Restriction map for pF1KB8585
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccII       AcyI        AflIII      
AgeI        AlwNI       Aor51HI     ApaI        AscI        
Asp718I     AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AccI          235  GTCTAC               GT^[AC][GT]AC
AccIII        158  TCCGGA               T^CCGGA
AclI          707  AACGTT               AA^CGTT
AflII        1740  CTTAAG               C^TTAAG
AhdI         1285  GACTACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2018  GTGCAC               G^TGCAC
AlwI         1438  GATCC                GGATC(4/5)
AocI           22  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2018  GTGCAC               G^TGCAC
AseI         1063  ATTAAT               AT^TAAT
AsnI         1063  ATTAAT               AT^TAAT
Asp700I      1914  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1285  GACTACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          203  TGGCCA               TGG^CCA
BciVI        1390  GTATCC               GTATCC(6/5)
BfrI         1740  CTTAAG               C^TTAAG
BglII         276  AGATCT               A^GATCT
BsaI         2377  GGTCTC               GGTCTC(1/5)
BsaWI         158  TCCGGA               [AT]^CCGG[AT]
BseAI         158  TCCGGA               T^CCGGA
BseRI        2062  GAGGAG               GAGGAG(10/8)
BsmI          587  GAATGC               GAATGC(1/-1)
Bsp1407I      291  TGTACA               T^GTACA
BspEI         158  TCCGGA               T^CCGGA
BspMI         990  GCAGGT               ACCTGC(4/8)
BsrDI         897  CATTGC               GCAATG(2/0)
BsrGI         291  TGTACA               T^GTACA
BstAPI       1762  GCACTAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         446  TTCGAA               TT^CGAA
BstYI         276  AGATCT               [GA]^GATC[CT]
Bsu36I         22  CCTCAGG              CC^T[ACGT]AGG
DraI         2226  TTTAAA               TTT^AAA
DraII        1502  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          203  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1285  GACTACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         22  CCTCAGG              CC^T[ACGT]AGG
EcoNI         484  CCTGATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1502  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI         2340  GCGGG                CCCGC(4/6)
HapII         159  CCGG                 C^CGG
HincII       1216  GTTGAC               GT[CT]^[GA]AC
HindII       1216  GTTGAC               GT[CT]^[GA]AC
HindIII       112  AAGCTT               A^AGCTT
HpaII         159  CCGG                 C^CGG
MflI          276  AGATCT               [GA]^GATC[CT]
MluNI         203  TGGCCA               TGG^CCA
MroI          158  TCCGGA               T^CCGGA
MscI          203  TGGCCA               TGG^CCA
MslI          868  CACCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          159  CCGG                 C^CGG
NcoI          536  CCATGG               C^CATGG
NdeI         1808  CATATG               CA^TATG
NheI         1872  GCTAGC               G^CTAGC
NspV          446  TTCGAA               TT^CGAA
PpuMI        1502  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        1063  ATTAAT               AT^TAAT
Psp1406I      707  AACGTT               AA^CGTT
PvuII        1612  CAGCTG               CAG^CTG
SexAI        1364  ACCAGGT              A^CC[AT]GGT
SfuI          446  TTCGAA               TT^CGAA
SspBI         291  TGTACA               T^GTACA
SspI         1231  AATATT               AAT^ATT
Tsp45I        521  GTGAC                ^GT[GC]AC
XcmI         1376  CCATAATGAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         276  AGATCT               [GA]^GATC[CT]
XmnI         1914  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BbsI         1429  GAAGAC               GAAGAC(2/6)
             2254  GAAGAC              
BglI           55  GCCATACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1466  GCCTTGTGGGC         
BpmI          826  CTCCAG               CTGGAG(16/14)
             2320  CTGGAG              
BpuAI        1429  GAAGAC               GAAGAC(2/6)
             2254  GAAGAC              
BspHI        1794  TCATGA               T^CATGA
             1935  TCATGA              
DsaI          411  CCGTGG               C^C[GA][CT]GG
              536  CCATGG              
EcoRI        1635  GAATTC               G^AATTC
             1813  GAATTC              
EcoT14I       316  CCAAGG               C^C[AT][AT]GG
              536  CCATGG              
MaeII         708  ACGT                 A^CGT
             1396  ACGT                
NspI          132  ACATGC               [GA]CATG^[CT]
             1022  ACATGC              
PleI         1950  GACTC                GAGTC(4/5)
             1291  GAGTC               
RcaI         1794  TCATGA               T^CATGA
             1935  TCATGA              
ScaI         1277  AGTACT               AGT^ACT
             1517  AGTACT              
StyI          316  CCAAGG               C^C[AT][AT]GG
              536  CCATGG              
XbaI         1012  TCTAGA               T^CTAGA
             1116  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaII       BbvI        BclI        BfaI        
BmyI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FbaI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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