Restriction map for pF1KB8585
 (Linear) (Six-base) MAPSORT of: KIBB8585.seq  Check: 8982  from: 1  to: 2388



KIBB8585.orf

 With 182 enzymes: SgfI * 

                        November 4, 2009 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     981    2388 
   Size:        981    1407
 
AccI GT'mk_AC

Cuts at:      0     236    2388 
   Size:        236    2152
 
AclI AA'CG_TT

Cuts at:      0     708    2388 
   Size:        708    1680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1013    1905    2388 
   Size:        445     568     892     483

  Fragments arranged by size: 

                 892     568     483     445
 
AflII C'TTAA_G

Cuts at:      0    1740    2388 
   Size:       1740     648
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1290    2388 
   Size:       1290    1098
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2142    2388 
   Size:       2142     246
 
ApaLI G'TGCA_C

Cuts at:      0    2018    2388 
   Size:       2018     370
 
ApoI r'AATT_y

Cuts at:      0     650     666     817    1383    1635    1813    2165    2388 
   Size:        650      16     151     566     252     178     352     223

  Fragments arranged by size: 

                 650     566     352     252     223     178     151      16
            
 
AseI AT'TA_AT

Cuts at:      0    1064    2388 
   Size:       1064    1324
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1436    2261    2388 
   Size:       1436     825     127

  Fragments arranged by size: 

                1436     825     127
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1401    2388 
   Size:       1401     987
 
BclI T'GATC_A

Cuts at:      0     332    1712    1889    1992    2388 
   Size:        332    1380     177     103     396

  Fragments arranged by size: 

                1380     396     332     177     103
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61    1472    2388 
   Size:         61    1411     916

  Fragments arranged by size: 

                1411     916      61
 
BglII A'GATC_T

Cuts at:      0     276    2388 
   Size:        276    2112
 
Bme1580I G_kGCm'C

Cuts at:      0    1962    2022    2388 
   Size:       1962      60     366

  Fragments arranged by size: 

                1962     366      60
 
BmtI G_CTAG'C

Cuts at:      0    1876    2388 
   Size:       1876     512
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2275    2388 
   Size:       2275     113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    2341    2388 
   Size:        811    1530      47

  Fragments arranged by size: 

                1530     811      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     450     758    1343    2388 
   Size:        450     308     585    1045

  Fragments arranged by size: 

                1045     585     450     308
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2383    2388 
   Size:       2383       5
 
BsaWI w'CCGG_w

Cuts at:      0     158    2388 
   Size:        158    2230
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2077    2388 
   Size:       2077     311
 
BseYI C'CCAG_C

Cuts at:      0     954    1771    2388 
   Size:        954     817     617

  Fragments arranged by size: 

                 954     817     617
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1196    2170    2388 
   Size:        205     991     974     218

  Fragments arranged by size: 

                 991     974     218     205
 
BsiHKAI G_wGCw'C

Cuts at:      0    1764    2014    2022    2388 
   Size:       1764     250       8     366

  Fragments arranged by size: 

                1764     366     250       8
 
BsmI GAATG_Cn'

Cuts at:      0     593    2388 
   Size:        593    1795
 
Bsp1286I G_dGCh'C

Cuts at:      0    1764    1962    2014    2022    2388 
   Size:       1764     198      52       8     366

  Fragments arranged by size: 

                1764     366     198      52       8
 
BspEI T'CCGG_A

Cuts at:      0     158    2388 
   Size:        158    2230
 
BspHI T'CATG_A

Cuts at:      0    1794    1935    2388 
   Size:       1794     141     453

  Fragments arranged by size: 

                1794     453     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     981    2388 
   Size:        981    1407
 
BsrDI GCAATG_nn'

Cuts at:      0     896    2388 
   Size:        896    1492
 
BsrGI T'GTAC_A

Cuts at:      0     291    2388 
   Size:        291    2097
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1768    2388 
   Size:       1768     620
 
BstBI TT'CG_AA

Cuts at:      0     447    2388 
   Size:        447    1941
 
BstYI r'GATC_y

Cuts at:      0     276    2388 
   Size:        276    2112
 
Bsu36I CC'TnA_GG

Cuts at:      0      23    2388 
   Size:         23    2365
 
BtgI C'CryG_G

Cuts at:      0     411     536    2388 
   Size:        411     125    1852

  Fragments arranged by size: 

                1852     411     125
 
BtsI GCAGTG_nn'

