged by size: 

                 338     304      47      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     706     711 
   Size:        706       5
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     548     711 
   Size:        548     163
 
BglI GCCn_nnn'nGGC

Cuts at:      0     100     711 
   Size:        100     611
 
BlpI GC'TnA_GC

Cuts at:      0     119     711 
   Size:        119     592
 
Bme1580I G_kGCm'C

Cuts at:      0      69     588     711 
   Size:         69     519     123

  Fragments arranged by size: 

                 519     123      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     462     711 
   Size:        462     249
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     134     711 
   Size:        134     577
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     163     383     711 
   Size:          6     157     220     328

  Fragments arranged by size: 

                 328     220     157       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     386     711 
   Size:        386     325
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546     711 
   Size:        546     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     511     711 
   Size:        511     200
 
Bsp1286I G_dGCh'C

Cuts at:      0      22      69     407     511     588     711 
   Size:         22      47     338     104      77     123

  Fragments arranged by size: 

                 338     123     104      77      47      22
 
BspHI T'CATG_A

Cuts at:      0     432     473     711 
   Size:        432      41     238

  Fragments arranged by size: 

                 432     238      41
 
BsrDI GCAATG_nn'

Cuts at:      0     346     711 
   Size:        346     365
 
BtgI C'CryG_G

Cuts at:      0      95     711 
   Size:         95     616
 
BtsI GCAGTG_nn'

Cuts at:      0     332     711 
   Size:        332     379
 
EarI CTCTTCn'nnn_

Cuts at:      0     661     711 
   Size:        661      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     163     248     383     604     711 
   Size:          6     157      85     135     221     107

  Fragments arranged by size: 

                 221     157     135     107      85       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     319     711 
   Size:        319     392
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     232     711 
   Size:         65     167     479

  Fragments arranged by size: 

                 479     167      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     262     294     711 
   Size:        177      32      53      32     417

  Fragments arranged by size: 

                 417     177      53      32      32
 
MspA1I CmG'CkG

Cuts at:      0     123     711 
   Size:        123     588
 
NcoI C'CATG_G

Cuts at:      0      95     711 
   Size:         95     616
 
NspI r_CATG'y

Cuts at:      0     279     465     711 
   Size:        279     186     246

  Fragments arranged by size: 

                 279     246     186
 
PasI CC'CwG_GG

Cuts at:      0      15     711 
   Size:         15     696
 
PpuMI rG'GwC_Cy

Cuts at:      0     232     711 
   Size:        232     479
 
PsiI TTA'TAA

Cuts at:      0     247     711 
   Size:        247     464
 
PspOMI G'GGCC_C

Cuts at:      0      65     711 
   Size:         65     646
 
StyI C'CwwG_G

Cuts at:      0      95     320     711 
   Size:         95     225     391

  Fragments arranged by size: 

                 391     225      95
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     123     711 
   Size:        123     588
 
TatI w'GTAC_w

Cuts at:      0     337     711 
   Size:        337     374
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApaI     ApoI     AvaI     BaeI
    BanII     BbsI    BciVI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI     BtgI
     BtsI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I   MspA1I     NcoI
     NspI     PasI    PpuMI     PsiI   PspOMI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII
    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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