Cuts at:      0    1582    2388 
   Size:       1582     806
 
DraI TTT'AAA

Cuts at:      0    2228    2388 
   Size:       2228     160
 
EaeI y'GGCC_r

Cuts at:      0     203    2388 
   Size:        203    2185
 
EarI CTCTTCn'nnn_

Cuts at:      0      85    1922    2032    2388 
   Size:         85    1837     110     356

  Fragments arranged by size: 

                1837     356     110      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     811    1013    1905    2341    2388 
   Size:        445     366     202     892     436      47

  Fragments arranged by size: 

                 892     445     436     366     202      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     488    2388 
   Size:        488    1900
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1503    2388 
   Size:       1503     885
 
EcoRI G'AATT_C

Cuts at:      0    1635    1813    2388 
   Size:       1635     178     575

  Fragments arranged by size: 

                1635     575     178
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2170    2388 
   Size:       2170     218
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     822     854     935     967    1027    1059    1276    1308 
   Size:        822      32      81      32      60      32     217      32

Cuts at:   1308    1392    1424    2388 
   Size:         84      32     964

  Fragments arranged by size: 

                 964     822     217      84      81      60      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1218    2388 
   Size:       1218    1170
 
HindIII A'AGCT_T

Cuts at:      0     112    2388 
   Size:        112    2276
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     668    2388 
   Size:        321     347    1720

  Fragments arranged by size: 

                1720     347     321
 
MscI TGG'CCA

Cuts at:      0     205    2388 
   Size:        205    2183
 
MslI CAynn'nnrTG

Cuts at:      0     872    2388 
   Size:        872    1516
 
MspA1I CmG'CkG

Cuts at:      0     958    1569    1614    2388 
   Size:        958     611      45     774

  Fragments arranged by size: 

                 958     774     611      45
 
NcoI C'CATG_G

Cuts at:      0     536    2388 
   Size:        536    1852
 
NdeI CA'TA_TG

Cuts at:      0    1809    2388 
   Size:       1809     579
 
NheI G'CTAG_C

Cuts at:      0    1872    2388 
   Size:       1872     516
 
NspI r_CATG'y

Cuts at:      0     136    1026    2388 
   Size:        136     890    1362

  Fragments arranged by size: 

                1362     890     136
 
PfoI T'CCnGG_A

Cuts at:      0     373    2388 
   Size:        373    2015
 
PpuMI rG'GwC_Cy

Cuts at:      0    1503    2388 
   Size:       1503     885
 
PsiI TTA'TAA

Cuts at:      0    1340    2388 
   Size:       1340    1048
 
PvuII CAG'CTG

Cuts at:      0    1614    2388 
   Size:       1614     774
 
ScaI AGT'ACT

Cuts at:      0    1279    1519    2388 
   Size:       1279     240     869

  Fragments arranged by size: 

                1279     869     240
 
SexAI A'CCwGG_T

Cuts at:      0    1364    2388 
   Size:       1364    1024
 
SfcI C'TryA_G

Cuts at:      0     756     998    1424    2388 
   Size:        756     242     426     964

  Fragments arranged by size: 

                 964     756     426     242
 
SmlI C'TyrA_G

Cuts at:      0     429     773    1358    1740    2388 
   Size:        429     344     585     382     648

  Fragments arranged by size: 

                 648     585     429     382     344
 
SspI AAT'ATT

Cuts at:      0    1233    2388 
   Size:       1233    1155
 
StyI C'CwwG_G

Cuts at:      0     316     536    2388 
   Size:        316     220    1852

  Fragments arranged by size: 

                1852     316     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     570    1280    2388 
   Size:        570     710    1108

  Fragments arranged by size: 

                1108     710     570
 
TatI w'GTAC_w

Cuts at:      0     291     546    1277    1517    2388 
   Size:        291     255     731     240     871

  Fragments arranged by size: 

                 871     731     291     255     240
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     254     286    1941    1973    2388 
   Size:        254      32    1655      32     415

  Fragments arranged by size: 

                1655     415     254      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1012    1116    2388 
   Size:       1012     104    1272

  Fragments arranged by size: 

                1272    1012     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1383    2388 
   Size:       1383    1005
 
XmnI GAAnn'nnTTC

Cuts at:      0    1918    2388 
   Size:       1918     470
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI    AflII     AhdI     AlfI    ApaLI
     ApoI     AseI     BbsI    BciVI     BclI     BglI    BglII Bme1580I
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BstAPI    BstBI    BstYI   Bsu36I     BtgI     BtsI     DraI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NspI     PfoI    PpuMI     PsiI    PvuII     ScaI    SexAI
     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI   AflIII     AgeI     AleI     AloI    AlwNI
     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BfrBI     BlpI    BmgBI     BmrI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI    BsmBI
    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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